TY - JOUR
T1 - Loss of tumoral expression of soluble IL-6 receptor is associated with disease progression in colorectal cancer
AU - Okugawa, Y.
AU - Miki, C.
AU - Toiyama, Y.
AU - Yasuda, H.
AU - Yokoe, T.
AU - Saigusa, S.
AU - Hiro, J.
AU - Tanaka, K.
AU - Inoue, Y.
AU - Kusunoki, M.
PY - 2010/9/7
Y1 - 2010/9/7
N2 - Background:Interleukin-6 (IL-6) binds both the membrane and soluble forms of the IL-6 receptor (sIL-6R), which induces a complex with gp130, and proliferation of tumour cells. The aim of this study is to clarify the relationship between tumoral sIL-6R expression and disease progression in colorectal cancer patients.Methods:We measured tissue concentrations of sIL-6R in tumour and normal mucosa from 161 colorectal cancer patients undergoing surgery, and in supernatants from colon cancer cell lines. The expression of IL-6, IL-6R and gp130 was evaluated by immunohistochemical analysis.Results:Loss of tumour expression of sIL-6R as defined by sIL-6R Ca/N ratio 1.0 was significantly associated with factors reflecting disease progression, and was an independent prognostic factor not only in all the patients in this study, but also in the patients with curative intent. Colon cancer cell lines produced sIL-6R in vitro, and the production of sIL-6R in cancer cell lines was stimulated by cytokine stimulation. Immunohistochemistry revealed that loss of tumour expression of sIL-6R was significantly inversely correlated with intense IL-6 expression in the cytoplasm of cancer cells. In addition, tumoral IL-1Β expression was significantly correlated with sIL-6R expression.Conclusion:Loss of tumour expression of sIL-6R is associated with colorectal cancer disease progression.
AB - Background:Interleukin-6 (IL-6) binds both the membrane and soluble forms of the IL-6 receptor (sIL-6R), which induces a complex with gp130, and proliferation of tumour cells. The aim of this study is to clarify the relationship between tumoral sIL-6R expression and disease progression in colorectal cancer patients.Methods:We measured tissue concentrations of sIL-6R in tumour and normal mucosa from 161 colorectal cancer patients undergoing surgery, and in supernatants from colon cancer cell lines. The expression of IL-6, IL-6R and gp130 was evaluated by immunohistochemical analysis.Results:Loss of tumour expression of sIL-6R as defined by sIL-6R Ca/N ratio 1.0 was significantly associated with factors reflecting disease progression, and was an independent prognostic factor not only in all the patients in this study, but also in the patients with curative intent. Colon cancer cell lines produced sIL-6R in vitro, and the production of sIL-6R in cancer cell lines was stimulated by cytokine stimulation. Immunohistochemistry revealed that loss of tumour expression of sIL-6R was significantly inversely correlated with intense IL-6 expression in the cytoplasm of cancer cells. In addition, tumoral IL-1Β expression was significantly correlated with sIL-6R expression.Conclusion:Loss of tumour expression of sIL-6R is associated with colorectal cancer disease progression.
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U2 - 10.1038/sj.bjc.6605827
DO - 10.1038/sj.bjc.6605827
M3 - Article
C2 - 20823887
AN - SCOPUS:77956448658
SN - 0007-0920
VL - 103
SP - 787
EP - 795
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 6
ER -
