Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice

Shuichi Hayashi, Yoko Inoue, Satoko Takai, Mari Kaneko, Go Shioi, Tsuyoshi Miyakawa, Masatoshi Takeichi

Research output: Contribution to journalArticle

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Abstract

Mutations in the X-linked gene Protocadherin-19 (Pcdh19) cause female-limited epilepsy and mental retardation in humans. Although Pcdh19 is known to be a homophilic cell-cell adhesion molecule, how its mutations bring about female-specific disorders remains elusive. Here, we report the effects of Pcdh19 knockout in mice on their development and behavior. Pcdh19 was expressed in various brain regions including the cerebral cortex and hippocampus. Although Pcdh19-positive cells were evenly distributed in layer V of wild-type cortices, their distribution became a mosaic in Pcdh19 heterozygous female cortices. In cortical and hippocampal neurons, Pcdh19 was localized along their dendrites, showing occasional accumulation on synapses. Pcdh19 mutants, however, displayed no detectable abnormalities in dendrite and spine morphology of layer V neurons. Nevertheless, Pcdh19 hemizygous males and heterozygous females showed impaired behaviors including activity defects under stress conditions. Notably, only heterozygous females exhibited decreased fear responses. In addition, Pcdh19 overexpression in wild-type cortices led to ectopic clustering of Pcdh19-positive neurons. These results suggest that Pcdh19 is required for behavioral control in mice, but its genetic loss differentially affects the male and female behavior, as seen in human, and they also support the hypothesis that the mosaic expression of Pcdh19 in brains perturbs neuronal interactions.

Original languageEnglish
Article number5801
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 01-12-2017

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Dendrites
Neurons
X-Linked Genes
Mutation
Brain
Cell Adhesion Molecules
Knockout Mice
Intellectual Disability
Cerebral Cortex
Synapses
Fear
Cluster Analysis
Epilepsy
Hippocampus
Spine

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice",
abstract = "Mutations in the X-linked gene Protocadherin-19 (Pcdh19) cause female-limited epilepsy and mental retardation in humans. Although Pcdh19 is known to be a homophilic cell-cell adhesion molecule, how its mutations bring about female-specific disorders remains elusive. Here, we report the effects of Pcdh19 knockout in mice on their development and behavior. Pcdh19 was expressed in various brain regions including the cerebral cortex and hippocampus. Although Pcdh19-positive cells were evenly distributed in layer V of wild-type cortices, their distribution became a mosaic in Pcdh19 heterozygous female cortices. In cortical and hippocampal neurons, Pcdh19 was localized along their dendrites, showing occasional accumulation on synapses. Pcdh19 mutants, however, displayed no detectable abnormalities in dendrite and spine morphology of layer V neurons. Nevertheless, Pcdh19 hemizygous males and heterozygous females showed impaired behaviors including activity defects under stress conditions. Notably, only heterozygous females exhibited decreased fear responses. In addition, Pcdh19 overexpression in wild-type cortices led to ectopic clustering of Pcdh19-positive neurons. These results suggest that Pcdh19 is required for behavioral control in mice, but its genetic loss differentially affects the male and female behavior, as seen in human, and they also support the hypothesis that the mosaic expression of Pcdh19 in brains perturbs neuronal interactions.",
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Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice. / Hayashi, Shuichi; Inoue, Yoko; Takai, Satoko; Kaneko, Mari; Shioi, Go; Miyakawa, Tsuyoshi; Takeichi, Masatoshi.

In: Scientific reports, Vol. 7, No. 1, 5801, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Shioi, Go

AU - Miyakawa, Tsuyoshi

AU - Takeichi, Masatoshi

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