Low-autofluorescence fluoropolymer membrane filters for cell filtration

Naoto Kihara; Daiki Kuboyama; Daisuke Onoshima; Kenji Ishikawa; Hiromasa Tanaka; Naoya Ozawa; Tetsunari Hase; Ryohei Koguchi; Hiroshi Yukawa; Hidefumi Odaka; Yoshinori Hasegawa; Yoshinobu Baba; Masaru Hori

doi:10.7567/JJAP.57.06JF03

Low-autofluorescence fluoropolymer membrane filters for cell filtration

Naoto Kihara
, Daiki Kuboyama
, Daisuke Onoshima
, Kenji Ishikawa
, Hiromasa Tanaka
, Naoya Ozawa
, Tetsunari Hase
, Ryohei Koguchi
, Hiroshi Yukawa
, Hidefumi Odaka
, Yoshinori Hasegawa
, Yoshinobu Baba
, Masaru Hori

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A fluoropolymer membrane filter with through-holes was fabricated by photolithographic patterning and the dry etching method. 380,000 highly packed through-holes, each with a diameter of 7 µm were able to cover a whole area with a diameter of 13 mm. Ethylene tetrafluoroethylene (ETFE) was used as the membrane, which was suitable for the fluorescence detection of rare cells such as circulating tumor cells (CTCs) in human blood. The device fabrication for the size based capture of rare cells in blood such as CTCs is realized in this study.

Original language	English
Article number	06JF03
Journal	Japanese Journal of Applied Physics
Volume	57
Issue number	6
DOIs	https://doi.org/10.7567/JJAP.57.06JF03
Publication status	Published - 06-2018
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Engineering
General Physics and Astronomy

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10.7567/JJAP.57.06JF03

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title = "Low-autofluorescence fluoropolymer membrane filters for cell filtration",

abstract = "A fluoropolymer membrane filter with through-holes was fabricated by photolithographic patterning and the dry etching method. 380,000 highly packed through-holes, each with a diameter of 7 µm were able to cover a whole area with a diameter of 13 mm. Ethylene tetrafluoroethylene (ETFE) was used as the membrane, which was suitable for the fluorescence detection of rare cells such as circulating tumor cells (CTCs) in human blood. The device fabrication for the size based capture of rare cells in blood such as CTCs is realized in this study.",

author = "Naoto Kihara and Daiki Kuboyama and Daisuke Onoshima and Kenji Ishikawa and Hiromasa Tanaka and Naoya Ozawa and Tetsunari Hase and Ryohei Koguchi and Hiroshi Yukawa and Hidefumi Odaka and Yoshinori Hasegawa and Yoshinobu Baba and Masaru Hori",

note = "Publisher Copyright: {\textcopyright} 2018 The Japan Society of Applied Physics.",

year = "2018",

month = jun,

doi = "10.7567/JJAP.57.06JF03",

language = "English",

volume = "57",

journal = "Japanese Journal of Applied Physics",

issn = "0021-4922",

publisher = "Institute of Physics",

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TY - JOUR

T1 - Low-autofluorescence fluoropolymer membrane filters for cell filtration

AU - Kihara, Naoto

AU - Kuboyama, Daiki

AU - Onoshima, Daisuke

AU - Ishikawa, Kenji

AU - Tanaka, Hiromasa

AU - Ozawa, Naoya

AU - Hase, Tetsunari

AU - Koguchi, Ryohei

AU - Yukawa, Hiroshi

AU - Odaka, Hidefumi

AU - Hasegawa, Yoshinori

AU - Baba, Yoshinobu

AU - Hori, Masaru

PY - 2018/6

Y1 - 2018/6

N2 - A fluoropolymer membrane filter with through-holes was fabricated by photolithographic patterning and the dry etching method. 380,000 highly packed through-holes, each with a diameter of 7 µm were able to cover a whole area with a diameter of 13 mm. Ethylene tetrafluoroethylene (ETFE) was used as the membrane, which was suitable for the fluorescence detection of rare cells such as circulating tumor cells (CTCs) in human blood. The device fabrication for the size based capture of rare cells in blood such as CTCs is realized in this study.

AB - A fluoropolymer membrane filter with through-holes was fabricated by photolithographic patterning and the dry etching method. 380,000 highly packed through-holes, each with a diameter of 7 µm were able to cover a whole area with a diameter of 13 mm. Ethylene tetrafluoroethylene (ETFE) was used as the membrane, which was suitable for the fluorescence detection of rare cells such as circulating tumor cells (CTCs) in human blood. The device fabrication for the size based capture of rare cells in blood such as CTCs is realized in this study.

UR - https://www.scopus.com/pages/publications/85048139421

UR - https://www.scopus.com/pages/publications/85048139421#tab=citedBy

U2 - 10.7567/JJAP.57.06JF03

DO - 10.7567/JJAP.57.06JF03

M3 - Article

AN - SCOPUS:85048139421

SN - 0021-4922

VL - 57

JO - Japanese Journal of Applied Physics

JF - Japanese Journal of Applied Physics

IS - 6

M1 - 06JF03

ER -

Low-autofluorescence fluoropolymer membrane filters for cell filtration

Abstract

All Science Journal Classification (ASJC) codes

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