Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune diseases

Yoshinao Muro, Takahisa Kano, Kazumitsu Sugiura, Masatoshi Hagiwara

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.

Original languageEnglish
Pages (from-to)499-503
Number of pages5
JournalJournal of Autoimmunity
Volume10
Issue number5
DOIs
Publication statusPublished - 10-1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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