TY - JOUR
T1 - Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune diseases
AU - Muro, Yoshinao
AU - Kano, Takahisa
AU - Sugiura, Kazumitsu
AU - Hagiwara, Masatoshi
PY - 1997/10
Y1 - 1997/10
N2 - The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
AB - The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
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U2 - 10.1006/jaut.1997.0157
DO - 10.1006/jaut.1997.0157
M3 - Article
C2 - 9376078
AN - SCOPUS:0030880004
VL - 10
SP - 499
EP - 503
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
SN - 0896-8411
IS - 5
ER -