TY - JOUR
T1 - Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune diseases
AU - Muro, Yoshinao
AU - Kano, Takahisa
AU - Sugiura, Kazumitsu
AU - Hagiwara, Masatoshi
N1 - Funding Information:
We thank Mr T. Onogi (Nagoya University School of Medicine) for DNA sequencing. This work was supported in part by Grants 07770661 and 08770652 from the Ministry of Education in Japan (Dr Muro) and Grants 07282208 and 08457046 from the Ministry of Education in Japan (Dr Hagiwara).
PY - 1997/10
Y1 - 1997/10
N2 - The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
AB - The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
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U2 - 10.1006/jaut.1997.0157
DO - 10.1006/jaut.1997.0157
M3 - Article
C2 - 9376078
AN - SCOPUS:0030880004
SN - 0896-8411
VL - 10
SP - 499
EP - 503
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
IS - 5
ER -