Abstract
The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 499-503 |
| Number of pages | 5 |
| Journal | Journal of Autoimmunity |
| Volume | 10 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 10-1997 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
Cite this
}
Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune diseases. / Muro, Yoshinao; Kano, Takahisa; Sugiura, Kazumitsu; Hagiwara, Masatoshi.
In: Journal of Autoimmunity, Vol. 10, No. 5, 10.1997, p. 499-503.Research output: Contribution to journal › Article
TY - JOUR
T1 - Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune diseases
AU - Muro, Yoshinao
AU - Kano, Takahisa
AU - Sugiura, Kazumitsu
AU - Hagiwara, Masatoshi
PY - 1997/10
Y1 - 1997/10
N2 - The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
AB - The Ki-67 antigen, which is recognized by the monoclonal antibody Ki- 67, is a marker of cell proliferation. During cDNA cloning using sera from a patient with systemic lupus erythematosus, we obtained a positive clone encoding part of Ki-67. We determined the frequency of autoantibodies against Ki-67 in patients with systemic autoimmune diseases. Serum samples from 252 patients with rheumatic diseases were examined by immunoblotting with HeLa nuclear extract and the recombinant N-terminus of the Ki-67 antigen. Autoantibodies against Ki-67 antigen were detected in two out of 76 patients with systemic lupus erythematosus and one out of 90 patients with scleroderma. While, in a previous report, anti-Ki-67 antibodies were frequently targeted by a certain strain of autoimmune mice, our results indicated that Ki-67 was a minor target of autoantibodies among Japanese patients with systemic autoimmune diseases.
UR - http://www.scopus.com/inward/record.url?scp=0030880004&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030880004&partnerID=8YFLogxK
U2 - 10.1006/jaut.1997.0157
DO - 10.1006/jaut.1997.0157
M3 - Article
C2 - 9376078
AN - SCOPUS:0030880004
VL - 10
SP - 499
EP - 503
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
SN - 0896-8411
IS - 5
ER -