Low susceptibility of NC/Nga mice to tumor necrosis factor-α-mediated lethality and hepatocellular damage with d-galactosamine sensitization

Naoki Koide, Akiko Morikawa, Yoshikazu Naiki, Gantsetseg Tumurkhuu, Tomoaki Yoshida, Hiroshi Ikeda, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The susceptibility of NC/Nga mice to tumor necrosis factor (TNF)-α was examined by using sensitization with d-galactosamine (d-GalN). Administration of TNF-α and d-GalN killed none of the NC/Nga mice, whereas it killed all of the BALB/c mice. Treatment with TNF-α and d-GalN caused few hepatic lesions in NC/Nga mice but massive hepatocellular apoptosis in BALB/c mice. Unlike BALB/c mice, there was no elevation in caspase 3 and 8 activities in the livers of NC/Nga mice receiving TNF-α and d-GalN. On the other hand, administration of anti-Fas antibody definitely killed both NC/Nga and BALB/c mice via activation of caspases 3 and 8. Treatment with TNF-α and d-GalN led to translocation of nuclear factor (NF)-κB in NC/Nga and BALB/c mice. However, NF-κB translocation was sustained in NC/Nga mice, although it disappeared in BALB/c mice 7 h after the treatment. NF-κB inhibitors activated caspases 3 and 8, and enhanced TNF-α-mediated lethality in NC/Nga. Taken together, the low susceptibility of NC/Nga mice to TNF-α-mediated lethality was suggested to be responsible for the sustained NF-κB activation.

Original languageEnglish
Pages (from-to)225-232
Number of pages8
JournalClinical Immunology
Volume130
Issue number2
DOIs
Publication statusPublished - 02-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Low susceptibility of NC/Nga mice to tumor necrosis factor-α-mediated lethality and hepatocellular damage with d-galactosamine sensitization'. Together they form a unique fingerprint.

Cite this