Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

J・m Dijkstra, H. Okamoto, M. Ototake, T. Nakanishi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalFish and Shellfish Immunology
Volume11
Issue number2
DOIs
Publication statusPublished - 01-02-2001

Fingerprint

luciferase
Luciferases
glass
injection
antigen
DNA
Glass
antigens
Antigens
vaccination
intramuscular injection
skin (animal)
Fish
Muscle
skin
Skin
muscle
muscles
fish
Kryptopterus

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Dijkstra, J・m ; Okamoto, H. ; Ototake, M. ; Nakanishi, T. / Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus). In: Fish and Shellfish Immunology. 2001 ; Vol. 11, No. 2. pp. 199-202.
@article{3c509d20f6324047a0aaa54d6a801a5e,
title = "Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)",
abstract = "Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.",
author = "J・m Dijkstra and H. Okamoto and M. Ototake and T. Nakanishi",
year = "2001",
month = "2",
day = "1",
doi = "10.1006/fsim.2000.0303",
language = "English",
volume = "11",
pages = "199--202",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Academic Press Inc.",
number = "2",

}

Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus). / Dijkstra, J・m; Okamoto, H.; Ototake, M.; Nakanishi, T.

In: Fish and Shellfish Immunology, Vol. 11, No. 2, 01.02.2001, p. 199-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

AU - Dijkstra, J・m

AU - Okamoto, H.

AU - Ototake, M.

AU - Nakanishi, T.

PY - 2001/2/1

Y1 - 2001/2/1

N2 - Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

AB - Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

UR - http://www.scopus.com/inward/record.url?scp=0035259203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035259203&partnerID=8YFLogxK

U2 - 10.1006/fsim.2000.0303

DO - 10.1006/fsim.2000.0303

M3 - Article

C2 - 11308080

AN - SCOPUS:0035259203

VL - 11

SP - 199

EP - 202

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

IS - 2

ER -