Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

J. M. Dijkstra; H. Okamoto; M. Ototake; T. Nakanishi

doi:10.1006/fsim.2000.0303

Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

J. M. Dijkstra, H. Okamoto, M. Ototake, T. Nakanishi

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Abstract

Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.

Original language	English
Pages (from-to)	199-202
Number of pages	4
Journal	Fish and Shellfish Immunology
Volume	11
Issue number	2
DOIs	https://doi.org/10.1006/fsim.2000.0303
Publication status	Published - 02-2001

All Science Journal Classification (ASJC) codes

Environmental Chemistry
Aquatic Science

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10.1006/fsim.2000.0303

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title = "Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)",

abstract = "Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin. Intramuscular injection of DNA encoding luciferase into these fish induced luciferase expression that was measurable in vivo with a low light video image analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase is not representative of all types of antigen, this study stresses the need for future studies directed to limit the period of antigen expression after DNA vaccination.",

author = "Dijkstra, {J. M.} and H. Okamoto and M. Ototake and T. Nakanishi",

note = "Funding Information: This study was supported by a postdoctoral fellowship for J. M. Dijkstra from the Science and Technology Agency of Japan. We would like to thank Dr K. Araki for supplying us with the pCEP4-luc plasmid and for his helpful comments concerning this manuscript.",

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AU - Okamoto, H.

AU - Ototake, M.

AU - Nakanishi, T.

N1 - Funding Information: This study was supported by a postdoctoral fellowship for J. M. Dijkstra from the Science and Technology Agency of Japan. We would like to thank Dr K. Araki for supplying us with the pCEP4-luc plasmid and for his helpful comments concerning this manuscript.

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