Lymphocyte recruitment via high endothelial venules in lymphoid stroma of Warthin's tumour

Ayumi Ohya, Motohiro Kobayashi, Yasuhiro Sakai, Hiroto Kawashima, Shunsuke Kageyama, Jun Nakayama

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Aims: Warthin's tumour is composed of bilayered oncocytic epithelium and organised lymphoid stroma, which resembles mucosa-associated lymphoid tissue (MALT); however, the histogenesis of the lymphoid stroma is not fully understood. We hypothesised that lymphocytes consisting of the stroma are recruited via high endothelial venules (HEVs) by the mechanism operating in normal lymphocyte homing in secondary lymphoid organs. The aim of this study was to determine immunohistochemically the molecules expressed on these HEVs. Methods: Tissue sections of Warthin's tumour (n=10) were immunostained for vascular addressin-related antigens including peripheral lymph node addressin (PNAd) and mucosal addressin cell adhesion molecule 1 (MAdCAM-1). An L-selectin·IgM chimera in situ binding assay was also carried out. Triple immunostaining for PNAd, CD3, and CD20/CD79α was performed to determine which lymphocyte subsets are closely associated with these HEVs. Results: HEVs in the lymphoid stroma of Warthin's tumour express PNAd, which is detected by MECA-79 as well as recently developed monoclonal antibodies S1 and S2. These HEVs were bound by L-selectin·IgM chimeras in a calciumdependent manner, and numbers of lymphocytes, particularly T cells, attached to these HEVs. Conclusions: The lymphoid stroma of Warthin's tumour is most likely developed by lymphocytes recruited via HEVs.

Original languageEnglish
Pages (from-to)150-154
Number of pages5
Issue number2
Publication statusPublished - 02-2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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