Lymphocytes bursting induced by heparin, dextran sulfate and other polyanions

Keiji Mori, Akira Nakashima, Susumu Sasaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We found that high molecular polyanions (PAs) such as dextran sulfate and heparin burst some damaged or dead lymphocytes in lymphocyte suspension isolated from living bodies, and clarified morphologically the mechanism involved in this. PAs passed through damaged cell membranes and reached the chromatin in the nucleus. Electron micrographs suggested that they then extracted histones from the chromatin and formed complexes with them, and that freed DNA formed a gel binding with the complexes in the nucleus, and the nucleus became swollen and burst due to the gel swelling pressure. To support this conjecture, the nuclei of lymphocytes were stained with propidium iodide and exposed to light to stabilize them. The stabilized nuclei were not swollen after the addition of PAs and the lymphocytes did not burst. The lymphocyte bursting effect of PAs was attributed to their negative charges which could not destroy intact cells. At this point, PAs are different from sodium dodecyl sulfate which destroys intact cells completely.

Original languageEnglish
Pages (from-to)783-787
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume234
Issue number3
DOIs
Publication statusPublished - 29-05-1997

Fingerprint

Dextran Sulfate
Lymphocytes
Heparin
Chromatin
Gels
Propidium
Cell membranes
Sodium Dodecyl Sulfate
Histones
Swelling
Suspensions
Cell Membrane
polyanions
Electrons
Light
Pressure
DNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Lymphocytes bursting induced by heparin, dextran sulfate and other polyanions. / Mori, Keiji; Nakashima, Akira; Sasaki, Susumu.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 3, 29.05.1997, p. 783-787.

Research output: Contribution to journalArticle

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AU - Sasaki, Susumu

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