Macrophage colony stimulating factor stimulates growth progression of the G 1 -phase fraction and induces monocytic differentiation of the G 2 /M-phase fraction in human myeloid leukemia cells

H. Kato, K. Adachi, Motoshi Suzuki, M. Tanimoto, H. Saito

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Abstract

The human myeloid leukemia cell line (NKM-1) proliferates in response to exogenous macrophage colony-stimulating factor (M-CSF) in serum-free medium. This CSF alone, however, does not induce any feature of cell differentiation in NKM-1 cells. We showed that terminal monocytic differentiation is partially observed only when NKM-1 cells are sequentially treated with 1,25-dihydroxyvitamin D 3 and M-CSF, as evaluated by cell morphology, analysis of surface antigens, and phagocytic function. To determine whether there might be an association between the actions of M-CSF and special phases of the cell cycle, we separated D 3 -treated cells into cycle-specific fractions by centrifugal elutriation. G 1 -enriched cells were mostly stimulated for growth progression with M-CSF. On the other hand, G 2 /M-enriched cells were significantly induced into monocytic differentiation. The cell percentage in the G 2 /M phase has clearly correlated with that of terminally differentiated cells by M-CSF. These results suggest that two distinct actions of M-CSF to stimulate cell proliferation and differentiation could work on different phases of the cell cycle: cell growth in the G 1 phase and cell differentiation in the G 2 /M phase.

Original languageEnglish
Pages (from-to)1597-1604
Number of pages8
JournalExperimental Hematology
Volume21
Issue number12
Publication statusPublished - 01-12-1993
Externally publishedYes

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Myeloid Leukemia
Macrophage Colony-Stimulating Factor
Myeloid Cells
Cell Division
Growth
Cell Differentiation
Cell Cycle
Serum-Free Culture Media
Surface Antigens
Cell Proliferation
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

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title = "Macrophage colony stimulating factor stimulates growth progression of the G 1 -phase fraction and induces monocytic differentiation of the G 2 /M-phase fraction in human myeloid leukemia cells",
abstract = "The human myeloid leukemia cell line (NKM-1) proliferates in response to exogenous macrophage colony-stimulating factor (M-CSF) in serum-free medium. This CSF alone, however, does not induce any feature of cell differentiation in NKM-1 cells. We showed that terminal monocytic differentiation is partially observed only when NKM-1 cells are sequentially treated with 1,25-dihydroxyvitamin D 3 and M-CSF, as evaluated by cell morphology, analysis of surface antigens, and phagocytic function. To determine whether there might be an association between the actions of M-CSF and special phases of the cell cycle, we separated D 3 -treated cells into cycle-specific fractions by centrifugal elutriation. G 1 -enriched cells were mostly stimulated for growth progression with M-CSF. On the other hand, G 2 /M-enriched cells were significantly induced into monocytic differentiation. The cell percentage in the G 2 /M phase has clearly correlated with that of terminally differentiated cells by M-CSF. These results suggest that two distinct actions of M-CSF to stimulate cell proliferation and differentiation could work on different phases of the cell cycle: cell growth in the G 1 phase and cell differentiation in the G 2 /M phase.",
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T1 - Macrophage colony stimulating factor stimulates growth progression of the G 1 -phase fraction and induces monocytic differentiation of the G 2 /M-phase fraction in human myeloid leukemia cells

AU - Kato, H.

AU - Adachi, K.

AU - Suzuki, Motoshi

AU - Tanimoto, M.

AU - Saito, H.

PY - 1993/12/1

Y1 - 1993/12/1

N2 - The human myeloid leukemia cell line (NKM-1) proliferates in response to exogenous macrophage colony-stimulating factor (M-CSF) in serum-free medium. This CSF alone, however, does not induce any feature of cell differentiation in NKM-1 cells. We showed that terminal monocytic differentiation is partially observed only when NKM-1 cells are sequentially treated with 1,25-dihydroxyvitamin D 3 and M-CSF, as evaluated by cell morphology, analysis of surface antigens, and phagocytic function. To determine whether there might be an association between the actions of M-CSF and special phases of the cell cycle, we separated D 3 -treated cells into cycle-specific fractions by centrifugal elutriation. G 1 -enriched cells were mostly stimulated for growth progression with M-CSF. On the other hand, G 2 /M-enriched cells were significantly induced into monocytic differentiation. The cell percentage in the G 2 /M phase has clearly correlated with that of terminally differentiated cells by M-CSF. These results suggest that two distinct actions of M-CSF to stimulate cell proliferation and differentiation could work on different phases of the cell cycle: cell growth in the G 1 phase and cell differentiation in the G 2 /M phase.

AB - The human myeloid leukemia cell line (NKM-1) proliferates in response to exogenous macrophage colony-stimulating factor (M-CSF) in serum-free medium. This CSF alone, however, does not induce any feature of cell differentiation in NKM-1 cells. We showed that terminal monocytic differentiation is partially observed only when NKM-1 cells are sequentially treated with 1,25-dihydroxyvitamin D 3 and M-CSF, as evaluated by cell morphology, analysis of surface antigens, and phagocytic function. To determine whether there might be an association between the actions of M-CSF and special phases of the cell cycle, we separated D 3 -treated cells into cycle-specific fractions by centrifugal elutriation. G 1 -enriched cells were mostly stimulated for growth progression with M-CSF. On the other hand, G 2 /M-enriched cells were significantly induced into monocytic differentiation. The cell percentage in the G 2 /M phase has clearly correlated with that of terminally differentiated cells by M-CSF. These results suggest that two distinct actions of M-CSF to stimulate cell proliferation and differentiation could work on different phases of the cell cycle: cell growth in the G 1 phase and cell differentiation in the G 2 /M phase.

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