Magnifying narrow-band imaging of gastric mucosal morphology predicts the H. pylori-related epigenetic field defect

Tomomitsu Tahara, Jumpei Yamazaki, Sayumi Tahara, Masaaki Okubo, Tomohiko Kawamura, Noriyuki Horiguchi, Takamitsu Ishizuka, Mitsuo Nagasaka, Yoshihito Nakagawa, Tomoyuki Shibata, Makoto Kuroda, Naoki Omiya

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Abstract

DNA methylation is associated with "field defect" in the gastric mucosa. To characterize "field defect" morphologically, we examined DNA methylation of non-neoplastic gastric mucosa in relation to their morphology seen by narrow-band imaging (NBI) with magnifying endoscopy. Magnifying NBI of non-neoplastic gastric body was classified as follows: normal-small and round pits with uniform subepithelial capillary networks; type 1-a little enlarged round pits with indistinct subepithelial capillary networks; type 2-remarkably enlarged pits with irregular vessels; and type 3-clearly demarcated oval or tubulovillous pits with bulky coiled or wavy vessels. Methylation of nine candidate genes (MYOD1, SLC16A12, GDNF, IGF2, MIR 124A1, CDH1, PRDM5, RORA and MLF1) were determined by bisulfite pyrosequencing. Infinium HumanMethylation450 array was used to characterize the methylation of >450,000 CpG sites. Mean Z score methylation of nine genes positively correlated with the changes of mucosal patterns from normal to types 1, 2, and 3 (P < 0.0001). Genome-wide analysis showed that development of mucosal patterns correlated with methylation accumulation especially at CpG islands. Genes with promoter CpG islands that were gradually methylated with the development of mucosal patterns significantly enriched the genes involved in zinc-related pathways. The results indicates that gastric mucosal morphology predicts a "field defect" in this tissue type. Accumulation of DNA methylation is associated with "field defect" in the non-neoplastic gastric mucosa. Endoscopic identification of "field defect" has important implications for preventing gastric cancer. Our results suggest that magnifying NBI of gastric mucosal morphology predicts a "field defect" in the gastric mucosa.

Original languageEnglish
Article number03294
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 01-12-2017

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Narrow Band Imaging
Pylorus
Gastric Mucosa
Epigenomics
Methylation
Stomach
DNA Methylation
CpG Islands
Genes
Glial Cell Line-Derived Neurotrophic Factor
Endoscopy
Stomach Neoplasms
Zinc
Genome

All Science Journal Classification (ASJC) codes

  • General

Cite this

Tahara, Tomomitsu ; Yamazaki, Jumpei ; Tahara, Sayumi ; Okubo, Masaaki ; Kawamura, Tomohiko ; Horiguchi, Noriyuki ; Ishizuka, Takamitsu ; Nagasaka, Mitsuo ; Nakagawa, Yoshihito ; Shibata, Tomoyuki ; Kuroda, Makoto ; Omiya, Naoki. / Magnifying narrow-band imaging of gastric mucosal morphology predicts the H. pylori-related epigenetic field defect. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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title = "Magnifying narrow-band imaging of gastric mucosal morphology predicts the H. pylori-related epigenetic field defect",
abstract = "DNA methylation is associated with {"}field defect{"} in the gastric mucosa. To characterize {"}field defect{"} morphologically, we examined DNA methylation of non-neoplastic gastric mucosa in relation to their morphology seen by narrow-band imaging (NBI) with magnifying endoscopy. Magnifying NBI of non-neoplastic gastric body was classified as follows: normal-small and round pits with uniform subepithelial capillary networks; type 1-a little enlarged round pits with indistinct subepithelial capillary networks; type 2-remarkably enlarged pits with irregular vessels; and type 3-clearly demarcated oval or tubulovillous pits with bulky coiled or wavy vessels. Methylation of nine candidate genes (MYOD1, SLC16A12, GDNF, IGF2, MIR 124A1, CDH1, PRDM5, RORA and MLF1) were determined by bisulfite pyrosequencing. Infinium HumanMethylation450 array was used to characterize the methylation of >450,000 CpG sites. Mean Z score methylation of nine genes positively correlated with the changes of mucosal patterns from normal to types 1, 2, and 3 (P < 0.0001). Genome-wide analysis showed that development of mucosal patterns correlated with methylation accumulation especially at CpG islands. Genes with promoter CpG islands that were gradually methylated with the development of mucosal patterns significantly enriched the genes involved in zinc-related pathways. The results indicates that gastric mucosal morphology predicts a {"}field defect{"} in this tissue type. Accumulation of DNA methylation is associated with {"}field defect{"} in the non-neoplastic gastric mucosa. Endoscopic identification of {"}field defect{"} has important implications for preventing gastric cancer. Our results suggest that magnifying NBI of gastric mucosal morphology predicts a {"}field defect{"} in the gastric mucosa.",
author = "Tomomitsu Tahara and Jumpei Yamazaki and Sayumi Tahara and Masaaki Okubo and Tomohiko Kawamura and Noriyuki Horiguchi and Takamitsu Ishizuka and Mitsuo Nagasaka and Yoshihito Nakagawa and Tomoyuki Shibata and Makoto Kuroda and Naoki Omiya",
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Tahara, T, Yamazaki, J, Tahara, S, Okubo, M, Kawamura, T, Horiguchi, N, Ishizuka, T, Nagasaka, M, Nakagawa, Y, Shibata, T, Kuroda, M & Omiya, N 2017, 'Magnifying narrow-band imaging of gastric mucosal morphology predicts the H. pylori-related epigenetic field defect', Scientific reports, vol. 7, no. 1, 03294. https://doi.org/10.1038/s41598-017-03294-8

Magnifying narrow-band imaging of gastric mucosal morphology predicts the H. pylori-related epigenetic field defect. / Tahara, Tomomitsu; Yamazaki, Jumpei; Tahara, Sayumi; Okubo, Masaaki; Kawamura, Tomohiko; Horiguchi, Noriyuki; Ishizuka, Takamitsu; Nagasaka, Mitsuo; Nakagawa, Yoshihito; Shibata, Tomoyuki; Kuroda, Makoto; Omiya, Naoki.

In: Scientific reports, Vol. 7, No. 1, 03294, 01.12.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Magnifying narrow-band imaging of gastric mucosal morphology predicts the H. pylori-related epigenetic field defect

AU - Tahara, Tomomitsu

AU - Yamazaki, Jumpei

AU - Tahara, Sayumi

AU - Okubo, Masaaki

AU - Kawamura, Tomohiko

AU - Horiguchi, Noriyuki

AU - Ishizuka, Takamitsu

AU - Nagasaka, Mitsuo

AU - Nakagawa, Yoshihito

AU - Shibata, Tomoyuki

AU - Kuroda, Makoto

AU - Omiya, Naoki

PY - 2017/12/1

Y1 - 2017/12/1

N2 - DNA methylation is associated with "field defect" in the gastric mucosa. To characterize "field defect" morphologically, we examined DNA methylation of non-neoplastic gastric mucosa in relation to their morphology seen by narrow-band imaging (NBI) with magnifying endoscopy. Magnifying NBI of non-neoplastic gastric body was classified as follows: normal-small and round pits with uniform subepithelial capillary networks; type 1-a little enlarged round pits with indistinct subepithelial capillary networks; type 2-remarkably enlarged pits with irregular vessels; and type 3-clearly demarcated oval or tubulovillous pits with bulky coiled or wavy vessels. Methylation of nine candidate genes (MYOD1, SLC16A12, GDNF, IGF2, MIR 124A1, CDH1, PRDM5, RORA and MLF1) were determined by bisulfite pyrosequencing. Infinium HumanMethylation450 array was used to characterize the methylation of >450,000 CpG sites. Mean Z score methylation of nine genes positively correlated with the changes of mucosal patterns from normal to types 1, 2, and 3 (P < 0.0001). Genome-wide analysis showed that development of mucosal patterns correlated with methylation accumulation especially at CpG islands. Genes with promoter CpG islands that were gradually methylated with the development of mucosal patterns significantly enriched the genes involved in zinc-related pathways. The results indicates that gastric mucosal morphology predicts a "field defect" in this tissue type. Accumulation of DNA methylation is associated with "field defect" in the non-neoplastic gastric mucosa. Endoscopic identification of "field defect" has important implications for preventing gastric cancer. Our results suggest that magnifying NBI of gastric mucosal morphology predicts a "field defect" in the gastric mucosa.

AB - DNA methylation is associated with "field defect" in the gastric mucosa. To characterize "field defect" morphologically, we examined DNA methylation of non-neoplastic gastric mucosa in relation to their morphology seen by narrow-band imaging (NBI) with magnifying endoscopy. Magnifying NBI of non-neoplastic gastric body was classified as follows: normal-small and round pits with uniform subepithelial capillary networks; type 1-a little enlarged round pits with indistinct subepithelial capillary networks; type 2-remarkably enlarged pits with irregular vessels; and type 3-clearly demarcated oval or tubulovillous pits with bulky coiled or wavy vessels. Methylation of nine candidate genes (MYOD1, SLC16A12, GDNF, IGF2, MIR 124A1, CDH1, PRDM5, RORA and MLF1) were determined by bisulfite pyrosequencing. Infinium HumanMethylation450 array was used to characterize the methylation of >450,000 CpG sites. Mean Z score methylation of nine genes positively correlated with the changes of mucosal patterns from normal to types 1, 2, and 3 (P < 0.0001). Genome-wide analysis showed that development of mucosal patterns correlated with methylation accumulation especially at CpG islands. Genes with promoter CpG islands that were gradually methylated with the development of mucosal patterns significantly enriched the genes involved in zinc-related pathways. The results indicates that gastric mucosal morphology predicts a "field defect" in this tissue type. Accumulation of DNA methylation is associated with "field defect" in the non-neoplastic gastric mucosa. Endoscopic identification of "field defect" has important implications for preventing gastric cancer. Our results suggest that magnifying NBI of gastric mucosal morphology predicts a "field defect" in the gastric mucosa.

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