TY - JOUR
T1 - Marked increases in hippocampal neuron indoleamine 2, 3-dioxygenase via IFN-γ-independent pathway following transient global ischemia in mouse
AU - Hoshi, Masato
AU - Saito, Kuniaki
AU - Murakami, Yuki
AU - Taguchi, Ayako
AU - Fujigaki, Hidetsugu
AU - Tanaka, Ryo
AU - Takemura, Masao
AU - Ito, Hiroyasu
AU - Hara, Akira
AU - Seishima, Mitsuru
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/3
Y1 - 2009/3
N2 - Indoleamine 2, 3-dioxygenase (IDO), which catabolizes L-tryptophan (L-TRP) to L-kynurenine (L-KYN), is an immunoregulatory factor that is up-regulated via an interferon-gamma (IFN-γ)-dependent and/or -independent mechanism. In this study, we investigated the localization of IDO and whether induction of IDO expression is an IFN-γ-dependent and/or -independent mechanism in the CNS after cerebral ischemia. The expressions of IDO protein and mRNA were investigated at different time points following cerebral ischemia using immunohistochemistry, immunofluorescence and RT-PCR. Hippocampal neuron IDO mRNA and immunohistochemical staining were significantly up-regulated 72 h after transient global ischemia. Although IFN-γ is a dominant inducer of IDO, hippocampal neuron IDO was clearly up-regulated in IFN-γ KO mice. In summary, this is the first finding that up-regulation of IDO in hippocampal neurons after transient global ischemia occurs via INF-γ-independent mechanisms.
AB - Indoleamine 2, 3-dioxygenase (IDO), which catabolizes L-tryptophan (L-TRP) to L-kynurenine (L-KYN), is an immunoregulatory factor that is up-regulated via an interferon-gamma (IFN-γ)-dependent and/or -independent mechanism. In this study, we investigated the localization of IDO and whether induction of IDO expression is an IFN-γ-dependent and/or -independent mechanism in the CNS after cerebral ischemia. The expressions of IDO protein and mRNA were investigated at different time points following cerebral ischemia using immunohistochemistry, immunofluorescence and RT-PCR. Hippocampal neuron IDO mRNA and immunohistochemical staining were significantly up-regulated 72 h after transient global ischemia. Although IFN-γ is a dominant inducer of IDO, hippocampal neuron IDO was clearly up-regulated in IFN-γ KO mice. In summary, this is the first finding that up-regulation of IDO in hippocampal neurons after transient global ischemia occurs via INF-γ-independent mechanisms.
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U2 - 10.1016/j.neures.2008.12.003
DO - 10.1016/j.neures.2008.12.003
M3 - Article
C2 - 19121343
AN - SCOPUS:60049083839
SN - 0168-0102
VL - 63
SP - 194
EP - 198
JO - Neuroscience Research
JF - Neuroscience Research
IS - 3
ER -