Mass isotopomer analysis of metabolically labeled nucleotide sugars and N-and O-glycans for tracing nucleotide sugar metabolisms

Kazuki Nakajima, Emi Ito, Kazuaki Ohtsubo, Ken Shirato, Rina Takamiya, Shinobu Kitazume, Takashi Angata, Naoyuki Taniguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nucleotide sugars are the donor substrates of various glycosyltransferases, and an important building block in N- and O-glycan biosynthesis. Their intercellular concentrations are regulated by cellular metabolic states including diseases such as cancer and diabetes. To investigate the fate of UDP-GlcNAc, we developed a tracing method for UDP-GlcNAc synthesis and use, and GlcNAc utilization using 13C6-glucose and 13C2-glucosamine, respectively, followed by the analysis of mass isotopomers using LC-MS. Metabolic labeling of cultured cells with 13C6-glucose and the analysis of isotopomers of UDP-HexNAc (UDPGlcNAc plus UDP-GalNAc) and CMP-NeuAc revealed the relative contributions of metabolic pathways leading to UDP-GlcNAc synthesis and use. In pancreatic insulinoma cells, the labeling efficiency of a 13C 6-glucose motif in CMP-NeuAc was lower compared with that in hepatoma cells. Using 13C2-glucosamine, the diversity of the labeling efficiency was observed in each sugar residue of N- and O-glycans on the basis of isotopomer analysis. In the insulinoma cells, the low labeling efficiencies were found for sialic acids as well as tri- and tetra-sialo N-glycans, whereas asialo N-glycans were found to be abundant. Essentially no significant difference in secreted hyaluronic acids was found among hepatoma and insulinoma cell lines. This indicates that metabolic flows are responsible for the low sialylation in the insulinoma cells. Our strategy should be useful for systematically tracing each stage of cellular GlcNAc metabolism.

Original languageEnglish
Pages (from-to)2468-2480
Number of pages13
JournalMolecular and Cellular Proteomics
Volume12
Issue number9
DOIs
Publication statusPublished - 01-09-2013

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Uridine Diphosphate
Insulinoma
Metabolism
Sugars
Polysaccharides
Nucleotides
Labeling
Cytidine Monophosphate
Glucosamine
Glucose
Hepatocellular Carcinoma
Cells
Sialic Acids
Glycosyltransferases
Biosynthesis
Hyaluronic Acid
Medical problems
Metabolic Networks and Pathways
Cultured Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Nakajima, Kazuki ; Ito, Emi ; Ohtsubo, Kazuaki ; Shirato, Ken ; Takamiya, Rina ; Kitazume, Shinobu ; Angata, Takashi ; Taniguchi, Naoyuki. / Mass isotopomer analysis of metabolically labeled nucleotide sugars and N-and O-glycans for tracing nucleotide sugar metabolisms. In: Molecular and Cellular Proteomics. 2013 ; Vol. 12, No. 9. pp. 2468-2480.
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Mass isotopomer analysis of metabolically labeled nucleotide sugars and N-and O-glycans for tracing nucleotide sugar metabolisms. / Nakajima, Kazuki; Ito, Emi; Ohtsubo, Kazuaki; Shirato, Ken; Takamiya, Rina; Kitazume, Shinobu; Angata, Takashi; Taniguchi, Naoyuki.

In: Molecular and Cellular Proteomics, Vol. 12, No. 9, 01.09.2013, p. 2468-2480.

Research output: Contribution to journalArticle

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AU - Nakajima, Kazuki

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AU - Shirato, Ken

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