TY - JOUR
T1 - Maternal molecular hydrogen administration ameliorates rat fetal hippocampal damage caused by in utero ischemia-reperfusion
AU - Mano, Yukio
AU - Kotani, Tomomi
AU - Ito, Mikako
AU - Nagai, Taku
AU - Ichinohashi, Yuko
AU - Yamada, Kiyofumi
AU - Ohno, Kinji
AU - Kikkawa, Fumitaka
AU - Toyokuni, Shinya
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan . Approximately 50%-saturated hydrogen water was a kind gift from Blue Mercury, Inc. (Tokyo, Japan).
PY - 2014/4
Y1 - 2014/4
N2 - Molecular hydrogen (H2) scavenges hydroxyl radicals. Recently, H2 has been reported to prevent a variety of diseases associated with oxidative stress in model systems and in humans. Here, we studied the effects of H2 on rat fetal hippocampal damage caused by ischemia and reperfusion (IR) on day 16 of pregnancy with the transient occlusion of the bilateral utero-ovarian arteries. Starting 2 days before the operation, we provided the mothers with hydrogen-saturated water ad libitum until vaginal delivery. We observed a significant increase in the concentration of H 2 in the placenta after the oral administration of hydrogen-saturated water to the mothers, with less placental oxidative damage after IR in the presence of H2. Neonatal growth retardation was observed in the IR group, which was alleviated by the H2 administration. We analyzed the neuronal cell damage in the CA1 and CA3 areas of the hippocampus at day 7 after birth by immunohistochemical analysis of the 8-oxo-7,8-dihydro-2.
AB - Molecular hydrogen (H2) scavenges hydroxyl radicals. Recently, H2 has been reported to prevent a variety of diseases associated with oxidative stress in model systems and in humans. Here, we studied the effects of H2 on rat fetal hippocampal damage caused by ischemia and reperfusion (IR) on day 16 of pregnancy with the transient occlusion of the bilateral utero-ovarian arteries. Starting 2 days before the operation, we provided the mothers with hydrogen-saturated water ad libitum until vaginal delivery. We observed a significant increase in the concentration of H 2 in the placenta after the oral administration of hydrogen-saturated water to the mothers, with less placental oxidative damage after IR in the presence of H2. Neonatal growth retardation was observed in the IR group, which was alleviated by the H2 administration. We analyzed the neuronal cell damage in the CA1 and CA3 areas of the hippocampus at day 7 after birth by immunohistochemical analysis of the 8-oxo-7,8-dihydro-2.
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U2 - 10.1016/j.freeradbiomed.2014.01.037
DO - 10.1016/j.freeradbiomed.2014.01.037
M3 - Article
C2 - 24509162
AN - SCOPUS:84896806726
SN - 0891-5849
VL - 69
SP - 324
EP - 330
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
ER -