Abstract
To assess its possible role in the pathophysiology of intervertebral disc degeneration, we investigated the production of matrix metalloproteinase-3 (MMP3) using human intervertebral disc explant culture. Five normal and 10 degenerated disc specimens were used. The levels of MMP-3 released in the medium were measured with use of an enzyme immunoassay. The results showed that the level of MMP-3 in the degenerated group (0.57 μg/ml/mg wet weight; n = 10) was significantly higher than that of the control group (0.29 μg/ml/mg wet weight; n = 5) (p < 0.05). Immunostaining of MMP-3 revealed that the ratio of positive staining cells in the degenerated group was greater than that of the control group. These observations suggest that MMP-3 produced by human intervertebral disc may be involved in the intervertebral disc degeneration, particularly in the initiation of matrix degradation of intervertebral disc.
| Original language | English |
|---|---|
| Pages (from-to) | 493-498 |
| Number of pages | 6 |
| Journal | Journal of Spinal Disorders |
| Volume | 10 |
| Issue number | 6 |
| Publication status | Published - 01-12-1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Surgery
- Clinical Neurology
Cite this
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Matrix metalloproteinase-3 production by human degenerated intervertebral disc. / Nemoto, Osamu; Yamagishi, Masaaki; Yamada, Harumoto; Kikuchi, Toshiyuki; Takaishi, Hironari.
In: Journal of Spinal Disorders, Vol. 10, No. 6, 01.12.1997, p. 493-498.Research output: Contribution to journal › Article
TY - JOUR
T1 - Matrix metalloproteinase-3 production by human degenerated intervertebral disc
AU - Nemoto, Osamu
AU - Yamagishi, Masaaki
AU - Yamada, Harumoto
AU - Kikuchi, Toshiyuki
AU - Takaishi, Hironari
PY - 1997/12/1
Y1 - 1997/12/1
N2 - To assess its possible role in the pathophysiology of intervertebral disc degeneration, we investigated the production of matrix metalloproteinase-3 (MMP3) using human intervertebral disc explant culture. Five normal and 10 degenerated disc specimens were used. The levels of MMP-3 released in the medium were measured with use of an enzyme immunoassay. The results showed that the level of MMP-3 in the degenerated group (0.57 μg/ml/mg wet weight; n = 10) was significantly higher than that of the control group (0.29 μg/ml/mg wet weight; n = 5) (p < 0.05). Immunostaining of MMP-3 revealed that the ratio of positive staining cells in the degenerated group was greater than that of the control group. These observations suggest that MMP-3 produced by human intervertebral disc may be involved in the intervertebral disc degeneration, particularly in the initiation of matrix degradation of intervertebral disc.
AB - To assess its possible role in the pathophysiology of intervertebral disc degeneration, we investigated the production of matrix metalloproteinase-3 (MMP3) using human intervertebral disc explant culture. Five normal and 10 degenerated disc specimens were used. The levels of MMP-3 released in the medium were measured with use of an enzyme immunoassay. The results showed that the level of MMP-3 in the degenerated group (0.57 μg/ml/mg wet weight; n = 10) was significantly higher than that of the control group (0.29 μg/ml/mg wet weight; n = 5) (p < 0.05). Immunostaining of MMP-3 revealed that the ratio of positive staining cells in the degenerated group was greater than that of the control group. These observations suggest that MMP-3 produced by human intervertebral disc may be involved in the intervertebral disc degeneration, particularly in the initiation of matrix degradation of intervertebral disc.
UR - http://www.scopus.com/inward/record.url?scp=0031452651&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031452651&partnerID=8YFLogxK
M3 - Article
VL - 10
SP - 493
EP - 498
JO - Journal of Spinal Disorders
JF - Journal of Spinal Disorders
SN - 1536-0652
IS - 6
ER -