Matrix metalloproteinase-3 production by human degenerated intervertebral disc

Osamu Nemoto, Masaaki Yamagishi, Harumoto Yamada, Toshiyuki Kikuchi, Hironari Takaishi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

To assess its possible role in the pathophysiology of intervertebral disc degeneration, we investigated the production of matrix metalloproteinase-3 (MMP3) using human intervertebral disc explant culture. Five normal and 10 degenerated disc specimens were used. The levels of MMP-3 released in the medium were measured with use of an enzyme immunoassay. The results showed that the level of MMP-3 in the degenerated group (0.57 μg/ml/mg wet weight; n = 10) was significantly higher than that of the control group (0.29 μg/ml/mg wet weight; n = 5) (p < 0.05). Immunostaining of MMP-3 revealed that the ratio of positive staining cells in the degenerated group was greater than that of the control group. These observations suggest that MMP-3 produced by human intervertebral disc may be involved in the intervertebral disc degeneration, particularly in the initiation of matrix degradation of intervertebral disc.

Original languageEnglish
Pages (from-to)493-498
Number of pages6
JournalJournal of Spinal Disorders
Volume10
Issue number6
Publication statusPublished - 01-12-1997
Externally publishedYes

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Matrix Metalloproteinase 3
Intervertebral Disc
Matrix Metalloproteinases
Intervertebral Disc Degeneration
Weights and Measures
Control Groups
Immunoenzyme Techniques
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Cite this

Nemoto, O., Yamagishi, M., Yamada, H., Kikuchi, T., & Takaishi, H. (1997). Matrix metalloproteinase-3 production by human degenerated intervertebral disc. Journal of Spinal Disorders, 10(6), 493-498.
Nemoto, Osamu ; Yamagishi, Masaaki ; Yamada, Harumoto ; Kikuchi, Toshiyuki ; Takaishi, Hironari. / Matrix metalloproteinase-3 production by human degenerated intervertebral disc. In: Journal of Spinal Disorders. 1997 ; Vol. 10, No. 6. pp. 493-498.
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Nemoto, O, Yamagishi, M, Yamada, H, Kikuchi, T & Takaishi, H 1997, 'Matrix metalloproteinase-3 production by human degenerated intervertebral disc', Journal of Spinal Disorders, vol. 10, no. 6, pp. 493-498.

Matrix metalloproteinase-3 production by human degenerated intervertebral disc. / Nemoto, Osamu; Yamagishi, Masaaki; Yamada, Harumoto; Kikuchi, Toshiyuki; Takaishi, Hironari.

In: Journal of Spinal Disorders, Vol. 10, No. 6, 01.12.1997, p. 493-498.

Research output: Contribution to journalArticle

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