Matrix metalloproteinase-3 production by human degenerated intervertebral disc

Osamu Nemoto, Masaaki Yamagishi, Harumoto Yamada, Toshiyuki Kikuchi, Hironari Takaishi

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50 Citations (Scopus)

Abstract

To assess its possible role in the pathophysiology of intervertebral disc degeneration, we investigated the production of matrix metalloproteinase-3 (MMP3) using human intervertebral disc explant culture. Five normal and 10 degenerated disc specimens were used. The levels of MMP-3 released in the medium were measured with use of an enzyme immunoassay. The results showed that the level of MMP-3 in the degenerated group (0.57 μg/ml/mg wet weight; n = 10) was significantly higher than that of the control group (0.29 μg/ml/mg wet weight; n = 5) (p < 0.05). Immunostaining of MMP-3 revealed that the ratio of positive staining cells in the degenerated group was greater than that of the control group. These observations suggest that MMP-3 produced by human intervertebral disc may be involved in the intervertebral disc degeneration, particularly in the initiation of matrix degradation of intervertebral disc.

Original languageEnglish
Pages (from-to)493-498
Number of pages6
JournalJournal of Spinal Disorders
Volume10
Issue number6
DOIs
Publication statusPublished - 01-01-1997

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

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