Matrix metalloproteinase-9 contributes to kindled seizure development in pentylenetetrazole-treated mice by converting pro-BDNF to mature BDNF in the hippocampus

Hiroyuki Mizoguchi, Junya Nakade, Masaki Tachibana, Daisuke Ibi, Eiichi Someya, Hiroyuki Koike, Hiroyuki Kamei, Toshitaka Nabeshima, Shigeyoshi Itohara, Kazuhiro Takuma, Makoto Sawada, Jun Sato, Kiyofumi Yamada

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Abstract

Recurrent seizure activity has been shown to induce a variety of permanent structural changes in the brain. Matrix metalloproteinases (MMPs) function to promote neuronal plasticity, primarily through cleavage of extracellular matrix proteins. Here, we investigated the role ofMMP-9in the development of pentylenetetrazole (PTZ)-induced kindled seizure in mice. Repeated treatment with PTZ (40 mg/kg) produced kindled seizure, which was accompanied by enhanced MMP-9 activity and expression in the hippocampus. No change in MMP-9 activity was observed in the hippocampi of mice with generalized tonic seizure following single administration of PTZ (60 mg/kg). MMP-9 colocalized with the neuronal marker NeuN and the glial marker GFAP in the dentate gyrus of the kindled mouse hippocampus. Coadministration of diazepam or MK-801 with PTZ inhibited the development of kindling and the increased MMP-9 levels in the hippocampus. Marked suppression of kindled seizure progression in response to repeated PTZ treatment was observed in MMP-9(-/-) mice compared with wild-type mice, an observation that was accompanied by decreased hippocampal levels of mature brain-derived neurotrophic factor. Microinjecting the BDNF scavenger TrkB-Fc into the right ventricle before each PTZ treatment significantly suppressed the development of kindling in wild-type mice, whereas no effect was observed in MMP-9(-/-) mice. On the other hand, bilateral injections of pro-BDNF into the hippocampal dentate gyrus significantly enhanced kindling in wild-type mice but not MMP-9(-/-) mice. These findings suggest thatMMP-9is involved in the progression of behavioral phenotypes in kindled mice because of conversion of pro-BDNF to mature BDNF in the hippocampus.

Original languageEnglish
Pages (from-to)12963-12971
Number of pages9
JournalJournal of Neuroscience
Volume31
Issue number36
DOIs
Publication statusPublished - 07-09-2011
Externally publishedYes

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Pentylenetetrazole
Brain-Derived Neurotrophic Factor
Matrix Metalloproteinase 9
Hippocampus
Seizures
Dentate Gyrus
brain-derived neurotrophic factor precursor
Parahippocampal Gyrus
Neuronal Plasticity
Dizocilpine Maleate
Extracellular Matrix Proteins
Diazepam
Matrix Metalloproteinases
Neuroglia
Heart Ventricles
Therapeutics
Observation
Phenotype

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Mizoguchi, Hiroyuki ; Nakade, Junya ; Tachibana, Masaki ; Ibi, Daisuke ; Someya, Eiichi ; Koike, Hiroyuki ; Kamei, Hiroyuki ; Nabeshima, Toshitaka ; Itohara, Shigeyoshi ; Takuma, Kazuhiro ; Sawada, Makoto ; Sato, Jun ; Yamada, Kiyofumi. / Matrix metalloproteinase-9 contributes to kindled seizure development in pentylenetetrazole-treated mice by converting pro-BDNF to mature BDNF in the hippocampus. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 36. pp. 12963-12971.
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abstract = "Recurrent seizure activity has been shown to induce a variety of permanent structural changes in the brain. Matrix metalloproteinases (MMPs) function to promote neuronal plasticity, primarily through cleavage of extracellular matrix proteins. Here, we investigated the role ofMMP-9in the development of pentylenetetrazole (PTZ)-induced kindled seizure in mice. Repeated treatment with PTZ (40 mg/kg) produced kindled seizure, which was accompanied by enhanced MMP-9 activity and expression in the hippocampus. No change in MMP-9 activity was observed in the hippocampi of mice with generalized tonic seizure following single administration of PTZ (60 mg/kg). MMP-9 colocalized with the neuronal marker NeuN and the glial marker GFAP in the dentate gyrus of the kindled mouse hippocampus. Coadministration of diazepam or MK-801 with PTZ inhibited the development of kindling and the increased MMP-9 levels in the hippocampus. Marked suppression of kindled seizure progression in response to repeated PTZ treatment was observed in MMP-9(-/-) mice compared with wild-type mice, an observation that was accompanied by decreased hippocampal levels of mature brain-derived neurotrophic factor. Microinjecting the BDNF scavenger TrkB-Fc into the right ventricle before each PTZ treatment significantly suppressed the development of kindling in wild-type mice, whereas no effect was observed in MMP-9(-/-) mice. On the other hand, bilateral injections of pro-BDNF into the hippocampal dentate gyrus significantly enhanced kindling in wild-type mice but not MMP-9(-/-) mice. These findings suggest thatMMP-9is involved in the progression of behavioral phenotypes in kindled mice because of conversion of pro-BDNF to mature BDNF in the hippocampus.",
author = "Hiroyuki Mizoguchi and Junya Nakade and Masaki Tachibana and Daisuke Ibi and Eiichi Someya and Hiroyuki Koike and Hiroyuki Kamei and Toshitaka Nabeshima and Shigeyoshi Itohara and Kazuhiro Takuma and Makoto Sawada and Jun Sato and Kiyofumi Yamada",
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Mizoguchi, H, Nakade, J, Tachibana, M, Ibi, D, Someya, E, Koike, H, Kamei, H, Nabeshima, T, Itohara, S, Takuma, K, Sawada, M, Sato, J & Yamada, K 2011, 'Matrix metalloproteinase-9 contributes to kindled seizure development in pentylenetetrazole-treated mice by converting pro-BDNF to mature BDNF in the hippocampus', Journal of Neuroscience, vol. 31, no. 36, pp. 12963-12971. https://doi.org/10.1523/JNEUROSCI.3118-11.2011

Matrix metalloproteinase-9 contributes to kindled seizure development in pentylenetetrazole-treated mice by converting pro-BDNF to mature BDNF in the hippocampus. / Mizoguchi, Hiroyuki; Nakade, Junya; Tachibana, Masaki; Ibi, Daisuke; Someya, Eiichi; Koike, Hiroyuki; Kamei, Hiroyuki; Nabeshima, Toshitaka; Itohara, Shigeyoshi; Takuma, Kazuhiro; Sawada, Makoto; Sato, Jun; Yamada, Kiyofumi.

In: Journal of Neuroscience, Vol. 31, No. 36, 07.09.2011, p. 12963-12971.

Research output: Contribution to journalArticle

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AU - Mizoguchi, Hiroyuki

AU - Nakade, Junya

AU - Tachibana, Masaki

AU - Ibi, Daisuke

AU - Someya, Eiichi

AU - Koike, Hiroyuki

AU - Kamei, Hiroyuki

AU - Nabeshima, Toshitaka

AU - Itohara, Shigeyoshi

AU - Takuma, Kazuhiro

AU - Sawada, Makoto

AU - Sato, Jun

AU - Yamada, Kiyofumi

PY - 2011/9/7

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