Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration

The first clinical trial in 130 cases

Hiroya Yamada, Toru Takano, Minoru Kihara, Mitsuyoshi Hirokawa, Hiroshi Yoshida, Mikio Watanabe, Yoshinori Iwatani, Yoh Hidaka, Akira Miyauchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Measurement of gene expression levels in thyroid tumor cells in aspirates was difficult because it is interfered with peripheral blood cells or infiltrating lymphocytes. In this study, we established a novel method to separate thyroid tumor cells from blood cells efficiently with mesh filtration. The expression level of trefoil factor 3 (TFF3) mRNA was estimated using LGALS3 mRNA as an internal control (T/G ratio) in 148 preoperative thyroid aspirates. Intra-assay coefficients of variation (CV) of T/G ratio for high, moderate, and low samples were 6.5%, 2.5%, and 9.7%, respectively, and inter-assay CV for high, moderate, and low samples were 27.7%, 21.9%, and 38.2%, respectively. Nondiagnostic samples in terms of T/G ratio and cytology were 12.2% and 16.9%, respectively. We observed no interference with the data by contaminating blood cells. Among these patients, 12 patients received more than two repeated aspirations. We did not observe a marked day-to-day variation except in two cases. All 13 preoperative aspirates diagnosed as malignant by cytology showed an extremely low T/G ratio, whereas 93 aspirates diagnosed as benign by cytology showed extremely varied T/G ratios and 21.5% of them showed a T/G ratio below the cut-off value. Eleven cases underwent surgery. All nodules showing a low T/G ratio were diagnosed as papillary carcinoma by pathological diagnosis. However, one nodule diagnosed as follicular adenoma after surgery showed a high T/G ratio. Our present method may be a promising preoperative test for measuring mRNAs in thyroid aspirates.

Original languageEnglish
Pages (from-to)621-630
Number of pages10
JournalEndocrine Journal
Volume59
Issue number7
DOIs
Publication statusPublished - 06-09-2012

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Thyroid Nodule
Thyroid Gland
Clinical Trials
Cell Biology
Blood Cells
Messenger RNA
Galectin 3
Papillary Carcinoma
Adenoma
Neoplasms
Lymphocytes
Gene Expression
Trefoil Factor-3

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Yamada, Hiroya ; Takano, Toru ; Kihara, Minoru ; Hirokawa, Mitsuyoshi ; Yoshida, Hiroshi ; Watanabe, Mikio ; Iwatani, Yoshinori ; Hidaka, Yoh ; Miyauchi, Akira. / Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration : The first clinical trial in 130 cases. In: Endocrine Journal. 2012 ; Vol. 59, No. 7. pp. 621-630.
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title = "Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration: The first clinical trial in 130 cases",
abstract = "Measurement of gene expression levels in thyroid tumor cells in aspirates was difficult because it is interfered with peripheral blood cells or infiltrating lymphocytes. In this study, we established a novel method to separate thyroid tumor cells from blood cells efficiently with mesh filtration. The expression level of trefoil factor 3 (TFF3) mRNA was estimated using LGALS3 mRNA as an internal control (T/G ratio) in 148 preoperative thyroid aspirates. Intra-assay coefficients of variation (CV) of T/G ratio for high, moderate, and low samples were 6.5{\%}, 2.5{\%}, and 9.7{\%}, respectively, and inter-assay CV for high, moderate, and low samples were 27.7{\%}, 21.9{\%}, and 38.2{\%}, respectively. Nondiagnostic samples in terms of T/G ratio and cytology were 12.2{\%} and 16.9{\%}, respectively. We observed no interference with the data by contaminating blood cells. Among these patients, 12 patients received more than two repeated aspirations. We did not observe a marked day-to-day variation except in two cases. All 13 preoperative aspirates diagnosed as malignant by cytology showed an extremely low T/G ratio, whereas 93 aspirates diagnosed as benign by cytology showed extremely varied T/G ratios and 21.5{\%} of them showed a T/G ratio below the cut-off value. Eleven cases underwent surgery. All nodules showing a low T/G ratio were diagnosed as papillary carcinoma by pathological diagnosis. However, one nodule diagnosed as follicular adenoma after surgery showed a high T/G ratio. Our present method may be a promising preoperative test for measuring mRNAs in thyroid aspirates.",
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Yamada, H, Takano, T, Kihara, M, Hirokawa, M, Yoshida, H, Watanabe, M, Iwatani, Y, Hidaka, Y & Miyauchi, A 2012, 'Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration: The first clinical trial in 130 cases', Endocrine Journal, vol. 59, no. 7, pp. 621-630. https://doi.org/10.1507/endocrj.EJ12-0077

Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration : The first clinical trial in 130 cases. / Yamada, Hiroya; Takano, Toru; Kihara, Minoru; Hirokawa, Mitsuyoshi; Yoshida, Hiroshi; Watanabe, Mikio; Iwatani, Yoshinori; Hidaka, Yoh; Miyauchi, Akira.

In: Endocrine Journal, Vol. 59, No. 7, 06.09.2012, p. 621-630.

Research output: Contribution to journalArticle

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AU - Hirokawa, Mitsuyoshi

AU - Yoshida, Hiroshi

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