Measurement of the transport activities of bile salt export pump using LC-MS

Kana Yamaguchi, Tsuyoshi Murai, Hikaru Yabuuchi, Takao Kurosawa

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The high performance liquid chromatography-electrospray ionization-mass spectrometry method has been applied to the measurement of bile acid transport activities in membrane vesicles obtained from a human bile salt export pump expressing Sf9 cells. The amounts of bile acids transported using the human bile salt export pump expressing Sf9 cells were determined using liquid chromatography-electrospray ionization-mass spectrometry method and the values of the kinetic parameters were determined to be comparable with those obtained using radioisotope-labeled substrates. The developed method was highly useful for the measurements of bile acid transport activities. 2009

Original languageEnglish
Pages (from-to)1155-1158
Number of pages4
JournalAnalytical Sciences
Volume25
Issue number9
DOIs
Publication statusPublished - 09-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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