Measurement of transport activities of bile acids in human multidrug resistance-associated protein 3 using liquid chromatography-tandem mass spectrometry

Kana Yamaguchi, Tsuyoshi murai, hikaru Yabuuchi, Takao Kurosawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A method has been developed for the measurement of transport activities in membrane vesicles obtained from human multidrug resistance-associated protein 3-expressing Sf9 cells for 1β-hydroxy-, 6α-hydroxy- and unsaturated bile acids by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Calibration curves for the bile acids were linear over the range of 10 to 2000 pmol/mL, and the detection limit was less than 2 pmol/mL for all bile acids using selected reaction monitoring analysis. The method was applied to measurements of adenosine triphosphate-dependent transport activities of the membrane vesicles for the above-mentioned hydroxylated and unsaturated bile acids. The present study demonstrated that the human multidrug resistance-associated protein 3 vesicles accepted 1β-, 6α-hydroxylated and unsaturated bile acids along with common bile acids, such as glycocholic acid and taurolithocholic acid 3-sulfate. The developed method is useful for measurements of bile acid transport activities. 2010

Original languageEnglish
Pages (from-to)317-323
Number of pages7
JournalAnalytical Sciences
Volume26
Issue number3
DOIs
Publication statusPublished - 03-2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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