Mechanical stretch enhances IL-8 production in pulmonary microvascular endothelial cells

Mai Iwaki, Satoru Ito, Masataka Morioka, Susumu Iwata, Yasushi Numaguchi, Masakazu Ishii, Masashi Kondo, Hiroaki Kume, Keiji Naruse, Masahiro Sokabe, Yoshinori Hasegawa

Research output: Contribution to journalArticle

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Abstract

In patients with acute respiratory distress syndrome, mechanical over-distension of the lung by a large tidal volume causes further damage and inflammation, called ventilator-induced lung injury (VILI), however, it is unclear how mechanical stretch affects the cellular functions or morphology in human pulmonary microvascular endothelial cells (HPMVECs). IL-8 has been proposed to play an important role in the progression of VILI by activating neutrophils. We demonstrated that HPMVECs exposed to cyclic uni-axial stretch produce IL-8 protein with p38 activation in strain- and time-dependent manners. The IL-8 synthesis was not regulated by other signal transduction pathways such as ERK1/2, JNK, or stretch-activated Ca2+ channels. Moreover, cyclic stretch enhanced IL-6 and monocyte chemoattractant protein-1 production and reoriented cell perpendicularly to the stretch axis accompanied by actin polymerization. Taken together, IL-8 production by HPMVECs due to excessive mechanical stretch may activate neutrophilic inflammation, which leads to VILI.

Original languageEnglish
Pages (from-to)531-536
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume389
Issue number3
DOIs
Publication statusPublished - 01-01-2009

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Ventilator-Induced Lung Injury
Endothelial cells
Interleukin-8
Endothelial Cells
Lung
Inflammation
Signal transduction
Chemokine CCL2
Tidal Volume
Adult Respiratory Distress Syndrome
Polymerization
Actins
Interleukin-6
Signal Transduction
Neutrophils
Chemical activation
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Iwaki, Mai ; Ito, Satoru ; Morioka, Masataka ; Iwata, Susumu ; Numaguchi, Yasushi ; Ishii, Masakazu ; Kondo, Masashi ; Kume, Hiroaki ; Naruse, Keiji ; Sokabe, Masahiro ; Hasegawa, Yoshinori. / Mechanical stretch enhances IL-8 production in pulmonary microvascular endothelial cells. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 389, No. 3. pp. 531-536.
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Iwaki, M, Ito, S, Morioka, M, Iwata, S, Numaguchi, Y, Ishii, M, Kondo, M, Kume, H, Naruse, K, Sokabe, M & Hasegawa, Y 2009, 'Mechanical stretch enhances IL-8 production in pulmonary microvascular endothelial cells', Biochemical and Biophysical Research Communications, vol. 389, no. 3, pp. 531-536. https://doi.org/10.1016/j.bbrc.2009.09.020

Mechanical stretch enhances IL-8 production in pulmonary microvascular endothelial cells. / Iwaki, Mai; Ito, Satoru; Morioka, Masataka; Iwata, Susumu; Numaguchi, Yasushi; Ishii, Masakazu; Kondo, Masashi; Kume, Hiroaki; Naruse, Keiji; Sokabe, Masahiro; Hasegawa, Yoshinori.

In: Biochemical and Biophysical Research Communications, Vol. 389, No. 3, 01.01.2009, p. 531-536.

Research output: Contribution to journalArticle

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T1 - Mechanical stretch enhances IL-8 production in pulmonary microvascular endothelial cells

AU - Iwaki, Mai

AU - Ito, Satoru

AU - Morioka, Masataka

AU - Iwata, Susumu

AU - Numaguchi, Yasushi

AU - Ishii, Masakazu

AU - Kondo, Masashi

AU - Kume, Hiroaki

AU - Naruse, Keiji

AU - Sokabe, Masahiro

AU - Hasegawa, Yoshinori

PY - 2009/1/1

Y1 - 2009/1/1

N2 - In patients with acute respiratory distress syndrome, mechanical over-distension of the lung by a large tidal volume causes further damage and inflammation, called ventilator-induced lung injury (VILI), however, it is unclear how mechanical stretch affects the cellular functions or morphology in human pulmonary microvascular endothelial cells (HPMVECs). IL-8 has been proposed to play an important role in the progression of VILI by activating neutrophils. We demonstrated that HPMVECs exposed to cyclic uni-axial stretch produce IL-8 protein with p38 activation in strain- and time-dependent manners. The IL-8 synthesis was not regulated by other signal transduction pathways such as ERK1/2, JNK, or stretch-activated Ca2+ channels. Moreover, cyclic stretch enhanced IL-6 and monocyte chemoattractant protein-1 production and reoriented cell perpendicularly to the stretch axis accompanied by actin polymerization. Taken together, IL-8 production by HPMVECs due to excessive mechanical stretch may activate neutrophilic inflammation, which leads to VILI.

AB - In patients with acute respiratory distress syndrome, mechanical over-distension of the lung by a large tidal volume causes further damage and inflammation, called ventilator-induced lung injury (VILI), however, it is unclear how mechanical stretch affects the cellular functions or morphology in human pulmonary microvascular endothelial cells (HPMVECs). IL-8 has been proposed to play an important role in the progression of VILI by activating neutrophils. We demonstrated that HPMVECs exposed to cyclic uni-axial stretch produce IL-8 protein with p38 activation in strain- and time-dependent manners. The IL-8 synthesis was not regulated by other signal transduction pathways such as ERK1/2, JNK, or stretch-activated Ca2+ channels. Moreover, cyclic stretch enhanced IL-6 and monocyte chemoattractant protein-1 production and reoriented cell perpendicularly to the stretch axis accompanied by actin polymerization. Taken together, IL-8 production by HPMVECs due to excessive mechanical stretch may activate neutrophilic inflammation, which leads to VILI.

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