Mechanical white blood cell damage in rotary blood pumps

Yoshiyuki Takami, Shingo Yamane, Kenzo Makinouchi, Julie Glueck, Yukihiko Nosé

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mechanical trauma of white blood cells (WBC) due to the operation of a rotary blood pump was examined, using a simple method of trypan blue dye exclusion test for a cell viability measurement. The degree of WBC trauma was investigated using a roller pump (RP) and 3 commercially available centrifugal pumps (Bio-Medicus [BP], Capiox [CP], Nikkiso [NK]), and compared with the red blood cell (RBC) trauma. Each pump was operated 3 times at a flow rate of 5 L/min against the total pressure head of 350 mm Hg for 6 h in a mock circuit with 400 ml of fresh bovine blood. Blood was sampled at 2 h intervals measuring plasma free hemoglobin concentration and the percentage of damaged WBC in the trypan blue dye exclusion test. Each pump demonstrated a linear increase in the degree of WBC trauma, and there were differences among the tested pumps (RP > BP > CP > NK). These findings were similar to those of the free hemoglobin measurements. To compare the degree of RBC and WBC trauma, the probability (γ,ω) of RBC and WBC to be damaged was calculated, respectively. γ = ΔD(RBC)/ΔN, ω = ΔD(WBC)/ΔN where D(RBC) and D(WBC) are the ratios of the damaged RBC and WBC, respectively, and N is the passing number defined as Qt/V (Q, flow rate; t, time; V, circulating volume). The data of this study demonstrated that the ω value was approximately 20 times or more greater than the γ equally in all the tested pumps. This suggests that a WBC is more vulnerable to mechanical damage by a rotary blood pump than a RBC.

Original languageEnglish
Pages (from-to)138-142
Number of pages5
JournalArtificial Organs
Volume21
Issue number2
DOIs
Publication statusPublished - 1997

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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