Mechanism of atopic cataract caused by eosinophil granule major basic protein

Naoki Yamamoto, Noriko Hiramatsu, Sumito Isogai, Masashi Kondo, Kazuyoshi Imaizumi, Masayuki Horiguchi

Research output: Contribution to journalArticle

Abstract

Atopic cataracts develop under the ages of 40 years, after which visual acuity rapidly declines. However, the mechanism underlying the development of atopic cataracts is not yet clear. We focused on the eosinophil granule major basic protein (MBP), which was detected in the aqueous humor of atopic cataracts previously, and which was cytotoxic. Specifically, we investigated its origin in this fluid and its effects on lens epithelial cells (LECs). MBP immunostaining was positive in atopic cataract-derived LECs, but negative in age-related cataract-derived LECs. MBP mRNA was not detected in either type of cataract, but protein was detected in the aqueous humor. Furthermore, the flare values associated with atopic cataracts were higher than those with age-related cataracts. When MBP was purified from eosinophils or recombinant MBP was added to LEC culture medium, cell viability decreased in a concentration-dependent manner, but an MBP antibody neutralized the cytotoxic effect of this protein towards these cells. These results were consistent with the flow of MBP into the aqueous humor from the blood due to a compromised blood–aqueous barrier. Thus, MBP could further penetrate the lens capsule and adhere to LECs, resulting in decreased cell viability and the development of atopic cataracts.

Original languageEnglish
JournalMedical Molecular Morphology
DOIs
Publication statusAccepted/In press - 01-01-2019

Fingerprint

Eosinophil Major Basic Protein
Cataract
Lenses
Epithelial Cells
Proteins
Aqueous Humor
Cell Survival
Eosinophils
Visual Acuity
Capsules
Culture Media
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

Cite this

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title = "Mechanism of atopic cataract caused by eosinophil granule major basic protein",
abstract = "Atopic cataracts develop under the ages of 40 years, after which visual acuity rapidly declines. However, the mechanism underlying the development of atopic cataracts is not yet clear. We focused on the eosinophil granule major basic protein (MBP), which was detected in the aqueous humor of atopic cataracts previously, and which was cytotoxic. Specifically, we investigated its origin in this fluid and its effects on lens epithelial cells (LECs). MBP immunostaining was positive in atopic cataract-derived LECs, but negative in age-related cataract-derived LECs. MBP mRNA was not detected in either type of cataract, but protein was detected in the aqueous humor. Furthermore, the flare values associated with atopic cataracts were higher than those with age-related cataracts. When MBP was purified from eosinophils or recombinant MBP was added to LEC culture medium, cell viability decreased in a concentration-dependent manner, but an MBP antibody neutralized the cytotoxic effect of this protein towards these cells. These results were consistent with the flow of MBP into the aqueous humor from the blood due to a compromised blood–aqueous barrier. Thus, MBP could further penetrate the lens capsule and adhere to LECs, resulting in decreased cell viability and the development of atopic cataracts.",
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Mechanism of atopic cataract caused by eosinophil granule major basic protein. / Yamamoto, Naoki; Hiramatsu, Noriko; Isogai, Sumito; Kondo, Masashi; Imaizumi, Kazuyoshi; Horiguchi, Masayuki.

In: Medical Molecular Morphology, 01.01.2019.

Research output: Contribution to journalArticle

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