Mechanism of cell interactions with water-dispersed carbon nanohorns

Tatsuya Murakami, Masashi Nakatani, Masahiro Kokubo, Hirotaka Nakatsuji, Mami Inada, Hiroshi Imahori, Masako Yudasaka, Sumio Iijima, Kunihiro Tsuchida

Research output: Contribution to journalArticle

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Abstract

The interaction of water-dispersed nanocarbon materials with mammalian cells has been a matter of great interest. Here, we show a possible mechanism of cell interaction with single-walled carbon nanohorns (CNHs). Individually isolated CNHs in H2O, which were noncovalently modified with a polyethylene glycol (PEG)-doxorubicin conjugate, were visualized by atomic force microscopy. Upon treatment of four types of mammalian cells with the PEG-modified CNHs (PEG-d-CNHs), only two cell lines showed a strong affinity to CNHs. The cell interaction with PEG-d-CNHs was temperature-sensitive, suggesting the involvement of endocytosis in it. The CNHs were clearly observed on the ruffled plasma membranes as well as in the membrane compartments inside the cells by electron microscopy. Cell sorter analysis demonstrated that this cell interaction with PEG-d-CNHs was effectively inhibited by treatment with cytochalasin B, an inhibitor of membrane ruffling and macropinocytosis. Under our experimental conditions, no significant cytotoxicity was detected. These data suggest that macropinocytosis after membrane ruffling is responsible for the cell interaction.

Original languageEnglish
Pages (from-to)402-407
Number of pages6
JournalNanoscience and Nanotechnology Letters
Volume5
Issue number3
DOIs
Publication statusPublished - 01-03-2013

Fingerprint

Nanohorns
Carbon
Water
Polyethylene glycols
Cells
Membranes
Cytochalasin B
Cell membranes
Cytotoxicity
Doxorubicin
Electron microscopy
Atomic force microscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Murakami, Tatsuya ; Nakatani, Masashi ; Kokubo, Masahiro ; Nakatsuji, Hirotaka ; Inada, Mami ; Imahori, Hiroshi ; Yudasaka, Masako ; Iijima, Sumio ; Tsuchida, Kunihiro. / Mechanism of cell interactions with water-dispersed carbon nanohorns. In: Nanoscience and Nanotechnology Letters. 2013 ; Vol. 5, No. 3. pp. 402-407.
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Murakami, T, Nakatani, M, Kokubo, M, Nakatsuji, H, Inada, M, Imahori, H, Yudasaka, M, Iijima, S & Tsuchida, K 2013, 'Mechanism of cell interactions with water-dispersed carbon nanohorns', Nanoscience and Nanotechnology Letters, vol. 5, no. 3, pp. 402-407. https://doi.org/10.1166/nnl.2013.1544

Mechanism of cell interactions with water-dispersed carbon nanohorns. / Murakami, Tatsuya; Nakatani, Masashi; Kokubo, Masahiro; Nakatsuji, Hirotaka; Inada, Mami; Imahori, Hiroshi; Yudasaka, Masako; Iijima, Sumio; Tsuchida, Kunihiro.

In: Nanoscience and Nanotechnology Letters, Vol. 5, No. 3, 01.03.2013, p. 402-407.

Research output: Contribution to journalArticle

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