Mechanism of phospholipase D activation induced by prostaglandin D 2 in osteoblast-like cells: Function of Ca 2+ /calmodulin

Yoko Imamura, Osamu Kozawa, Atsushi Suzuki, Yasuko Watanabe, Hidehiko Saito, Yutaka Oiso

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Prostaglandin D 2 (PGD 2 ) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD 2 on the formation of inositol phosphates (EC 50 was 20 nM) was more potent than that on the formation of choline (EC 50 was 0.5 μM). The formation of choline stimulated by a combination of PGD 2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD 2 -induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD 2 stimulated Ca 2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca 2+ by EGTA reduced the PGD 2 -induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD 2 -induced choline formation. These results strongly suggest that PGD 2 activates phospholipase D in a Ca 2+ /calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD 2 -induced activation of phospholipase D.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalCellular Signalling
Volume7
Issue number1
DOIs
Publication statusPublished - 01-01-1995
Externally publishedYes

Fingerprint

Prostaglandins D
Phospholipase D
Calmodulin
Osteoblasts
Choline
Protein Kinase Inhibitors
Protein Kinase C
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Trifluoperazine
Inositol Phosphates
Staurosporine
Egtazic Acid
Extracellular Space
Tetradecanoylphorbol Acetate

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Imamura, Yoko ; Kozawa, Osamu ; Suzuki, Atsushi ; Watanabe, Yasuko ; Saito, Hidehiko ; Oiso, Yutaka. / Mechanism of phospholipase D activation induced by prostaglandin D 2 in osteoblast-like cells : Function of Ca 2+ /calmodulin. In: Cellular Signalling. 1995 ; Vol. 7, No. 1. pp. 45-51.
@article{9b6c656c811948a1b4092cb21294e01a,
title = "Mechanism of phospholipase D activation induced by prostaglandin D 2 in osteoblast-like cells: Function of Ca 2+ /calmodulin",
abstract = "Prostaglandin D 2 (PGD 2 ) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD 2 on the formation of inositol phosphates (EC 50 was 20 nM) was more potent than that on the formation of choline (EC 50 was 0.5 μM). The formation of choline stimulated by a combination of PGD 2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD 2 -induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD 2 stimulated Ca 2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca 2+ by EGTA reduced the PGD 2 -induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD 2 -induced choline formation. These results strongly suggest that PGD 2 activates phospholipase D in a Ca 2+ /calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD 2 -induced activation of phospholipase D.",
author = "Yoko Imamura and Osamu Kozawa and Atsushi Suzuki and Yasuko Watanabe and Hidehiko Saito and Yutaka Oiso",
year = "1995",
month = "1",
day = "1",
doi = "10.1016/0898-6568(94)00059-K",
language = "English",
volume = "7",
pages = "45--51",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier Inc.",
number = "1",

}

Mechanism of phospholipase D activation induced by prostaglandin D 2 in osteoblast-like cells : Function of Ca 2+ /calmodulin. / Imamura, Yoko; Kozawa, Osamu; Suzuki, Atsushi; Watanabe, Yasuko; Saito, Hidehiko; Oiso, Yutaka.

In: Cellular Signalling, Vol. 7, No. 1, 01.01.1995, p. 45-51.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mechanism of phospholipase D activation induced by prostaglandin D 2 in osteoblast-like cells

T2 - Function of Ca 2+ /calmodulin

AU - Imamura, Yoko

AU - Kozawa, Osamu

AU - Suzuki, Atsushi

AU - Watanabe, Yasuko

AU - Saito, Hidehiko

AU - Oiso, Yutaka

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Prostaglandin D 2 (PGD 2 ) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD 2 on the formation of inositol phosphates (EC 50 was 20 nM) was more potent than that on the formation of choline (EC 50 was 0.5 μM). The formation of choline stimulated by a combination of PGD 2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD 2 -induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD 2 stimulated Ca 2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca 2+ by EGTA reduced the PGD 2 -induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD 2 -induced choline formation. These results strongly suggest that PGD 2 activates phospholipase D in a Ca 2+ /calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD 2 -induced activation of phospholipase D.

AB - Prostaglandin D 2 (PGD 2 ) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD 2 on the formation of inositol phosphates (EC 50 was 20 nM) was more potent than that on the formation of choline (EC 50 was 0.5 μM). The formation of choline stimulated by a combination of PGD 2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD 2 -induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD 2 stimulated Ca 2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca 2+ by EGTA reduced the PGD 2 -induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD 2 -induced choline formation. These results strongly suggest that PGD 2 activates phospholipase D in a Ca 2+ /calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD 2 -induced activation of phospholipase D.

UR - http://www.scopus.com/inward/record.url?scp=0028904485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028904485&partnerID=8YFLogxK

U2 - 10.1016/0898-6568(94)00059-K

DO - 10.1016/0898-6568(94)00059-K

M3 - Article

C2 - 7756110

AN - SCOPUS:0028904485

VL - 7

SP - 45

EP - 51

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 1

ER -