Mechanism of phospholipase D activation induced by prostaglandin D2 in osteoblast-like cells: Function of Ca2+/calmodulin

Yoko Imamura, Osamu Kozawa, Atsushi Suzuki, Yasuko Watanabe, Hidehiko Saito, Yutaka Oiso

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Prostaglandin D2 (PGD2) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD2 on the formation of inositol phosphates (EC50 was 20 nM) was more potent than that on the formation of choline (EC50 was 0.5 μM). The formation of choline stimulated by a combination of PGD2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD2-induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD2 stimulated Ca2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca2+ by EGTA reduced the PGD2-induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD2-induced choline formation. These results strongly suggest that PGD2 activates phospholipase D in a Ca2+/calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD2-induced activation of phospholipase D.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalCellular Signalling
Volume7
Issue number1
DOIs
Publication statusPublished - 01-1995

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Prostaglandin D2
Phospholipase D
Calmodulin
Osteoblasts
Choline
Protein Kinase Inhibitors
Protein Kinase C
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Trifluoperazine
Inositol Phosphates
Staurosporine
Egtazic Acid
Extracellular Space
Tetradecanoylphorbol Acetate

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Imamura, Yoko ; Kozawa, Osamu ; Suzuki, Atsushi ; Watanabe, Yasuko ; Saito, Hidehiko ; Oiso, Yutaka. / Mechanism of phospholipase D activation induced by prostaglandin D2 in osteoblast-like cells : Function of Ca2+/calmodulin. In: Cellular Signalling. 1995 ; Vol. 7, No. 1. pp. 45-51.
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Mechanism of phospholipase D activation induced by prostaglandin D2 in osteoblast-like cells : Function of Ca2+/calmodulin. / Imamura, Yoko; Kozawa, Osamu; Suzuki, Atsushi; Watanabe, Yasuko; Saito, Hidehiko; Oiso, Yutaka.

In: Cellular Signalling, Vol. 7, No. 1, 01.1995, p. 45-51.

Research output: Contribution to journalArticle

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AB - Prostaglandin D2 (PGD2) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 μM. The effect of PGD2 on the formation of inositol phosphates (EC50 was 20 nM) was more potent than that on the formation of choline (EC50 was 0.5 μM). The formation of choline stimulated by a combination of PGD2 and 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, was additive. Staurosporine, an inhibitor for protein kinases, enhanced the PGD2-induced formation of choline, but H-7, another inhibitor for protein kinases, had little effect. PGD2 stimulated Ca2+ influx from extracellular space dose-dependently. The depletion of extracellular Ca2+ by EGTA reduced the PGD2-induced formation of choline. W-7 and trifluoperazine dihydrochloride, antagonists of calmodulin, dose-dependently inhibited the PGD2-induced choline formation. These results strongly suggest that PGD2 activates phospholipase D in a Ca2+/calmodulin dependent manner in osteoblast-like cells, and that protein kinase C is not essential for the PGD2-induced activation of phospholipase D.

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