Abstract
1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1800-1804 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 581 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 01-05-2007 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes. / Kikuchi, Ryosuke; Sobue, Sayaka; Murakami, Masashi; Ito, Hiromi; Kimura, Ami; Iwasaki, Takashi; Shibayama, Shuji; Takagi, Akira; Kojima, Tetsuhito; Suzuki, Motoshi; Banno, Yoshiko; Nozawa, Yoshinori; Murate, Takashi.
In: FEBS Letters, Vol. 581, No. 9, 01.05.2007, p. 1800-1804.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes
AU - Kikuchi, Ryosuke
AU - Sobue, Sayaka
AU - Murakami, Masashi
AU - Ito, Hiromi
AU - Kimura, Ami
AU - Iwasaki, Takashi
AU - Shibayama, Shuji
AU - Takagi, Akira
AU - Kojima, Tetsuhito
AU - Suzuki, Motoshi
AU - Banno, Yoshiko
AU - Nozawa, Yoshinori
AU - Murate, Takashi
PY - 2007/5/1
Y1 - 2007/5/1
N2 - 1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.
AB - 1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.
UR - http://www.scopus.com/inward/record.url?scp=34247162737&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247162737&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2007.03.073
DO - 10.1016/j.febslet.2007.03.073
M3 - Article
C2 - 17433303
AN - SCOPUS:34247162737
VL - 581
SP - 1800
EP - 1804
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 9
ER -