Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes

Ryosuke Kikuchi; Sayaka Sobue; Masashi Murakami; Hiromi Ito; Ami Kimura; Takashi Iwasaki; Shuji Shibayama; Akira Takagi; Tetsuhito Kojima; Motoshi Suzuki; Yoshiko Banno; Yoshinori Nozawa; Takashi Murate

doi:10.1016/j.febslet.2007.03.073

Mechanism of vitamin D₃-induced transcription of phospholipase D1 in HaCat human keratinocytes

Ryosuke Kikuchi, Sayaka Sobue, Masashi Murakami, Hiromi Ito, Ami Kimura, Takashi Iwasaki, Shuji Shibayama, Akira Takagi, Tetsuhito Kojima, Motoshi Suzuki, Yoshiko Banno, Yoshinori Nozawa, Takashi Murate

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

1α,25-Dihydroxyvitamin D₃ (VitD₃) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD₃ increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD₃ receptor (VDR) into HEK293 cells, which are originally VitD₃-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD₃, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.

Original language	English
Pages (from-to)	1800-1804
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2007.03.073
Publication status	Published - 01-05-2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.03.073

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title = "Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes",

abstract = "1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.",

keywords = "1α,25-Dihydroxyvitamin D, PLD1 gene expression, Promoter analysis, RXRα, VDR",

author = "Ryosuke Kikuchi and Sayaka Sobue and Masashi Murakami and Hiromi Ito and Ami Kimura and Takashi Iwasaki and Shuji Shibayama and Akira Takagi and Tetsuhito Kojima and Motoshi Suzuki and Yoshiko Banno and Yoshinori Nozawa and Takashi Murate",

year = "2007",

month = may,

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doi = "10.1016/j.febslet.2007.03.073",

language = "English",

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TY - JOUR

T1 - Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes

AU - Kikuchi, Ryosuke

AU - Sobue, Sayaka

AU - Murakami, Masashi

AU - Ito, Hiromi

AU - Kimura, Ami

AU - Iwasaki, Takashi

AU - Shibayama, Shuji

AU - Takagi, Akira

AU - Kojima, Tetsuhito

AU - Suzuki, Motoshi

AU - Banno, Yoshiko

AU - Nozawa, Yoshinori

AU - Murate, Takashi

PY - 2007/5/1

Y1 - 2007/5/1

N2 - 1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.

AB - 1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.

KW - 1α,25-Dihydroxyvitamin D

KW - PLD1 gene expression

KW - Promoter analysis

KW - RXRα

KW - VDR

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JO - FEBS Letters

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Mechanism of vitamin D₃-induced transcription of phospholipase D1 in HaCat human keratinocytes

Abstract

All Science Journal Classification (ASJC) codes

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