TY - JOUR
T1 - Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes
AU - Kikuchi, Ryosuke
AU - Sobue, Sayaka
AU - Murakami, Masashi
AU - Ito, Hiromi
AU - Kimura, Ami
AU - Iwasaki, Takashi
AU - Shibayama, Shuji
AU - Takagi, Akira
AU - Kojima, Tetsuhito
AU - Suzuki, Motoshi
AU - Banno, Yoshiko
AU - Nozawa, Yoshinori
AU - Murate, Takashi
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - 1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.
AB - 1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.
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U2 - 10.1016/j.febslet.2007.03.073
DO - 10.1016/j.febslet.2007.03.073
M3 - Article
C2 - 17433303
AN - SCOPUS:34247162737
VL - 581
SP - 1800
EP - 1804
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 9
ER -