Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes

Ryosuke Kikuchi, Sayaka Sobue, Masashi Murakami, Hiromi Ito, Ami Kimura, Takashi Iwasaki, Shuji Shibayama, Akira Takagi, Tetsuhito Kojima, Motoshi Suzuki, Yoshiko Banno, Yoshinori Nozawa, Takashi Murate

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.

Original languageEnglish
Pages (from-to)1800-1804
Number of pages5
JournalFEBS Letters
Volume581
Issue number9
DOIs
Publication statusPublished - 01-05-2007

Fingerprint

Cholecalciferol
Transcription
Keratinocytes
Calcitriol
Gene expression
Calcitriol Receptors
Gene Expression
Vitamin D
Assays
Retinoid X Receptors
Chromatin Immunoprecipitation
HEK293 Cells
Electrophoretic Mobility Shift Assay
Electrophoresis
Chromatin
Exons
phospholipase D1
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kikuchi, R., Sobue, S., Murakami, M., Ito, H., Kimura, A., Iwasaki, T., ... Murate, T. (2007). Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes. FEBS Letters, 581(9), 1800-1804. https://doi.org/10.1016/j.febslet.2007.03.073
Kikuchi, Ryosuke ; Sobue, Sayaka ; Murakami, Masashi ; Ito, Hiromi ; Kimura, Ami ; Iwasaki, Takashi ; Shibayama, Shuji ; Takagi, Akira ; Kojima, Tetsuhito ; Suzuki, Motoshi ; Banno, Yoshiko ; Nozawa, Yoshinori ; Murate, Takashi. / Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes. In: FEBS Letters. 2007 ; Vol. 581, No. 9. pp. 1800-1804.
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abstract = "1α,25-Dihydroxyvitamin D3 (VitD3) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD3-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.",
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Kikuchi, R, Sobue, S, Murakami, M, Ito, H, Kimura, A, Iwasaki, T, Shibayama, S, Takagi, A, Kojima, T, Suzuki, M, Banno, Y, Nozawa, Y & Murate, T 2007, 'Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes', FEBS Letters, vol. 581, no. 9, pp. 1800-1804. https://doi.org/10.1016/j.febslet.2007.03.073

Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes. / Kikuchi, Ryosuke; Sobue, Sayaka; Murakami, Masashi; Ito, Hiromi; Kimura, Ami; Iwasaki, Takashi; Shibayama, Shuji; Takagi, Akira; Kojima, Tetsuhito; Suzuki, Motoshi; Banno, Yoshiko; Nozawa, Yoshinori; Murate, Takashi.

In: FEBS Letters, Vol. 581, No. 9, 01.05.2007, p. 1800-1804.

Research output: Contribution to journalArticle

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T1 - Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes

AU - Kikuchi, Ryosuke

AU - Sobue, Sayaka

AU - Murakami, Masashi

AU - Ito, Hiromi

AU - Kimura, Ami

AU - Iwasaki, Takashi

AU - Shibayama, Shuji

AU - Takagi, Akira

AU - Kojima, Tetsuhito

AU - Suzuki, Motoshi

AU - Banno, Yoshiko

AU - Nozawa, Yoshinori

AU - Murate, Takashi

PY - 2007/5/1

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