Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.

S. Izuta, M. Saneyoshi, T. Sakurai, Motoshi Suzuki, K. Kojima, S. Yoshida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.

Original languageEnglish
Pages (from-to)79-80
Number of pages2
JournalNucleic acids symposium series
Issue number25
Publication statusPublished - 01-01-1991

Fingerprint

Nucleotides
splenotritin
HIV
DNA Primers
Single-Stranded DNA
DNA
Thymidine
Testis
DNA polymerase gamma
3'-azido-3'-deoxythymidine 5'-triphosphate
2',3'-dideoxythymidine triphosphate
thymidine 5'-triphosphate

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Izuta, S. ; Saneyoshi, M. ; Sakurai, T. ; Suzuki, Motoshi ; Kojima, K. ; Yoshida, S. / Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities. In: Nucleic acids symposium series. 1991 ; No. 25. pp. 79-80.
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Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities. / Izuta, S.; Saneyoshi, M.; Sakurai, T.; Suzuki, Motoshi; Kojima, K.; Yoshida, S.

In: Nucleic acids symposium series, No. 25, 01.01.1991, p. 79-80.

Research output: Contribution to journalArticle

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AU - Yoshida, S.

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