Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.

S. Izuta; M. Saneyoshi; T. Sakurai; M. Suzuki; K. Kojima; S. Yoshida

Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.

S. Izuta, M. Saneyoshi, T. Sakurai, M. Suzuki, K. Kojima, S. Yoshida

Research output: Contribution to journal › Article › peer-review

Abstract

Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.

Original language	English
Pages (from-to)	79-80
Number of pages	2
Journal	Nucleic acids symposium series
Issue number	25
Publication status	Published - 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.",

abstract = "Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.",

author = "S. Izuta and M. Saneyoshi and T. Sakurai and M. Suzuki and K. Kojima and S. Yoshida",

year = "1991",

language = "English",

pages = "79--80",

journal = "Nucleic acids symposium series",

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publisher = "Information Retrieval",

number = "25",

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TY - JOUR

T1 - Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.

AU - Izuta, S.

AU - Saneyoshi, M.

AU - Sakurai, T.

AU - Suzuki, M.

AU - Kojima, K.

AU - Yoshida, S.

PY - 1991

Y1 - 1991

N2 - Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.

AB - Inhibition mechanisms of 5'-triphosphates of 3'-azido-3'-deoxythymidine (AZT-TP) and 3'-deoxythymidine (ddTTP) on extensively purified DNA polymerase gamma from bovine testes were examined by analysis of the products synthesized on singly primed M13mp18 single-stranded DNA or synthetic oligonucleotide template-primer in the presence of analogues. The results indicate that AZT-TP inhibits DNA polymerase gamma in competition with dTTP but is not incorporated into DNA, whereas ddTTP is incorporated into DNA and causes chain termination.

UR - https://www.scopus.com/pages/publications/0026380284

UR - https://www.scopus.com/inward/citedby.url?scp=0026380284&partnerID=8YFLogxK

M3 - Article

C2 - 1842105

AN - SCOPUS:0026380284

SN - 0261-3166

SP - 79

EP - 80

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 25

ER -

Mechanisms of inhibitions of DNA polymerase gamma by nucleotide analogues having anti-HIV activities.

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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