Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface

Takaaki Yamada, Hirohiko Akamatsu, Seiji Hasegawa, Yu Inoue, Yasushi Date, Hiroshi Mizutani, Naoki Yamamoto, Kayoko Matsunaga, Satoru Nakata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It has been reported that melanocytes play important roles in skin and hair pigmentation and are differentiated from melanocyte stem cells (MSCs) residing in the bulge area of hair follicles. Recently, interest has been growing in MSCs because regulation of the upstream of differentiated melanocytes is essential for the determination of skin and hair pigmentation; however, their precise characteristics remain to be elucidated. The aim of this study is to explore cell-surface markers expressed on MSCs in order to understand their characteristics. To explore genes specifically expressed in the bulge region, we classified a hair follicle into four areas, hair bulb, hair bulb to bulge (lower bulge), bulge, and epidermis to bulge (upper bulge), and collected these areas from back skin sections of C57BL/6 mice by laser microdissection. Real-time RT-PCR performed on these areas revealed that Frizzled (Fzd)-4, Fzd7, low density lipoprotein receptor-related protein 5 (Lrp5), and Lrp6, receptors for Wnt molecules, were expressed higher in the bulge area than other areas. Furthermore, FACS analysis showed that populations of Fzd4+ cells and Fzd7+ cells were different from those of Kit+ cells (precursor of melanocytes: melanoblasts). Fzd4+ and Fzd7+ cells isolated by FACS required a longer culture period to differentiate into mature melanocytes than Kit+ cells. Up-regulation of mRNA expressions of melanocyte markers (dopa chrometautomerase: Dct, tyrosinase: Tyr, tyrosinase-related protein 1: Tyrp1) was observed in Fzd4+ and Fzd7+ cells following Kit+ cells during differentiation. These results suggested that Fzd4+ and Fzd7+ cells were more immature than melanoblasts, therefore raising the possibility that Fzd4+ and Fzd7+ cells are MSCs.

Original languageEnglish
Pages (from-to)837-842
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume396
Issue number4
DOIs
Publication statusPublished - 11-06-2010

Fingerprint

Wnt Receptors
Wnt Signaling Pathway
Melanocytes
Stem cells
Stem Cells
Skin
Low Density Lipoprotein Receptor-Related Protein-5
Hair
Microdissection
Dihydroxyphenylalanine
Skin Pigmentation
Monophenol Monooxygenase
Hair Follicle
Genes
Messenger RNA
Molecules
Lasers
Inbred C57BL Mouse
Epidermis
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yamada, Takaaki ; Akamatsu, Hirohiko ; Hasegawa, Seiji ; Inoue, Yu ; Date, Yasushi ; Mizutani, Hiroshi ; Yamamoto, Naoki ; Matsunaga, Kayoko ; Nakata, Satoru. / Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 396, No. 4. pp. 837-842.
@article{3c0e5f0868234b92836807471f3f56b4,
title = "Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface",
abstract = "It has been reported that melanocytes play important roles in skin and hair pigmentation and are differentiated from melanocyte stem cells (MSCs) residing in the bulge area of hair follicles. Recently, interest has been growing in MSCs because regulation of the upstream of differentiated melanocytes is essential for the determination of skin and hair pigmentation; however, their precise characteristics remain to be elucidated. The aim of this study is to explore cell-surface markers expressed on MSCs in order to understand their characteristics. To explore genes specifically expressed in the bulge region, we classified a hair follicle into four areas, hair bulb, hair bulb to bulge (lower bulge), bulge, and epidermis to bulge (upper bulge), and collected these areas from back skin sections of C57BL/6 mice by laser microdissection. Real-time RT-PCR performed on these areas revealed that Frizzled (Fzd)-4, Fzd7, low density lipoprotein receptor-related protein 5 (Lrp5), and Lrp6, receptors for Wnt molecules, were expressed higher in the bulge area than other areas. Furthermore, FACS analysis showed that populations of Fzd4+ cells and Fzd7+ cells were different from those of Kit+ cells (precursor of melanocytes: melanoblasts). Fzd4+ and Fzd7+ cells isolated by FACS required a longer culture period to differentiate into mature melanocytes than Kit+ cells. Up-regulation of mRNA expressions of melanocyte markers (dopa chrometautomerase: Dct, tyrosinase: Tyr, tyrosinase-related protein 1: Tyrp1) was observed in Fzd4+ and Fzd7+ cells following Kit+ cells during differentiation. These results suggested that Fzd4+ and Fzd7+ cells were more immature than melanoblasts, therefore raising the possibility that Fzd4+ and Fzd7+ cells are MSCs.",
author = "Takaaki Yamada and Hirohiko Akamatsu and Seiji Hasegawa and Yu Inoue and Yasushi Date and Hiroshi Mizutani and Naoki Yamamoto and Kayoko Matsunaga and Satoru Nakata",
year = "2010",
month = "6",
day = "11",
doi = "10.1016/j.bbrc.2010.04.167",
language = "English",
volume = "396",
pages = "837--842",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

Yamada, T, Akamatsu, H, Hasegawa, S, Inoue, Y, Date, Y, Mizutani, H, Yamamoto, N, Matsunaga, K & Nakata, S 2010, 'Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface', Biochemical and Biophysical Research Communications, vol. 396, no. 4, pp. 837-842. https://doi.org/10.1016/j.bbrc.2010.04.167

Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface. / Yamada, Takaaki; Akamatsu, Hirohiko; Hasegawa, Seiji; Inoue, Yu; Date, Yasushi; Mizutani, Hiroshi; Yamamoto, Naoki; Matsunaga, Kayoko; Nakata, Satoru.

In: Biochemical and Biophysical Research Communications, Vol. 396, No. 4, 11.06.2010, p. 837-842.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface

AU - Yamada, Takaaki

AU - Akamatsu, Hirohiko

AU - Hasegawa, Seiji

AU - Inoue, Yu

AU - Date, Yasushi

AU - Mizutani, Hiroshi

AU - Yamamoto, Naoki

AU - Matsunaga, Kayoko

AU - Nakata, Satoru

PY - 2010/6/11

Y1 - 2010/6/11

N2 - It has been reported that melanocytes play important roles in skin and hair pigmentation and are differentiated from melanocyte stem cells (MSCs) residing in the bulge area of hair follicles. Recently, interest has been growing in MSCs because regulation of the upstream of differentiated melanocytes is essential for the determination of skin and hair pigmentation; however, their precise characteristics remain to be elucidated. The aim of this study is to explore cell-surface markers expressed on MSCs in order to understand their characteristics. To explore genes specifically expressed in the bulge region, we classified a hair follicle into four areas, hair bulb, hair bulb to bulge (lower bulge), bulge, and epidermis to bulge (upper bulge), and collected these areas from back skin sections of C57BL/6 mice by laser microdissection. Real-time RT-PCR performed on these areas revealed that Frizzled (Fzd)-4, Fzd7, low density lipoprotein receptor-related protein 5 (Lrp5), and Lrp6, receptors for Wnt molecules, were expressed higher in the bulge area than other areas. Furthermore, FACS analysis showed that populations of Fzd4+ cells and Fzd7+ cells were different from those of Kit+ cells (precursor of melanocytes: melanoblasts). Fzd4+ and Fzd7+ cells isolated by FACS required a longer culture period to differentiate into mature melanocytes than Kit+ cells. Up-regulation of mRNA expressions of melanocyte markers (dopa chrometautomerase: Dct, tyrosinase: Tyr, tyrosinase-related protein 1: Tyrp1) was observed in Fzd4+ and Fzd7+ cells following Kit+ cells during differentiation. These results suggested that Fzd4+ and Fzd7+ cells were more immature than melanoblasts, therefore raising the possibility that Fzd4+ and Fzd7+ cells are MSCs.

AB - It has been reported that melanocytes play important roles in skin and hair pigmentation and are differentiated from melanocyte stem cells (MSCs) residing in the bulge area of hair follicles. Recently, interest has been growing in MSCs because regulation of the upstream of differentiated melanocytes is essential for the determination of skin and hair pigmentation; however, their precise characteristics remain to be elucidated. The aim of this study is to explore cell-surface markers expressed on MSCs in order to understand their characteristics. To explore genes specifically expressed in the bulge region, we classified a hair follicle into four areas, hair bulb, hair bulb to bulge (lower bulge), bulge, and epidermis to bulge (upper bulge), and collected these areas from back skin sections of C57BL/6 mice by laser microdissection. Real-time RT-PCR performed on these areas revealed that Frizzled (Fzd)-4, Fzd7, low density lipoprotein receptor-related protein 5 (Lrp5), and Lrp6, receptors for Wnt molecules, were expressed higher in the bulge area than other areas. Furthermore, FACS analysis showed that populations of Fzd4+ cells and Fzd7+ cells were different from those of Kit+ cells (precursor of melanocytes: melanoblasts). Fzd4+ and Fzd7+ cells isolated by FACS required a longer culture period to differentiate into mature melanocytes than Kit+ cells. Up-regulation of mRNA expressions of melanocyte markers (dopa chrometautomerase: Dct, tyrosinase: Tyr, tyrosinase-related protein 1: Tyrp1) was observed in Fzd4+ and Fzd7+ cells following Kit+ cells during differentiation. These results suggested that Fzd4+ and Fzd7+ cells were more immature than melanoblasts, therefore raising the possibility that Fzd4+ and Fzd7+ cells are MSCs.

UR - http://www.scopus.com/inward/record.url?scp=77953289485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953289485&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2010.04.167

DO - 10.1016/j.bbrc.2010.04.167

M3 - Article

C2 - 20450888

AN - SCOPUS:77953289485

VL - 396

SP - 837

EP - 842

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -