Mesenchymal stem cells attenuate ischemia–reperfusion injury after prolonged cold ischemia in a mouse model of lung transplantation: a preliminary study

Tatsuaki Watanabe, Yasushi Hoshikawa, Naoya Ishibashi, Hirotoshi Suzuki, Hirotsugu Notsuda, Yui Watanabe, Masafumi Noda, Masahiko Kanehira, Shinya Ohkouchi, Takashi Kondo, Yoshinori Okada

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Abstract

Purpose: Mesenchymal stem cells (MSCs) suppress inflammation and immune responses. We conducted this study to find out if MSCs attenuate ischemia–reperfusion injury in a mouse model of lung transplantation. Methods: C57BL/6J mouse lungs perfused with low-potassium dextran glucose solution were preserved at 4 °C for 18 h. Human MSCs were slowly injected into the left pulmonary artery of the lung grafts, and orthotopic left lung transplantation was then performed. The lung isografts were reperfused for 6 h, and bronchoalveolar lavage fluid (BALF) from the left lung graft was collected. We measured the protein concentration, cell count, and proinflammatory cytokine concentrations in the BALF. Results: The protein concentration and cell count in the BALF were significantly lower in the MSC-administered grafts than in the PBS-administered controls. Concentrations of proinflammatory cytokines, including IL-1β, IL-17A, and TNF-α, in BALF tended to be lower in the MSC-administered grafts than in the controls, but the difference was not significant. Conclusion: The pre-transplant administration of MSCs via the pulmonary artery of the lung graft attenuated ischemia–reperfusion injury after prolonged cold ischemia in this mouse model of lung transplantation.

Original languageEnglish
Pages (from-to)425-431
Number of pages7
JournalSurgery Today
Volume47
Issue number4
DOIs
Publication statusPublished - 01-04-2017
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Watanabe, T., Hoshikawa, Y., Ishibashi, N., Suzuki, H., Notsuda, H., Watanabe, Y., Noda, M., Kanehira, M., Ohkouchi, S., Kondo, T., & Okada, Y. (2017). Mesenchymal stem cells attenuate ischemia–reperfusion injury after prolonged cold ischemia in a mouse model of lung transplantation: a preliminary study. Surgery Today, 47(4), 425-431. https://doi.org/10.1007/s00595-016-1391-8