Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+

Takahide Nomura, Masatsugu Ohtsuki, Toshiko Watanabe, Seiko Hasegawa, Shigeru Matsui, Akira Tomita, Chiho Ichinose, Hiroko Nomura, Yasumichi Hagino

Research output: Contribution to journalArticle

Abstract

We examined the metabolic effects of glibenclamide, a potent second-generation sulfonylurea, in isolated rat hepatocytes incubated in the absence of extracellular Ca2+. We first demonstrated in the present study that glibenclamide caused a significant increase in basal glucose release and lactate production without any modification of intracellular Ca2+ concentration or cAMP levels in isolated rat hepatocytes. Furthermore, glibenclamide inhibited the noradrenaline-induced increase in cAMP accumulation, while activation of glycogenolysis by noradrenaline was not suppressed by this agent. Our data indicate that glibenclamide exerts its metabolic effects independent of intracellular Ca2+ mobilization and cAMP accumulation.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalThe Japanese Journal of Pharmacology
Volume69
Issue number2
DOIs
Publication statusPublished - 01-01-1995

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Glyburide
Hepatocytes
Norepinephrine
Glycogenolysis
Lactic Acid
Glucose

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Nomura, T., Ohtsuki, M., Watanabe, T., Hasegawa, S., Matsui, S., Tomita, A., ... Hagino, Y. (1995). Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+. The Japanese Journal of Pharmacology, 69(2), 173-176. https://doi.org/10.1254/jjp.69.173
Nomura, Takahide ; Ohtsuki, Masatsugu ; Watanabe, Toshiko ; Hasegawa, Seiko ; Matsui, Shigeru ; Tomita, Akira ; Ichinose, Chiho ; Nomura, Hiroko ; Hagino, Yasumichi. / Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+. In: The Japanese Journal of Pharmacology. 1995 ; Vol. 69, No. 2. pp. 173-176.
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Nomura, T, Ohtsuki, M, Watanabe, T, Hasegawa, S, Matsui, S, Tomita, A, Ichinose, C, Nomura, H & Hagino, Y 1995, 'Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+', The Japanese Journal of Pharmacology, vol. 69, no. 2, pp. 173-176. https://doi.org/10.1254/jjp.69.173

Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+. / Nomura, Takahide; Ohtsuki, Masatsugu; Watanabe, Toshiko; Hasegawa, Seiko; Matsui, Shigeru; Tomita, Akira; Ichinose, Chiho; Nomura, Hiroko; Hagino, Yasumichi.

In: The Japanese Journal of Pharmacology, Vol. 69, No. 2, 01.01.1995, p. 173-176.

Research output: Contribution to journalArticle

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AU - Nomura, Takahide

AU - Ohtsuki, Masatsugu

AU - Watanabe, Toshiko

AU - Hasegawa, Seiko

AU - Matsui, Shigeru

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AU - Ichinose, Chiho

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