Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+

Takahide Nomura, Masatsugu Ohtsuki, Toshiko Watanabe, Seiko Hasegawa, Shigeru Matsui, Akira Tomita, Chiho Sumi-Ichinose, Hiroko Nomura, Yasumichi Hagino

Research output: Contribution to journalArticle

Abstract

We examined the metabolic effects of glibenclamide, a potent second-generation sulfonylurea, in isolated rat hepatocytes incubated in the absence of extracellular Ca2+. We first demonstrated in the present study that glibenclamide caused a significant increase in basal glucose release and lactate production without any modification of intracellular Ca2+ concentration or cAMP levels in isolated rat hepatocytes. Furthermore, glibenclamide inhibited the noradrenaline-induced increase in cAMP accumulation, while activation of glycogenolysis by noradrenaline was not suppressed by this agent. Our data indicate that glibenclamide exerts its metabolic effects independent of intracellular Ca2+ mobilization and cAMP accumulation.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalThe Japanese Journal of Pharmacology
Volume69
Issue number2
DOIs
Publication statusPublished - 01-01-1995

All Science Journal Classification (ASJC) codes

  • Pharmacology

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    Nomura, T., Ohtsuki, M., Watanabe, T., Hasegawa, S., Matsui, S., Tomita, A., Sumi-Ichinose, C., Nomura, H., & Hagino, Y. (1995). Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+. The Japanese Journal of Pharmacology, 69(2), 173-176. https://doi.org/10.1254/jjp.69.173