Mice deficient in protein tyrosine phosphatase receptor type Z (PTPRZ) show reduced responsivity to methamphetamine despite an enhanced response to novelty

Akihiro Fujikawa, Yukihiro Noda, Hideko Yamamoto, Naomi Tanga, Gaku Sakaguchi, Satoko Hattori, Wen Jie Song, Ichiro Sora, Toshitaka Nabeshima, Goro Katsuura, Masaharu Noda

Research output: Contribution to journalArticle

Abstract

Methamphetamine (METH), a commonly abused drug, elevates extracellular dopamine (DA) levels by inducing DA efflux through the DA transporter (DAT). Emerging evidence in rodent models suggests that locomotor responses to a novel inescapable open field may predict behavioral responses to abused drugs; METH produces more potent stimulant effects in high responders to novelty than in low responders. We herein found that mice deficient in protein tyrosine phosphatase receptor type Z (Ptprz-KO) exhibited an enhanced behavioral response to novelty; however, METH-induced hyperlocomotion was significantly lower in Ptprz-KO than in wild-type mice when METH was administered at a non-toxic dose of 1 mg per kg body weight (bdw). Single-cell RT-PCR revealed that the majority of midbrain DA neurons expressed PTPRZ. No histological alterations were observed in the mesolimbic or nigrostriatal dopaminergic pathways in Ptprz-KO brains; however, a significant decrease was noted in brain DA turnover, suggesting functional alterations. In vivo microdialysis experiments revealed that METH-evoked DA release in the nucleus accumbens was significantly lower in Ptprz-KO mice than in wild-type mice. Consistent with this result, Ptprz-KO mice showed significantly fewer cell surface DAT as well as weaker DA uptake activity in striatal synaptosomes prepared 1 hr after the administration of METH than wild-type mice, while no significant differences were observed in the two groups treated with saline. These results indicate that the high response phenotype of Ptprz-KO mice to novelty may not be simply attributed to hyper-dopaminergic activity, and that deficits in PTPRZ reduce the effects of METH by reducing DAT activity.

Original languageEnglish
Article numbere0221205
JournalPloS one
Volume14
Issue number8
DOIs
Publication statusPublished - 01-01-2019

Fingerprint

Class 5 Receptor-Like Protein Tyrosine Phosphatases
protein-tyrosine-phosphatase
Methamphetamine
dopamine
Dopamine
receptors
mice
transporters
brain
Brain
synaptosomes
Corpus Striatum
drugs
Dopamine Plasma Membrane Transport Proteins
Synaptosomes
Dopaminergic Neurons
Microdialysis
Nucleus Accumbens
Mesencephalon
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Fujikawa, Akihiro ; Noda, Yukihiro ; Yamamoto, Hideko ; Tanga, Naomi ; Sakaguchi, Gaku ; Hattori, Satoko ; Song, Wen Jie ; Sora, Ichiro ; Nabeshima, Toshitaka ; Katsuura, Goro ; Noda, Masaharu. / Mice deficient in protein tyrosine phosphatase receptor type Z (PTPRZ) show reduced responsivity to methamphetamine despite an enhanced response to novelty. In: PloS one. 2019 ; Vol. 14, No. 8.
@article{650540a8d47e4ba485b121773e4eef35,
title = "Mice deficient in protein tyrosine phosphatase receptor type Z (PTPRZ) show reduced responsivity to methamphetamine despite an enhanced response to novelty",
abstract = "Methamphetamine (METH), a commonly abused drug, elevates extracellular dopamine (DA) levels by inducing DA efflux through the DA transporter (DAT). Emerging evidence in rodent models suggests that locomotor responses to a novel inescapable open field may predict behavioral responses to abused drugs; METH produces more potent stimulant effects in high responders to novelty than in low responders. We herein found that mice deficient in protein tyrosine phosphatase receptor type Z (Ptprz-KO) exhibited an enhanced behavioral response to novelty; however, METH-induced hyperlocomotion was significantly lower in Ptprz-KO than in wild-type mice when METH was administered at a non-toxic dose of 1 mg per kg body weight (bdw). Single-cell RT-PCR revealed that the majority of midbrain DA neurons expressed PTPRZ. No histological alterations were observed in the mesolimbic or nigrostriatal dopaminergic pathways in Ptprz-KO brains; however, a significant decrease was noted in brain DA turnover, suggesting functional alterations. In vivo microdialysis experiments revealed that METH-evoked DA release in the nucleus accumbens was significantly lower in Ptprz-KO mice than in wild-type mice. Consistent with this result, Ptprz-KO mice showed significantly fewer cell surface DAT as well as weaker DA uptake activity in striatal synaptosomes prepared 1 hr after the administration of METH than wild-type mice, while no significant differences were observed in the two groups treated with saline. These results indicate that the high response phenotype of Ptprz-KO mice to novelty may not be simply attributed to hyper-dopaminergic activity, and that deficits in PTPRZ reduce the effects of METH by reducing DAT activity.",
author = "Akihiro Fujikawa and Yukihiro Noda and Hideko Yamamoto and Naomi Tanga and Gaku Sakaguchi and Satoko Hattori and Song, {Wen Jie} and Ichiro Sora and Toshitaka Nabeshima and Goro Katsuura and Masaharu Noda",
year = "2019",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0221205",
language = "English",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

Mice deficient in protein tyrosine phosphatase receptor type Z (PTPRZ) show reduced responsivity to methamphetamine despite an enhanced response to novelty. / Fujikawa, Akihiro; Noda, Yukihiro; Yamamoto, Hideko; Tanga, Naomi; Sakaguchi, Gaku; Hattori, Satoko; Song, Wen Jie; Sora, Ichiro; Nabeshima, Toshitaka; Katsuura, Goro; Noda, Masaharu.

In: PloS one, Vol. 14, No. 8, e0221205, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mice deficient in protein tyrosine phosphatase receptor type Z (PTPRZ) show reduced responsivity to methamphetamine despite an enhanced response to novelty

AU - Fujikawa, Akihiro

AU - Noda, Yukihiro

AU - Yamamoto, Hideko

AU - Tanga, Naomi

AU - Sakaguchi, Gaku

AU - Hattori, Satoko

AU - Song, Wen Jie

AU - Sora, Ichiro

AU - Nabeshima, Toshitaka

AU - Katsuura, Goro

AU - Noda, Masaharu

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Methamphetamine (METH), a commonly abused drug, elevates extracellular dopamine (DA) levels by inducing DA efflux through the DA transporter (DAT). Emerging evidence in rodent models suggests that locomotor responses to a novel inescapable open field may predict behavioral responses to abused drugs; METH produces more potent stimulant effects in high responders to novelty than in low responders. We herein found that mice deficient in protein tyrosine phosphatase receptor type Z (Ptprz-KO) exhibited an enhanced behavioral response to novelty; however, METH-induced hyperlocomotion was significantly lower in Ptprz-KO than in wild-type mice when METH was administered at a non-toxic dose of 1 mg per kg body weight (bdw). Single-cell RT-PCR revealed that the majority of midbrain DA neurons expressed PTPRZ. No histological alterations were observed in the mesolimbic or nigrostriatal dopaminergic pathways in Ptprz-KO brains; however, a significant decrease was noted in brain DA turnover, suggesting functional alterations. In vivo microdialysis experiments revealed that METH-evoked DA release in the nucleus accumbens was significantly lower in Ptprz-KO mice than in wild-type mice. Consistent with this result, Ptprz-KO mice showed significantly fewer cell surface DAT as well as weaker DA uptake activity in striatal synaptosomes prepared 1 hr after the administration of METH than wild-type mice, while no significant differences were observed in the two groups treated with saline. These results indicate that the high response phenotype of Ptprz-KO mice to novelty may not be simply attributed to hyper-dopaminergic activity, and that deficits in PTPRZ reduce the effects of METH by reducing DAT activity.

AB - Methamphetamine (METH), a commonly abused drug, elevates extracellular dopamine (DA) levels by inducing DA efflux through the DA transporter (DAT). Emerging evidence in rodent models suggests that locomotor responses to a novel inescapable open field may predict behavioral responses to abused drugs; METH produces more potent stimulant effects in high responders to novelty than in low responders. We herein found that mice deficient in protein tyrosine phosphatase receptor type Z (Ptprz-KO) exhibited an enhanced behavioral response to novelty; however, METH-induced hyperlocomotion was significantly lower in Ptprz-KO than in wild-type mice when METH was administered at a non-toxic dose of 1 mg per kg body weight (bdw). Single-cell RT-PCR revealed that the majority of midbrain DA neurons expressed PTPRZ. No histological alterations were observed in the mesolimbic or nigrostriatal dopaminergic pathways in Ptprz-KO brains; however, a significant decrease was noted in brain DA turnover, suggesting functional alterations. In vivo microdialysis experiments revealed that METH-evoked DA release in the nucleus accumbens was significantly lower in Ptprz-KO mice than in wild-type mice. Consistent with this result, Ptprz-KO mice showed significantly fewer cell surface DAT as well as weaker DA uptake activity in striatal synaptosomes prepared 1 hr after the administration of METH than wild-type mice, while no significant differences were observed in the two groups treated with saline. These results indicate that the high response phenotype of Ptprz-KO mice to novelty may not be simply attributed to hyper-dopaminergic activity, and that deficits in PTPRZ reduce the effects of METH by reducing DAT activity.

UR - http://www.scopus.com/inward/record.url?scp=85070894919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070894919&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0221205

DO - 10.1371/journal.pone.0221205

M3 - Article

C2 - 31430310

AN - SCOPUS:85070894919

VL - 14

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e0221205

ER -