Microglial and astrocytic reactions prior to onset of thalamic cell death after traumatic lesion of the rat sensorimotor cortex

Dirk M. Hermann, Günter Mies, Ryuji Hata, Konstantin Alexander Hossmann

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The temporospatial relationship between microglial and astrocytic reactions and delayed thalamic cell death was examined 1-7 days following a traumatic cold lesion of the rat sensorimotor cortex using immunocytochemistry in combination with terminal deoxynucleotidyltransferase-mediated biotinylated dUTP nick end labeling (TUNEL) of nuclear DNA fragmentation. No or only occasional TUNEL-positive cells were found in the thalamic relay nuclei up to 3 days after trauma. After 7 days, on the other hand, a considerable number of TUNEL positive cells were seen in the ventrobasal, the ventrolateral and posterior thalamic nuclei. Already 3 days after trauma, i.e., before cell injury was detectable, many protoplasmic astrocytes, which were reactive for glial fibrillary acidic protein, and ramified microglia, which were positive for complement receptor type 3b (CR3b) but negative for major histocompatibility complex (MHC) class II antigen, were noticed in the thalamus. The number of labeled astro- and microglia further increased after 7 days, when DNA fragmentation became evident. At this time, the morphology of microglia shifted towards bushy and rod-like cells, and microglia became also reactive for MHC class II antigen. Clusters of CR3b- and MHC class II-positive microglia were found in the ventrobasal thalamus. The present findings demonstrate that trauma-induced microglial and astrocytic reactions appear in the thalamus prior the onset of cell damage.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalActa Neuropathologica
Volume99
Issue number2
DOIs
Publication statusPublished - 01-01-2000

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Microglia
Cell Death
In Situ Nick-End Labeling
Major Histocompatibility Complex
Thalamus
Complement 3b Receptors
Histocompatibility Antigens Class II
Wounds and Injuries
DNA Fragmentation
Astrocytes
Posterior Thalamic Nuclei
Ventral Thalamic Nuclei
Thalamic Nuclei
DNA Nucleotidylexotransferase
Glial Fibrillary Acidic Protein
Immunohistochemistry
Sensorimotor Cortex

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Hermann, Dirk M. ; Mies, Günter ; Hata, Ryuji ; Hossmann, Konstantin Alexander. / Microglial and astrocytic reactions prior to onset of thalamic cell death after traumatic lesion of the rat sensorimotor cortex. In: Acta Neuropathologica. 2000 ; Vol. 99, No. 2. pp. 147-153.
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Microglial and astrocytic reactions prior to onset of thalamic cell death after traumatic lesion of the rat sensorimotor cortex. / Hermann, Dirk M.; Mies, Günter; Hata, Ryuji; Hossmann, Konstantin Alexander.

In: Acta Neuropathologica, Vol. 99, No. 2, 01.01.2000, p. 147-153.

Research output: Contribution to journalArticle

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