MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing

Yuka Torii; Jun ichi Kawada; Kazuhiro Horiba; Toshihiko Okumura; Takako Suzuki; Yoshinori Ito

doi:10.1007/s13365-022-01058-3

MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing

Yuka Torii, Jun ichi Kawada, Kazuhiro Horiba, Toshihiko Okumura, Takako Suzuki, Yoshinori Ito

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Primary human herpesvirus 6 (HHV-6) infection is sometimes accompanied by acute encephalopathy with reduced subcortical diffusion (AED) in immunocompetent children. We investigated exosomal microRNA (miRNA) expression profiles in cerebrospinal fluid (CSF) and sera of patients with HHV-6-associated AED (n = 5) and febrile seizure (FS) (n = 5) using high-throughput sequencing. A total of 176 and 663 miRNAs were identified in CSF and serum exosomes, respectively. Comparative analysis determined that some miRNAs (miR-381-3p, miR-155) were exclusively expressed in the CSF exosomes of AED but not of FS patients, suggesting their potential application as novel diagnostic biomarkers for AED.

Original language	English
Pages (from-to)	151-157
Number of pages	7
Journal	Journal of neurovirology
Volume	28
Issue number	1
DOIs	https://doi.org/10.1007/s13365-022-01058-3
Publication status	Published - 02-2022
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neurology
Clinical Neurology
Cellular and Molecular Neuroscience
Virology

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10.1007/s13365-022-01058-3

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title = "MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing",

abstract = "Primary human herpesvirus 6 (HHV-6) infection is sometimes accompanied by acute encephalopathy with reduced subcortical diffusion (AED) in immunocompetent children. We investigated exosomal microRNA (miRNA) expression profiles in cerebrospinal fluid (CSF) and sera of patients with HHV-6-associated AED (n = 5) and febrile seizure (FS) (n = 5) using high-throughput sequencing. A total of 176 and 663 miRNAs were identified in CSF and serum exosomes, respectively. Comparative analysis determined that some miRNAs (miR-381-3p, miR-155) were exclusively expressed in the CSF exosomes of AED but not of FS patients, suggesting their potential application as novel diagnostic biomarkers for AED.",

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T1 - MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing

AU - Torii, Yuka

AU - Kawada, Jun ichi

AU - Horiba, Kazuhiro

AU - Okumura, Toshihiko

AU - Suzuki, Takako

AU - Ito, Yoshinori

PY - 2022/2

Y1 - 2022/2

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AB - Primary human herpesvirus 6 (HHV-6) infection is sometimes accompanied by acute encephalopathy with reduced subcortical diffusion (AED) in immunocompetent children. We investigated exosomal microRNA (miRNA) expression profiles in cerebrospinal fluid (CSF) and sera of patients with HHV-6-associated AED (n = 5) and febrile seizure (FS) (n = 5) using high-throughput sequencing. A total of 176 and 663 miRNAs were identified in CSF and serum exosomes, respectively. Comparative analysis determined that some miRNAs (miR-381-3p, miR-155) were exclusively expressed in the CSF exosomes of AED but not of FS patients, suggesting their potential application as novel diagnostic biomarkers for AED.

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MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing

Abstract

All Science Journal Classification (ASJC) codes

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