Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-Immunohistochemical investigation of dendritic damage

K. Kitagawa, M. Matsumoto, M. Niinobe, K. Mikoshiba, Ryuji Hata, H. Ueda, N. Handa, R. Fukunaga, Y. Isaka, K. Kimura, T. Kamada

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

We investigated the neuronal distribution of microtubule-associated protein 2 in gerbil brain and monitored the progression of ischemic damage immunohistochemically by using this protein as a dendritic marker. The reaction for microtubule-associated protein 2 in normal gerbil brain clearly visualized neuronal soma and dendrites but other structures such as axonal bundles, glia and endothelial cells exhibited little immunoreactivity. In a reproducible gerbil model of unilateral cerebral ischemia, we could detect the ischemic lesions as early as 3 min after right common carotid occlusion at the subiculum-CA1 region of the ipsilateral hippocampus as faint loss of the reaction in the dendrites. After ischemia for 30 min. the ischemic lesions were clearly detected as loss of the reaction in the nerve cell bodies, dendrites and the neuropil in the hippocampus, cerebral cortex, thalamus and the caudoptamen. Although the mechanism for prompt disappearance of the immunohistochemical reaction for microtubule-associated protein 2 is not clear, the present investigation suggests that dendrites in the vulnerable regions may be quite susceptible to ischemic stress and that the immunohistochemical procedure for microtubule-associated protein 2 may be very useful for demonstration of dendritic damage in various pathophysiological states of the central nervous system.

Original languageEnglish
Pages (from-to)401-411
Number of pages11
JournalNeuroscience
Volume31
Issue number2
DOIs
Publication statusPublished - 01-01-1989

Fingerprint

Microtubule-Associated Proteins
Dendrites
Gerbillinae
Hippocampus
Neuropil
Brain
Carisoprodol
Brain Ischemia
Thalamus
Neuroglia
Cerebral Cortex
Ischemia
Central Nervous System
Endothelial Cells
Neurons
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Kitagawa, K. ; Matsumoto, M. ; Niinobe, M. ; Mikoshiba, K. ; Hata, Ryuji ; Ueda, H. ; Handa, N. ; Fukunaga, R. ; Isaka, Y. ; Kimura, K. ; Kamada, T. / Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-Immunohistochemical investigation of dendritic damage. In: Neuroscience. 1989 ; Vol. 31, No. 2. pp. 401-411.
@article{d9bb01101e15469c97beb6f2e42bc661,
title = "Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-Immunohistochemical investigation of dendritic damage",
abstract = "We investigated the neuronal distribution of microtubule-associated protein 2 in gerbil brain and monitored the progression of ischemic damage immunohistochemically by using this protein as a dendritic marker. The reaction for microtubule-associated protein 2 in normal gerbil brain clearly visualized neuronal soma and dendrites but other structures such as axonal bundles, glia and endothelial cells exhibited little immunoreactivity. In a reproducible gerbil model of unilateral cerebral ischemia, we could detect the ischemic lesions as early as 3 min after right common carotid occlusion at the subiculum-CA1 region of the ipsilateral hippocampus as faint loss of the reaction in the dendrites. After ischemia for 30 min. the ischemic lesions were clearly detected as loss of the reaction in the nerve cell bodies, dendrites and the neuropil in the hippocampus, cerebral cortex, thalamus and the caudoptamen. Although the mechanism for prompt disappearance of the immunohistochemical reaction for microtubule-associated protein 2 is not clear, the present investigation suggests that dendrites in the vulnerable regions may be quite susceptible to ischemic stress and that the immunohistochemical procedure for microtubule-associated protein 2 may be very useful for demonstration of dendritic damage in various pathophysiological states of the central nervous system.",
author = "K. Kitagawa and M. Matsumoto and M. Niinobe and K. Mikoshiba and Ryuji Hata and H. Ueda and N. Handa and R. Fukunaga and Y. Isaka and K. Kimura and T. Kamada",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0306-4522(89)90383-7",
language = "English",
volume = "31",
pages = "401--411",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "2",

}

Kitagawa, K, Matsumoto, M, Niinobe, M, Mikoshiba, K, Hata, R, Ueda, H, Handa, N, Fukunaga, R, Isaka, Y, Kimura, K & Kamada, T 1989, 'Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-Immunohistochemical investigation of dendritic damage', Neuroscience, vol. 31, no. 2, pp. 401-411. https://doi.org/10.1016/0306-4522(89)90383-7

Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-Immunohistochemical investigation of dendritic damage. / Kitagawa, K.; Matsumoto, M.; Niinobe, M.; Mikoshiba, K.; Hata, Ryuji; Ueda, H.; Handa, N.; Fukunaga, R.; Isaka, Y.; Kimura, K.; Kamada, T.

In: Neuroscience, Vol. 31, No. 2, 01.01.1989, p. 401-411.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microtubule-associated protein 2 as a sensitive marker for cerebral ischemic damage-Immunohistochemical investigation of dendritic damage

AU - Kitagawa, K.

AU - Matsumoto, M.

AU - Niinobe, M.

AU - Mikoshiba, K.

AU - Hata, Ryuji

AU - Ueda, H.

AU - Handa, N.

AU - Fukunaga, R.

AU - Isaka, Y.

AU - Kimura, K.

AU - Kamada, T.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - We investigated the neuronal distribution of microtubule-associated protein 2 in gerbil brain and monitored the progression of ischemic damage immunohistochemically by using this protein as a dendritic marker. The reaction for microtubule-associated protein 2 in normal gerbil brain clearly visualized neuronal soma and dendrites but other structures such as axonal bundles, glia and endothelial cells exhibited little immunoreactivity. In a reproducible gerbil model of unilateral cerebral ischemia, we could detect the ischemic lesions as early as 3 min after right common carotid occlusion at the subiculum-CA1 region of the ipsilateral hippocampus as faint loss of the reaction in the dendrites. After ischemia for 30 min. the ischemic lesions were clearly detected as loss of the reaction in the nerve cell bodies, dendrites and the neuropil in the hippocampus, cerebral cortex, thalamus and the caudoptamen. Although the mechanism for prompt disappearance of the immunohistochemical reaction for microtubule-associated protein 2 is not clear, the present investigation suggests that dendrites in the vulnerable regions may be quite susceptible to ischemic stress and that the immunohistochemical procedure for microtubule-associated protein 2 may be very useful for demonstration of dendritic damage in various pathophysiological states of the central nervous system.

AB - We investigated the neuronal distribution of microtubule-associated protein 2 in gerbil brain and monitored the progression of ischemic damage immunohistochemically by using this protein as a dendritic marker. The reaction for microtubule-associated protein 2 in normal gerbil brain clearly visualized neuronal soma and dendrites but other structures such as axonal bundles, glia and endothelial cells exhibited little immunoreactivity. In a reproducible gerbil model of unilateral cerebral ischemia, we could detect the ischemic lesions as early as 3 min after right common carotid occlusion at the subiculum-CA1 region of the ipsilateral hippocampus as faint loss of the reaction in the dendrites. After ischemia for 30 min. the ischemic lesions were clearly detected as loss of the reaction in the nerve cell bodies, dendrites and the neuropil in the hippocampus, cerebral cortex, thalamus and the caudoptamen. Although the mechanism for prompt disappearance of the immunohistochemical reaction for microtubule-associated protein 2 is not clear, the present investigation suggests that dendrites in the vulnerable regions may be quite susceptible to ischemic stress and that the immunohistochemical procedure for microtubule-associated protein 2 may be very useful for demonstration of dendritic damage in various pathophysiological states of the central nervous system.

UR - http://www.scopus.com/inward/record.url?scp=0024420534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024420534&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(89)90383-7

DO - 10.1016/0306-4522(89)90383-7

M3 - Article

C2 - 2797444

AN - SCOPUS:0024420534

VL - 31

SP - 401

EP - 411

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 2

ER -