Midkine is involved in neutrophil infiltration into the tubulointerstitium in ischemic renal injury

W. Sato, K. Kadomatsu, Yukio Yuzawa, H. Muramatsu, N. Hotta, S. Matsuo, T. Muramatsu

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Midkine (MK) is a multifunctional heparin-binding protein and promotes migration of neutrophils, macrophages, and neurons. In the normal mouse kidney, MK is expressed in the proximal tubules. After renal ischemic reperfusion injury, its expression in proximal tubules was increased. Immediate increase of MK expression was found when renal proximal tubular epithelial cells in culture were exposed to 5 mM H2O2. Histologically defined tubulointerstitial damage was less severe in MK-deficient (Mdk-/-) than in wild-type (Mdk+/+) mice at 2 and 7 days after ischemic reperfusion injury. Within 2 days after ischemic injury, inflammatory leukocytes, of which neutrophils were the major population, were recruited to the tubulointerstitium. The numbers of infiltrating neutrophils and also macrophages were lower in Mdk-/- than in Mdk+/+ mice. Induction of macrophage inflammatory protein-2 and macrophage chemotactic protein-1, chemokines for neutrophils and macrophages, respectively, were also suppressed in Mdk-/- mice. Furthermore, renal tubular epithelial cells in culture expressed macrophage inflammatory protein-2 in response to exogenous MK administration. These results suggested that MK enhances migration of inflammatory cells upon ischemic injury of the kidney directly and also through induction of chemokines, and contributes to the augmentation of ischemic tissue damage.

Original languageEnglish
Pages (from-to)3463-3469
Number of pages7
JournalJournal of Immunology
Volume167
Issue number6
DOIs
Publication statusPublished - 15-09-2001
Externally publishedYes

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Neutrophil Infiltration
Kidney
Wounds and Injuries
Neutrophils
Macrophages
Chemokine CXCL2
Reperfusion Injury
Chemokines
Cell Culture Techniques
Epithelial Cells
Cell Movement
midkine
Leukocytes
Neurons
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Sato, W. ; Kadomatsu, K. ; Yuzawa, Yukio ; Muramatsu, H. ; Hotta, N. ; Matsuo, S. ; Muramatsu, T. / Midkine is involved in neutrophil infiltration into the tubulointerstitium in ischemic renal injury. In: Journal of Immunology. 2001 ; Vol. 167, No. 6. pp. 3463-3469.
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Sato, W, Kadomatsu, K, Yuzawa, Y, Muramatsu, H, Hotta, N, Matsuo, S & Muramatsu, T 2001, 'Midkine is involved in neutrophil infiltration into the tubulointerstitium in ischemic renal injury', Journal of Immunology, vol. 167, no. 6, pp. 3463-3469. https://doi.org/10.4049/jimmunol.167.6.3463

Midkine is involved in neutrophil infiltration into the tubulointerstitium in ischemic renal injury. / Sato, W.; Kadomatsu, K.; Yuzawa, Yukio; Muramatsu, H.; Hotta, N.; Matsuo, S.; Muramatsu, T.

In: Journal of Immunology, Vol. 167, No. 6, 15.09.2001, p. 3463-3469.

Research output: Contribution to journalArticle

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T1 - Midkine is involved in neutrophil infiltration into the tubulointerstitium in ischemic renal injury

AU - Sato, W.

AU - Kadomatsu, K.

AU - Yuzawa, Yukio

AU - Muramatsu, H.

AU - Hotta, N.

AU - Matsuo, S.

AU - Muramatsu, T.

PY - 2001/9/15

Y1 - 2001/9/15

N2 - Midkine (MK) is a multifunctional heparin-binding protein and promotes migration of neutrophils, macrophages, and neurons. In the normal mouse kidney, MK is expressed in the proximal tubules. After renal ischemic reperfusion injury, its expression in proximal tubules was increased. Immediate increase of MK expression was found when renal proximal tubular epithelial cells in culture were exposed to 5 mM H2O2. Histologically defined tubulointerstitial damage was less severe in MK-deficient (Mdk-/-) than in wild-type (Mdk+/+) mice at 2 and 7 days after ischemic reperfusion injury. Within 2 days after ischemic injury, inflammatory leukocytes, of which neutrophils were the major population, were recruited to the tubulointerstitium. The numbers of infiltrating neutrophils and also macrophages were lower in Mdk-/- than in Mdk+/+ mice. Induction of macrophage inflammatory protein-2 and macrophage chemotactic protein-1, chemokines for neutrophils and macrophages, respectively, were also suppressed in Mdk-/- mice. Furthermore, renal tubular epithelial cells in culture expressed macrophage inflammatory protein-2 in response to exogenous MK administration. These results suggested that MK enhances migration of inflammatory cells upon ischemic injury of the kidney directly and also through induction of chemokines, and contributes to the augmentation of ischemic tissue damage.

AB - Midkine (MK) is a multifunctional heparin-binding protein and promotes migration of neutrophils, macrophages, and neurons. In the normal mouse kidney, MK is expressed in the proximal tubules. After renal ischemic reperfusion injury, its expression in proximal tubules was increased. Immediate increase of MK expression was found when renal proximal tubular epithelial cells in culture were exposed to 5 mM H2O2. Histologically defined tubulointerstitial damage was less severe in MK-deficient (Mdk-/-) than in wild-type (Mdk+/+) mice at 2 and 7 days after ischemic reperfusion injury. Within 2 days after ischemic injury, inflammatory leukocytes, of which neutrophils were the major population, were recruited to the tubulointerstitium. The numbers of infiltrating neutrophils and also macrophages were lower in Mdk-/- than in Mdk+/+ mice. Induction of macrophage inflammatory protein-2 and macrophage chemotactic protein-1, chemokines for neutrophils and macrophages, respectively, were also suppressed in Mdk-/- mice. Furthermore, renal tubular epithelial cells in culture expressed macrophage inflammatory protein-2 in response to exogenous MK administration. These results suggested that MK enhances migration of inflammatory cells upon ischemic injury of the kidney directly and also through induction of chemokines, and contributes to the augmentation of ischemic tissue damage.

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