Mitogen-activated protein kinase phosphatase 1 negatively regulates MAPK signaling in mouse hypothalamus

Koichi Adachi, Motomitsu Goto, Takeshi Onoue, Taku Tsunekawa, Miyuki Shibata, Shigeru Hagimoto, Yoshihiro Ito, Ryoichi Banno, Hidetaka Suga, Yoshihisa Sugimura, Yutaka Oiso, Hiroshi Arima

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7 Citations (Scopus)

Abstract

Mitogen-activated protein kinase phosphatase 1 (MKP-1) is shown to negatively regulate MAPK signaling in various peripheral tissues as well as the central nervous system such as cortex, striatum and hippocampus. In this study, we examined whether MKP-1 regulates MAPK signaling in the mouse hypothalamus. Intraperitoneal injection of TNFα significantly increased MKP-1 mRNA expression in paraventricular and arcuate nuclei in the hypothalamus. TNFα treatment induced increases in MKP-1 expression at both mRNA and protein levels, accompanied by the inactivation of MAPK signaling in mouse hypothalamic explants. Inhibition of MKP-1 by its inhibitor or siRNA increased MAPK activity in the explants. Our data indicate that MKP-1 negatively regulates MAPK signaling in the mouse hypothalamus.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalNeuroscience Letters
Volume569
DOIs
Publication statusPublished - 21-05-2014

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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    Adachi, K., Goto, M., Onoue, T., Tsunekawa, T., Shibata, M., Hagimoto, S., Ito, Y., Banno, R., Suga, H., Sugimura, Y., Oiso, Y., & Arima, H. (2014). Mitogen-activated protein kinase phosphatase 1 negatively regulates MAPK signaling in mouse hypothalamus. Neuroscience Letters, 569, 49-54. https://doi.org/10.1016/j.neulet.2014.03.032