TY - JOUR
T1 - Mitogen-activated protein kinase phosphatase 1 negatively regulates MAPK signaling in mouse hypothalamus
AU - Adachi, Koichi
AU - Goto, Motomitsu
AU - Onoue, Takeshi
AU - Tsunekawa, Taku
AU - Shibata, Miyuki
AU - Hagimoto, Shigeru
AU - Ito, Yoshihiro
AU - Banno, Ryoichi
AU - Suga, Hidetaka
AU - Sugimura, Yoshihisa
AU - Oiso, Yutaka
AU - Arima, Hiroshi
PY - 2014/5/21
Y1 - 2014/5/21
N2 - Mitogen-activated protein kinase phosphatase 1 (MKP-1) is shown to negatively regulate MAPK signaling in various peripheral tissues as well as the central nervous system such as cortex, striatum and hippocampus. In this study, we examined whether MKP-1 regulates MAPK signaling in the mouse hypothalamus. Intraperitoneal injection of TNFα significantly increased MKP-1 mRNA expression in paraventricular and arcuate nuclei in the hypothalamus. TNFα treatment induced increases in MKP-1 expression at both mRNA and protein levels, accompanied by the inactivation of MAPK signaling in mouse hypothalamic explants. Inhibition of MKP-1 by its inhibitor or siRNA increased MAPK activity in the explants. Our data indicate that MKP-1 negatively regulates MAPK signaling in the mouse hypothalamus.
AB - Mitogen-activated protein kinase phosphatase 1 (MKP-1) is shown to negatively regulate MAPK signaling in various peripheral tissues as well as the central nervous system such as cortex, striatum and hippocampus. In this study, we examined whether MKP-1 regulates MAPK signaling in the mouse hypothalamus. Intraperitoneal injection of TNFα significantly increased MKP-1 mRNA expression in paraventricular and arcuate nuclei in the hypothalamus. TNFα treatment induced increases in MKP-1 expression at both mRNA and protein levels, accompanied by the inactivation of MAPK signaling in mouse hypothalamic explants. Inhibition of MKP-1 by its inhibitor or siRNA increased MAPK activity in the explants. Our data indicate that MKP-1 negatively regulates MAPK signaling in the mouse hypothalamus.
UR - https://www.scopus.com/pages/publications/84898771510
UR - https://www.scopus.com/pages/publications/84898771510#tab=citedBy
U2 - 10.1016/j.neulet.2014.03.032
DO - 10.1016/j.neulet.2014.03.032
M3 - Article
C2 - 24686178
AN - SCOPUS:84898771510
SN - 0304-3940
VL - 569
SP - 49
EP - 54
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -