Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors

Kouichi Ohta; Kunio Shimazu; Satoru Komatsumoto; Nobuo Araki; Mamoru Shibata; Yasuo Fukuuchi

doi:10.1097/00001756-199403000-00007

Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors

Kouichi Ohta, Kunio Shimazu, Satoru Komatsumoto, Nobuo Araki, Mamoru Shibata, Yasuo Fukuuchi

Research output: Contribution to journal › Article › peer-review

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Abstract

The effects of N^G-substituted L-arginine (ARG) analogues on striatal ARG and citrulline (CIT) levels were investigated using in vivo microdialysis technique. A microdialysis probe was implanted into the striatum of anaesthetized Sprague-Dawley rats. Direct intrastriatal perfusion with 1 mM N^G-nitro-L-arginine methyl ester (n = 8) increased striatal ARG release and decreased CIT release, suggesting suppressed NO synthase activity in the tissue. On the other hand, 1 mM N^G-monomethyl-L-argi-nine (L-NMMA) (n = 6) evoked a persistent increase in both ARG and CIT. Considering that 4-320 μM L-ARG (n = 8) failed to increase CIT formation, CIT seems to be synthesized in the striatal tissue from L-NMMA by the enzyme that has been demonstrated in the kidney and aortic endothelium (N^G, N^G-dimethylarginine dimethyl-aminohydrolase).

Original language	English
Pages (from-to)	766-768
Number of pages	3
Journal	Neuroreport
Volume	5
Issue number	7
DOIs	https://doi.org/10.1097/00001756-199403000-00007
Publication status	Published - 03-1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

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title = "Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors",

abstract = "The effects of NG-substituted L-arginine (ARG) analogues on striatal ARG and citrulline (CIT) levels were investigated using in vivo microdialysis technique. A microdialysis probe was implanted into the striatum of anaesthetized Sprague-Dawley rats. Direct intrastriatal perfusion with 1 mM NG-nitro-L-arginine methyl ester (n = 8) increased striatal ARG release and decreased CIT release, suggesting suppressed NO synthase activity in the tissue. On the other hand, 1 mM NG-monomethyl-L-argi-nine (L-NMMA) (n = 6) evoked a persistent increase in both ARG and CIT. Considering that 4-320 μM L-ARG (n = 8) failed to increase CIT formation, CIT seems to be synthesized in the striatal tissue from L-NMMA by the enzyme that has been demonstrated in the kidney and aortic endothelium (NG, NG-dimethylarginine dimethyl-aminohydrolase).",

author = "Kouichi Ohta and Kunio Shimazu and Satoru Komatsumoto and Nobuo Araki and Mamoru Shibata and Yasuo Fukuuchi",

year = "1994",

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T1 - Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors

AU - Ohta, Kouichi

AU - Shimazu, Kunio

AU - Komatsumoto, Satoru

AU - Araki, Nobuo

AU - Shibata, Mamoru

AU - Fukuuchi, Yasuo

PY - 1994/3

Y1 - 1994/3

N2 - The effects of NG-substituted L-arginine (ARG) analogues on striatal ARG and citrulline (CIT) levels were investigated using in vivo microdialysis technique. A microdialysis probe was implanted into the striatum of anaesthetized Sprague-Dawley rats. Direct intrastriatal perfusion with 1 mM NG-nitro-L-arginine methyl ester (n = 8) increased striatal ARG release and decreased CIT release, suggesting suppressed NO synthase activity in the tissue. On the other hand, 1 mM NG-monomethyl-L-argi-nine (L-NMMA) (n = 6) evoked a persistent increase in both ARG and CIT. Considering that 4-320 μM L-ARG (n = 8) failed to increase CIT formation, CIT seems to be synthesized in the striatal tissue from L-NMMA by the enzyme that has been demonstrated in the kidney and aortic endothelium (NG, NG-dimethylarginine dimethyl-aminohydrolase).

AB - The effects of NG-substituted L-arginine (ARG) analogues on striatal ARG and citrulline (CIT) levels were investigated using in vivo microdialysis technique. A microdialysis probe was implanted into the striatum of anaesthetized Sprague-Dawley rats. Direct intrastriatal perfusion with 1 mM NG-nitro-L-arginine methyl ester (n = 8) increased striatal ARG release and decreased CIT release, suggesting suppressed NO synthase activity in the tissue. On the other hand, 1 mM NG-monomethyl-L-argi-nine (L-NMMA) (n = 6) evoked a persistent increase in both ARG and CIT. Considering that 4-320 μM L-ARG (n = 8) failed to increase CIT formation, CIT seems to be synthesized in the striatal tissue from L-NMMA by the enzyme that has been demonstrated in the kidney and aortic endothelium (NG, NG-dimethylarginine dimethyl-aminohydrolase).

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Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors

Abstract

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