Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens

K. Taniguchi, C. Shimazaki, N. Ochiai, E. Maruya, Yoshiki Akatsuka, E. Ashihara, T. Maekawa, M. Taniwaki, H. Saji

Research output: Contribution to journalArticle

Abstract

Clinical reports have suggested the existence of immunological tolerance to noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched allogeneic stem cell transplantation (allo-SCT). We studied the T-cell reactivity using IFN-y enzyme- linked immunospot (ELISPOT) assay in three HLA fully matched allo-SCT cases and one healthy volunteer family case. In HLA fully matched allo-SCT cases, ELISPOT assay could detect the hyporespon- siveness of T cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed that the T cells from an individual responded to B cell from his mother significantly weakly than those from an unrelated HLA-haploidentical individual. These observations suggest that our IFN-y ELISPOT assay-based method may predict the presence of immunological tolerance to NIMA.

Original languageEnglish
JournalInternational Journal of Laboratory Hematology
Volume32
Issue number1 PART.1
DOIs
Publication statusPublished - 01-02-2010
Externally publishedYes

Fingerprint

Enzyme-Linked Immunospot Assay
T-cells
HLA Antigens
Assays
Stem Cell Transplantation
Stem cells
Mothers
T-Lymphocytes
Antigens
Enzymes
B-Lymphocytes
Cells
Healthy Volunteers
Tissue Donors

All Science Journal Classification (ASJC) codes

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Taniguchi, K. ; Shimazaki, C. ; Ochiai, N. ; Maruya, E. ; Akatsuka, Yoshiki ; Ashihara, E. ; Maekawa, T. ; Taniwaki, M. ; Saji, H. / Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens. In: International Journal of Laboratory Hematology. 2010 ; Vol. 32, No. 1 PART.1.
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Taniguchi, K, Shimazaki, C, Ochiai, N, Maruya, E, Akatsuka, Y, Ashihara, E, Maekawa, T, Taniwaki, M & Saji, H 2010, 'Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens', International Journal of Laboratory Hematology, vol. 32, no. 1 PART.1. https://doi.org/10.1111/j.1751-553X.2008.01121.x

Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens. / Taniguchi, K.; Shimazaki, C.; Ochiai, N.; Maruya, E.; Akatsuka, Yoshiki; Ashihara, E.; Maekawa, T.; Taniwaki, M.; Saji, H.

In: International Journal of Laboratory Hematology, Vol. 32, No. 1 PART.1, 01.02.2010.

Research output: Contribution to journalArticle

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AU - Taniguchi, K.

AU - Shimazaki, C.

AU - Ochiai, N.

AU - Maruya, E.

AU - Akatsuka, Yoshiki

AU - Ashihara, E.

AU - Maekawa, T.

AU - Taniwaki, M.

AU - Saji, H.

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