Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens

K. Taniguchi, C. Shimazaki, N. Ochiai, E. Maruya, Y. Akatsuka, E. Ashihara, T. Maekawa, M. Taniwaki, H. Saji

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Abstract

Clinical reports have suggested the existence of immunological tolerance to noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched allogeneic stem cell transplantation (allo-SCT). We studied the T-cell reactivity using IFN-y enzyme- linked immunospot (ELISPOT) assay in three HLA fully matched allo-SCT cases and one healthy volunteer family case. In HLA fully matched allo-SCT cases, ELISPOT assay could detect the hyporespon- siveness of T cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed that the T cells from an individual responded to B cell from his mother significantly weakly than those from an unrelated HLA-haploidentical individual. These observations suggest that our IFN-y ELISPOT assay-based method may predict the presence of immunological tolerance to NIMA.

Original languageEnglish
Pages (from-to)e163-e168
JournalInternational Journal of Laboratory Hematology
Volume32
Issue number1 PART.1
DOIs
Publication statusPublished - 01-02-2010

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All Science Journal Classification (ASJC) codes

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Taniguchi, K., Shimazaki, C., Ochiai, N., Maruya, E., Akatsuka, Y., Ashihara, E., Maekawa, T., Taniwaki, M., & Saji, H. (2010). Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens. International Journal of Laboratory Hematology, 32(1 PART.1), e163-e168. https://doi.org/10.1111/j.1751-553X.2008.01121.x