TY - JOUR
T1 - Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens
AU - Taniguchi, K.
AU - Shimazaki, C.
AU - Ochiai, N.
AU - Maruya, E.
AU - Akatsuka, Y.
AU - Ashihara, E.
AU - Maekawa, T.
AU - Taniwaki, M.
AU - Saji, H.
PY - 2010/2
Y1 - 2010/2
N2 - Clinical reports have suggested the existence of immunological tolerance to noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched allogeneic stem cell transplantation (allo-SCT). We studied the T-cell reactivity using IFN-y enzyme- linked immunospot (ELISPOT) assay in three HLA fully matched allo-SCT cases and one healthy volunteer family case. In HLA fully matched allo-SCT cases, ELISPOT assay could detect the hyporespon- siveness of T cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed that the T cells from an individual responded to B cell from his mother significantly weakly than those from an unrelated HLA-haploidentical individual. These observations suggest that our IFN-y ELISPOT assay-based method may predict the presence of immunological tolerance to NIMA.
AB - Clinical reports have suggested the existence of immunological tolerance to noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched allogeneic stem cell transplantation (allo-SCT). We studied the T-cell reactivity using IFN-y enzyme- linked immunospot (ELISPOT) assay in three HLA fully matched allo-SCT cases and one healthy volunteer family case. In HLA fully matched allo-SCT cases, ELISPOT assay could detect the hyporespon- siveness of T cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed that the T cells from an individual responded to B cell from his mother significantly weakly than those from an unrelated HLA-haploidentical individual. These observations suggest that our IFN-y ELISPOT assay-based method may predict the presence of immunological tolerance to NIMA.
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U2 - 10.1111/j.1751-553X.2008.01121.x
DO - 10.1111/j.1751-553X.2008.01121.x
M3 - Article
C2 - 19032374
AN - SCOPUS:77249134269
SN - 1751-5521
VL - 32
SP - e163-e168
JO - International Journal of Laboratory Hematology
JF - International Journal of Laboratory Hematology
IS - 1 PART.1
ER -