Modulation of the secondary antibody response of murine lymphocytes to sheep red blood cells in vitro by neuraminidase and exoglycosidases

Makoto Nakamura, Tomoaki Yoshida, Ken ichi Isobe, Takashi Iwamoto, S. M. Jamshedur Rahman, hua Zhang Yue hua Zhang, Tadao Hasegawa, Masatoshi Ichihara, Izumi Nakashima

Research output: Contribution to journalArticle

Abstract

The effect of digestion of lymphocytes with neuraminidase and exoglycosidases on the secondary antibody response in vitro to sheep red blood cell (SRBC) antigen was tested. Treatment of spleen cells from SRBC-primed mice with 3 μg/ml of neuraminidase slightly but significantly augmented their plaque-forming cell response to SRBC, whereas treatment with 100 μg/ml of a mixture of exoglycosidases did not. Rather unexpectedly, however, treatment of the spleen cells with the mixture of both neuraminidase and exoglycosidases greatly augmented the response. This enzyme action was substrate specific inasmuch it was ablated by addition of mucin as a neuraminidase inhibitor to the enzyme mixture. The target of the enzyme activity was not glass-adherent macrophages, but was glass-non-adherent suppressor cells in the antigen-primed cell population. Evidence was provided that the phenotype of suppressor cells whose activity was ablated by the enzyme treatment was Thy-1+. It is suggested from these results that sialilated complex type oligosaccharides on antigen-primed T cells play a critical role in their suppressor activity.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalImmunology Letters
Volume29
Issue number3
DOIs
Publication statusPublished - 08-1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Modulation of the secondary antibody response of murine lymphocytes to sheep red blood cells in vitro by neuraminidase and exoglycosidases'. Together they form a unique fingerprint.

  • Cite this

    Nakamura, M., Yoshida, T., Isobe, K. I., Iwamoto, T., Jamshedur Rahman, S. M., Yue hua Zhang, H. Z., Hasegawa, T., Ichihara, M., & Nakashima, I. (1991). Modulation of the secondary antibody response of murine lymphocytes to sheep red blood cells in vitro by neuraminidase and exoglycosidases. Immunology Letters, 29(3), 235-240. https://doi.org/10.1016/0165-2478(91)90176-B