Molecular analyses of Xenopus laevis Mesp-related genes.

Keisuke Hitachi, Akiko Kondo, Hiroki Danno, Yusuke Nishimura, Koji Okabayashi, Makoto Asashima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

During vertebrate somitogenesis, somites bud off from the anterior end of the presomitic mesoderm (PSM). Mesodermal posterior (Mesp)-related genes play essential roles in somitogenesis, particularly in the definition of the somite boundary position. Among vertebrates, two types of Mesp-related genes have been identified: Mesp1 and Mesp2 in the mouse; Meso-1 and Meso-2 in the chicken; Xl-mespa and Xl-mespb (also known as Thylacine1) in the African clawed frog (Xenopus laevis); and mesp-a and mesp-b in the zebrafish. However, the functional differences between two Mesp-related genes remain unknown. In the present study, we carried out comparative analyses of the Xl-mespa and Xl-mespb genes. The amino acid sequences of the Xl-mespa and Xl-mespb proteins showed a high level of similarity. The expression of Xl-mespa started broadly in the ventrolateral mesoderm and gradually shifted to a striped pattern of expression. In contrast, Xl-mespb showed a striped pattern of expression from the start. These expression profiles completely overlapped at the PSM during somitogenesis. To investigate the functional differences between Xl-mespa and Xl-mespb in terms of target gene regulation, we carried out a luciferase assay using the murine Lunatic fringe (L-fng) promoter. Transcription of the L-fng promoter was activated more strongly by Xl-mespb than by Xl-mespa. This same pattern was observed for the murine Mesp-related proteins. These results suggest that the functional differences between the two types of Mesp-related genes are evolutionally conserved in vertebrates.

Original languageEnglish
Pages (from-to)387-394
Number of pages8
JournalIntegrative zoology
Volume4
Issue number4
DOIs
Publication statusPublished - 01-12-2009

Fingerprint

Xenopus laevis
genes
vertebrates
mice
promoter regions
luciferase
Danio rerio
amino acid sequences
proteins
transcription (genetics)
buds
chickens
assays

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Hitachi, Keisuke ; Kondo, Akiko ; Danno, Hiroki ; Nishimura, Yusuke ; Okabayashi, Koji ; Asashima, Makoto. / Molecular analyses of Xenopus laevis Mesp-related genes. In: Integrative zoology. 2009 ; Vol. 4, No. 4. pp. 387-394.
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Molecular analyses of Xenopus laevis Mesp-related genes. / Hitachi, Keisuke; Kondo, Akiko; Danno, Hiroki; Nishimura, Yusuke; Okabayashi, Koji; Asashima, Makoto.

In: Integrative zoology, Vol. 4, No. 4, 01.12.2009, p. 387-394.

Research output: Contribution to journalArticle

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