TY - JOUR
T1 - Molecular and cytogenetic characterization of the mouse ATP-binding cassette transporter Abcg4
AU - Yoshikawa, Megumi
AU - Yabuuchi, Hikaru
AU - Kuroiwa, Asato
AU - Ikegami, Yoji
AU - Sai, Yoshimichi
AU - Tamai, Ikumi
AU - Tsuji, Akira
AU - Matsuda, Yoichi
AU - Yoshida, Hisahiro
AU - Ishikawa, Toshihisa
N1 - Funding Information:
We thank Dr Yoshihide Hayashizaki and Dr Toru Yao, (Genome Science Center, RIKEN, Yokohama, Japan) for their helpful suggestions. This study was supported by a research grant (H12-Genome-026) entitled ‘Studies on the genetic polymorphism and function of pharmacokinetics-related proteins in Japanese population’ from the Japanese Ministry of Health and Welfare as well as, in part, by research grants of a NEDO project ‘Development of the hepatocyte-chip for drug toxicity evaluation’ and a Grant-in-Aid for Creative Scientific Research (No. 13NP0401) of the Japan Society for the Promotion of Science. This study is cordially dedicated to Prof. Helmut Sies (University Dusseldorf, Germany) and Prof. Naoyuki Taniguchi (Osaka University Medical School, Japan) on the occasions of their 60th birthdays.
PY - 2002/6/26
Y1 - 2002/6/26
N2 - We have cloned a new mouse ATP-binding cassette (ABC) transporter, Abcg4, from a complementary DNA (cDNA) library of mouse brain. The cloned Abcg4 cDNA encodes a protein consisting of 646 amino acids and including one ATP-binding cassette and six transmembrane domains. The Abcg4 protein exhibits high identity (96%) with human ABCG4 in terms of the amino acid sequence. Fluorescence in situ hybridization with mouse and rat chromosomes has revealed that the Abcg4 gene is located on chromosomes 9A5.3 and 8q22 distal in mouse and rat, respectively. In these loci on mouse and rat chromosomes, conserved linkage homologies were hitherto identified with human chromosome 11q23, which involves the human ABCG4 gene. The mouse Abcg4 gene as well as the human ABCG4 gene each has a total of 14 exons to encode its respective protein. High transcript levels of mouse Abcg4 were detected in mouse brain, spleen, eye, and bone marrow. Taken together, our data on the chromosomal location, gene homology, protein structure, and phylogenetic relationships strongly support the idea that mouse Abcg4 is orthologue to the human ABCG4. By functionally analyzing the mouse Abcg4 protein, we may better understand the biological role of the human ABCG4 transporter.
AB - We have cloned a new mouse ATP-binding cassette (ABC) transporter, Abcg4, from a complementary DNA (cDNA) library of mouse brain. The cloned Abcg4 cDNA encodes a protein consisting of 646 amino acids and including one ATP-binding cassette and six transmembrane domains. The Abcg4 protein exhibits high identity (96%) with human ABCG4 in terms of the amino acid sequence. Fluorescence in situ hybridization with mouse and rat chromosomes has revealed that the Abcg4 gene is located on chromosomes 9A5.3 and 8q22 distal in mouse and rat, respectively. In these loci on mouse and rat chromosomes, conserved linkage homologies were hitherto identified with human chromosome 11q23, which involves the human ABCG4 gene. The mouse Abcg4 gene as well as the human ABCG4 gene each has a total of 14 exons to encode its respective protein. High transcript levels of mouse Abcg4 were detected in mouse brain, spleen, eye, and bone marrow. Taken together, our data on the chromosomal location, gene homology, protein structure, and phylogenetic relationships strongly support the idea that mouse Abcg4 is orthologue to the human ABCG4. By functionally analyzing the mouse Abcg4 protein, we may better understand the biological role of the human ABCG4 transporter.
KW - ABCG2 (BCRP/MXR/ABCP)
KW - ABCG4
KW - ATP-binding cassette (ABC) transporter
KW - Fluorescence in situ hybridization
KW - Mouse chromosome
UR - https://www.scopus.com/pages/publications/10744231444
UR - https://www.scopus.com/inward/citedby.url?scp=10744231444&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(02)00719-9
DO - 10.1016/S0378-1119(02)00719-9
M3 - Article
C2 - 12137944
AN - SCOPUS:10744231444
SN - 0378-1119
VL - 293
SP - 67
EP - 75
JO - Gene
JF - Gene
IS - 1-2
ER -