Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis

T. Yokoyama, N. Sugimoto, K. Taniguchi, S. Komoto, T. Yuno, K. Ohta, H. Hashimoto, A. Seno, A. Ashida, M. Fujieda, S. Nishio, K. Ueno, M. Shimizu, A. Yachie

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

This is the first report showing that rotavirus infects the urinary sediment cells in immunocompetent children with rotavirus gastroenteritis. We found that inclusion-bearing cells were frequently detected in the urine samples of patients with rotavirus gastroenteritis. These cells were positive for cytokeratin, which was sometimes coexpressed with rotavirus antigen, in our immunohistochemical analysis. Moreover, in nested RT-PCR experiments, we detected rotavirus double-stranded RNA in some urine samples of patients with rotavirus gastroenteritis. We concluded that rotavirus could lead to infection of the urinary sediment cells concomitantly with rotavirus gastroenteritis.

Original languageEnglish
Pages (from-to)1190-1193
Number of pages4
JournalClinical Microbiology and Infection
Volume17
Issue number8
DOIs
Publication statusPublished - 08-2011

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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