TY - JOUR
T1 - Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis
AU - Yokoyama, T.
AU - Sugimoto, N.
AU - Taniguchi, K.
AU - Komoto, S.
AU - Yuno, T.
AU - Ohta, K.
AU - Hashimoto, H.
AU - Seno, A.
AU - Ashida, A.
AU - Fujieda, M.
AU - Nishio, S.
AU - Ueno, K.
AU - Shimizu, M.
AU - Yachie, A.
PY - 2011/8
Y1 - 2011/8
N2 - This is the first report showing that rotavirus infects the urinary sediment cells in immunocompetent children with rotavirus gastroenteritis. We found that inclusion-bearing cells were frequently detected in the urine samples of patients with rotavirus gastroenteritis. These cells were positive for cytokeratin, which was sometimes coexpressed with rotavirus antigen, in our immunohistochemical analysis. Moreover, in nested RT-PCR experiments, we detected rotavirus double-stranded RNA in some urine samples of patients with rotavirus gastroenteritis. We concluded that rotavirus could lead to infection of the urinary sediment cells concomitantly with rotavirus gastroenteritis.
AB - This is the first report showing that rotavirus infects the urinary sediment cells in immunocompetent children with rotavirus gastroenteritis. We found that inclusion-bearing cells were frequently detected in the urine samples of patients with rotavirus gastroenteritis. These cells were positive for cytokeratin, which was sometimes coexpressed with rotavirus antigen, in our immunohistochemical analysis. Moreover, in nested RT-PCR experiments, we detected rotavirus double-stranded RNA in some urine samples of patients with rotavirus gastroenteritis. We concluded that rotavirus could lead to infection of the urinary sediment cells concomitantly with rotavirus gastroenteritis.
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U2 - 10.1111/j.1469-0691.2011.03522.x
DO - 10.1111/j.1469-0691.2011.03522.x
M3 - Article
C2 - 21599796
AN - SCOPUS:79960697021
SN - 1198-743X
VL - 17
SP - 1190
EP - 1193
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 8
ER -