Molecular characterization of AID-mediated reduction of hepatitis B virus transcripts

Lusheng Que, Guangyan Liu, Kouichi Kitamura, Kousho Wakae, Yingfang Li, Hironori Nishitsuji, Saneyuki Ujino, Kunitada Shimotohno, Masamichi Muramatsu

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2 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) is the major cause of liver cirrhosis and hepatocellular carcinoma. After entering a hepatocyte, HBV forms a nuclear viral episome and produces pregenomic (pg) RNA with a stem–loop structure called an epsilon, which acts to signal encapsidation. We previously demonstrated that TGF-β upregulates activation-induced cytidine deaminase (AID) expression in hepatocytes, which in turn downregulates HBV transcripts by recruiting the RNA exosome complex. The molecular mechanism underlying AID-mediated HBV RNA reduction remains largely unclear. Here we used a pgRNA reporter system having a reporter gene within pgRNA to identify sis- and trans-acting elements in AID-mediated HBV RNA reduction. We found that the epsilon RNA and C-terminus of AID are required for AID-mediated HBV RNA reduction. Importantly, this reduction was reproduced in a hydrodynamic HBV transfection mouse model. The molecular mechanism of AID-mediated HBV RNA reduction is discussed.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalVirology
Volume510
DOIs
Publication statusPublished - 10-2017

All Science Journal Classification (ASJC) codes

  • Virology

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    Que, L., Liu, G., Kitamura, K., Wakae, K., Li, Y., Nishitsuji, H., Ujino, S., Shimotohno, K., & Muramatsu, M. (2017). Molecular characterization of AID-mediated reduction of hepatitis B virus transcripts. Virology, 510, 281-288. https://doi.org/10.1016/j.virol.2017.07.035