Molecular characterization of rat transforming growth factor-β type II receptor

Kunihiro Tsuchida; Kathy A. Lewis; Lawrence S. Mathews; Wylie W. Vale

doi:10.1006/bbrc.1993.1286

Molecular characterization of rat transforming growth factor-β type II receptor

Kunihiro Tsuchida, Kathy A. Lewis, Lawrence S. Mathews, Wylie W. Vale

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81 Citations (Scopus)

Abstract

A cDNA encoding the rat transforming growth factor-β(TGF-β) type II receptor was isolated by hybridization from a rat pituitary gland cDNA library. The rat TGF-β type II receptor comprises 567 amino acid residues with a cysteine-rich extracellular domain, a single transmembrane domain and an intracellular protein kinase domain with predicted serine/threonine specificity. The comparison of the amino acid sequences of the rat and human TGF-β type II receptors indicated that they are highly conserved particularly in the intracellular kinase domain. RNA blot hybridization and reverse-transcription polymerase chain reaction (RT-PCR) analyses showed that rat TGF-β type II receptor is widely distributed in various tissues and is expressed abundantly in the ovary and lung.

Original language	English
Pages (from-to)	790-795
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	191
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.1286
Publication status	Published - 01-05-1993
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1993.1286

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AB - A cDNA encoding the rat transforming growth factor-β(TGF-β) type II receptor was isolated by hybridization from a rat pituitary gland cDNA library. The rat TGF-β type II receptor comprises 567 amino acid residues with a cysteine-rich extracellular domain, a single transmembrane domain and an intracellular protein kinase domain with predicted serine/threonine specificity. The comparison of the amino acid sequences of the rat and human TGF-β type II receptors indicated that they are highly conserved particularly in the intracellular kinase domain. RNA blot hybridization and reverse-transcription polymerase chain reaction (RT-PCR) analyses showed that rat TGF-β type II receptor is widely distributed in various tissues and is expressed abundantly in the ovary and lung.

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Molecular characterization of rat transforming growth factor-β type II receptor

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