Molecular characterization of rat transforming growth factor-β type II receptor

Kunihiro Tsuchida, Kathy A. Lewis, Lawrence S. Mathews, Wylie W. Vale

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

A cDNA encoding the rat transforming growth factor-β(TGF-β) type II receptor was isolated by hybridization from a rat pituitary gland cDNA library. The rat TGF-β type II receptor comprises 567 amino acid residues with a cysteine-rich extracellular domain, a single transmembrane domain and an intracellular protein kinase domain with predicted serine/threonine specificity. The comparison of the amino acid sequences of the rat and human TGF-β type II receptors indicated that they are highly conserved particularly in the intracellular kinase domain. RNA blot hybridization and reverse-transcription polymerase chain reaction (RT-PCR) analyses showed that rat TGF-β type II receptor is widely distributed in various tissues and is expressed abundantly in the ovary and lung.

Original languageEnglish
Pages (from-to)790-795
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number3
DOIs
Publication statusPublished - 01-05-1993

Fingerprint

Growth Factor Receptors
Transforming Growth Factors
Rats
Amino Acids
Polymerase chain reaction
Pituitary Gland
Threonine
Transcription
Gene Library
Protein Kinases
Serine
Reverse Transcription
Cysteine
Amino Acid Sequence
Ovary
Phosphotransferases
Complementary DNA
RNA
Tissue
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tsuchida, Kunihiro ; Lewis, Kathy A. ; Mathews, Lawrence S. ; Vale, Wylie W. / Molecular characterization of rat transforming growth factor-β type II receptor. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 191, No. 3. pp. 790-795.
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Molecular characterization of rat transforming growth factor-β type II receptor. / Tsuchida, Kunihiro; Lewis, Kathy A.; Mathews, Lawrence S.; Vale, Wylie W.

In: Biochemical and Biophysical Research Communications, Vol. 191, No. 3, 01.05.1993, p. 790-795.

Research output: Contribution to journalArticle

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