Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC

Tokio Terado, Kazuhiko Okamura, Yuko Ohta, Dong Ho Shin, Sylvia L. Smith, Keiichiro Hashimoto, Tadashi Takemoto, Mayumi I. Nonaka, Hiroshi Kimura, Martin F. Flajnik, Masaru Nonaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To clarify the evolutionary origin of the linkage of the MHC class III complement genes with the MHC class I and II genes, we isolated C4 cDNA from the banded hound shark (Triakis scyllium). Upon phylogenetic tree analysis, shark C4 formed a well-supported cluster with C4 of higher vertebrates, indicating that the C3/C4 gene duplication predated the divergence of cartilaginous fish from the main line of vertebrate evolution. The deduced amino acid sequence predicted the typical C4 three-subunits chain structure, but without the histidine residue catalytic for the thioester bond, suggesting the human C4A-like specificity. The linkage analysis of the complement genes, one C4 and two factor B (Bf) genes, to the shark MHC was performed using 56 siblings from two typing panels of T. scyllium and Ginglymostoma cirratum. The C4 and one of two Bf genes showed a perfect cosegregation with the class I and II genes, whereas two recombinants were identified for the other Bf gene. These results indicate that the linkage between the complement C4 and Bf genes, as well as the linkage between these complement genes and the MHC class I and II genes were established before the emergence of cartilaginous fish >460 million years ago.

Original languageEnglish
Pages (from-to)2461-2466
Number of pages6
JournalJournal of Immunology
Volume171
Issue number5
DOIs
Publication statusPublished - 01-09-2003

Fingerprint

Sharks
Molecular Cloning
MHC Class I Genes
MHC Class II Genes
Genes
Vertebrates
Fishes
Complement C4
Gene Duplication
Complement Factor B
Histidine
Amino Acid Sequence
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Terado, Tokio ; Okamura, Kazuhiko ; Ohta, Yuko ; Shin, Dong Ho ; Smith, Sylvia L. ; Hashimoto, Keiichiro ; Takemoto, Tadashi ; Nonaka, Mayumi I. ; Kimura, Hiroshi ; Flajnik, Martin F. ; Nonaka, Masaru. / Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC. In: Journal of Immunology. 2003 ; Vol. 171, No. 5. pp. 2461-2466.
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abstract = "To clarify the evolutionary origin of the linkage of the MHC class III complement genes with the MHC class I and II genes, we isolated C4 cDNA from the banded hound shark (Triakis scyllium). Upon phylogenetic tree analysis, shark C4 formed a well-supported cluster with C4 of higher vertebrates, indicating that the C3/C4 gene duplication predated the divergence of cartilaginous fish from the main line of vertebrate evolution. The deduced amino acid sequence predicted the typical C4 three-subunits chain structure, but without the histidine residue catalytic for the thioester bond, suggesting the human C4A-like specificity. The linkage analysis of the complement genes, one C4 and two factor B (Bf) genes, to the shark MHC was performed using 56 siblings from two typing panels of T. scyllium and Ginglymostoma cirratum. The C4 and one of two Bf genes showed a perfect cosegregation with the class I and II genes, whereas two recombinants were identified for the other Bf gene. These results indicate that the linkage between the complement C4 and Bf genes, as well as the linkage between these complement genes and the MHC class I and II genes were established before the emergence of cartilaginous fish >460 million years ago.",
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Terado, T, Okamura, K, Ohta, Y, Shin, DH, Smith, SL, Hashimoto, K, Takemoto, T, Nonaka, MI, Kimura, H, Flajnik, MF & Nonaka, M 2003, 'Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC', Journal of Immunology, vol. 171, no. 5, pp. 2461-2466. https://doi.org/10.4049/jimmunol.171.5.2461

Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC. / Terado, Tokio; Okamura, Kazuhiko; Ohta, Yuko; Shin, Dong Ho; Smith, Sylvia L.; Hashimoto, Keiichiro; Takemoto, Tadashi; Nonaka, Mayumi I.; Kimura, Hiroshi; Flajnik, Martin F.; Nonaka, Masaru.

In: Journal of Immunology, Vol. 171, No. 5, 01.09.2003, p. 2461-2466.

Research output: Contribution to journalArticle

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T1 - Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC

AU - Terado, Tokio

AU - Okamura, Kazuhiko

AU - Ohta, Yuko

AU - Shin, Dong Ho

AU - Smith, Sylvia L.

AU - Hashimoto, Keiichiro

AU - Takemoto, Tadashi

AU - Nonaka, Mayumi I.

AU - Kimura, Hiroshi

AU - Flajnik, Martin F.

AU - Nonaka, Masaru

PY - 2003/9/1

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N2 - To clarify the evolutionary origin of the linkage of the MHC class III complement genes with the MHC class I and II genes, we isolated C4 cDNA from the banded hound shark (Triakis scyllium). Upon phylogenetic tree analysis, shark C4 formed a well-supported cluster with C4 of higher vertebrates, indicating that the C3/C4 gene duplication predated the divergence of cartilaginous fish from the main line of vertebrate evolution. The deduced amino acid sequence predicted the typical C4 three-subunits chain structure, but without the histidine residue catalytic for the thioester bond, suggesting the human C4A-like specificity. The linkage analysis of the complement genes, one C4 and two factor B (Bf) genes, to the shark MHC was performed using 56 siblings from two typing panels of T. scyllium and Ginglymostoma cirratum. The C4 and one of two Bf genes showed a perfect cosegregation with the class I and II genes, whereas two recombinants were identified for the other Bf gene. These results indicate that the linkage between the complement C4 and Bf genes, as well as the linkage between these complement genes and the MHC class I and II genes were established before the emergence of cartilaginous fish >460 million years ago.

AB - To clarify the evolutionary origin of the linkage of the MHC class III complement genes with the MHC class I and II genes, we isolated C4 cDNA from the banded hound shark (Triakis scyllium). Upon phylogenetic tree analysis, shark C4 formed a well-supported cluster with C4 of higher vertebrates, indicating that the C3/C4 gene duplication predated the divergence of cartilaginous fish from the main line of vertebrate evolution. The deduced amino acid sequence predicted the typical C4 three-subunits chain structure, but without the histidine residue catalytic for the thioester bond, suggesting the human C4A-like specificity. The linkage analysis of the complement genes, one C4 and two factor B (Bf) genes, to the shark MHC was performed using 56 siblings from two typing panels of T. scyllium and Ginglymostoma cirratum. The C4 and one of two Bf genes showed a perfect cosegregation with the class I and II genes, whereas two recombinants were identified for the other Bf gene. These results indicate that the linkage between the complement C4 and Bf genes, as well as the linkage between these complement genes and the MHC class I and II genes were established before the emergence of cartilaginous fish >460 million years ago.

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