TY - JOUR
T1 - Molecular cloning of precursors for TEP-1 and TEP-2
T2 - The GGNG peptide-related peptides of a prosobranch gastropod, Thais clavigera
AU - Morishita, Fumihiro
AU - Furukawa, Yasuo
AU - Kodani, Yu
AU - Minakata, Hiroyuki
AU - Horiguchi, Toshihiro
AU - Matsushima, Osamu
N1 - Funding Information:
Authors thank Professor Emeritus Hitoshi Michibata and Dr. Tatsuya Ueki at Hiroshima University for their kind support and encouragement to this work. We also thank Dr. Nobuo Yamaguchi at Mukaishima Marine Biological Laboratory, Hiroshima University for collecting animals. Liquid nitrogen used in this study was supplied by the Cryogenic and Instrumental Analysis Division of Natural Science Center for Basic Research and Development (NSCBRD). Nucleotide sequence was analyzed by Gene Science Division of NSCBRD. This work was financially supported by the grant-in-aid from the Japan Society for the Promotion of Science to F. M (No. 16570063 ) and to T. H. (No. 21248026 ) and that from the Sumitomo Foundation (No. 053480 ) to F. M.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - TEP (Thais excitatory peptide)-1 and TEP-2 are molluscan counterparts of annelidan GGNG-peptides, identified in a neogastropod, Thais clavigera (Morishita et al., 2006). We have cloned two cDNAs encoding TEP-1 and TEP-2 precursor protein, respectively, by the standard molecular cloning techniques. Predicted TEP-1 precursor protein consists of 161 amino acids, while predicted TEP-2 precursor protein has 118 amino acids. Only a single copy of TEP was found on the respective precursor. The semi-quantitative RT-PCR showed that expression of TEP-1 was high in sub-esophageal, pleural, pedal and visceral ganglia, while it was low in supra-esophageal ganglion. By contrast, expression level of TEP-2 was high in pedal and visceral ganglia. In situ hybridization visualized different subsets of TEP-1 and TEP-2 expressing neurons in Thais ganglia. For example, supra-esophageal ganglion contained many TEP-2 expressing neuron, but not TEP-1 expressing ones. These results suggest that expression of TEP-1 and TEP-2 is differently regulated in the Thais ganglia.
AB - TEP (Thais excitatory peptide)-1 and TEP-2 are molluscan counterparts of annelidan GGNG-peptides, identified in a neogastropod, Thais clavigera (Morishita et al., 2006). We have cloned two cDNAs encoding TEP-1 and TEP-2 precursor protein, respectively, by the standard molecular cloning techniques. Predicted TEP-1 precursor protein consists of 161 amino acids, while predicted TEP-2 precursor protein has 118 amino acids. Only a single copy of TEP was found on the respective precursor. The semi-quantitative RT-PCR showed that expression of TEP-1 was high in sub-esophageal, pleural, pedal and visceral ganglia, while it was low in supra-esophageal ganglion. By contrast, expression level of TEP-2 was high in pedal and visceral ganglia. In situ hybridization visualized different subsets of TEP-1 and TEP-2 expressing neurons in Thais ganglia. For example, supra-esophageal ganglion contained many TEP-2 expressing neuron, but not TEP-1 expressing ones. These results suggest that expression of TEP-1 and TEP-2 is differently regulated in the Thais ganglia.
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U2 - 10.1016/j.peptides.2014.10.009
DO - 10.1016/j.peptides.2014.10.009
M3 - Article
C2 - 25451871
AN - SCOPUS:84939935081
VL - 68
SP - 72
EP - 82
JO - Peptides
JF - Peptides
SN - 0196-9781
ER -