Molecular cloning of rabbit cytochrome b5 genes: Evidence for the occurrence of two separate genes encoding the soluble and microsomal forms

Hiromu Takematsu, Yasunori Kozutsumi, Akemi Suzuki, Toshisuke Kawasaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The rabbit genomic segments for the soluble cytochrome b5 (b5) and microsomal b5 were amplified and isolated, respectively, by means of the polymerase chain reaction using primers corresponding to various portions of the open reading frame of microsomal b5 cDNA. The DNA sequence analysis revealed that the soluble b5 gene has an extra 24 nucleotide long insert which encodes a C-terminal amino acid and a termination codon which are specific to the soluble b5. Except for the insert, the sequences of the soluble and microsomal b5 genes are identical with each other from the 5′ end to the 3′ end of the open reading frame of the microsomal b5 cDNA. Comparison of the genomic sequences with the cDNA sequences suggested that the soluble and microsomal genes are intronless within their open reading frames. These data indicate that rabbit soluble and microsomal b5 mRNAs are encoded by two highly conserved but separate genes.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume185
Issue number3
DOIs
Publication statusPublished - 30-06-1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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