TY - JOUR
T1 - Molecular cloning of rabbit cytochrome b5 genes
T2 - Evidence for the occurrence of two separate genes encoding the soluble and microsomal forms
AU - Takematsu, Hiromu
AU - Kozutsumi, Yasunori
AU - Suzuki, Akemi
AU - Kawasaki, Toshisuke
N1 - Funding Information:
Cytochrome b5 (b-j), which is an important component of electron transport systems, occurs in two forms, a microsomal form (m-form) and a soluble form (s-form). m-Form bg is found in the liver and other tissues, and is involved in the electron transport systems including the cyanide sensitive factor and many cytochrome P-450s (l-3). Contrary to the variety of biological functions displayed by m-form b5, it was thought that s-form bg only participates in the reduction of methemoglobin in erythrocytes. Recently, however, the involvement of s-form b5 in the hydroxylation of CMP-N-acetylneuraminic acid in the liver and other tissues was reported (4,5). The hydroxylation of CMP-N-acetylneuraminic acid is *This work was supported by a Grant-in-Aid for Scientiftc Research from the Ministry of Education, Science and Culture of Japan. The nucleotide sequence data mported in this paper will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Database with the following accession number D10900 for rabbit microsomal cytochrome b5 gene; D10901 for rabbit soluble cytochrome b5 gene; D10902 for rabbit soluble cytoclnome b5 cDNA. The abbreviations used are: b , cytochrome bg; PCR, polymerase chain reaction; m-form, microsomal form; s-form, solu ii le form.
PY - 1992/6/30
Y1 - 1992/6/30
N2 - The rabbit genomic segments for the soluble cytochrome b5 (b5) and microsomal b5 were amplified and isolated, respectively, by means of the polymerase chain reaction using primers corresponding to various portions of the open reading frame of microsomal b5 cDNA. The DNA sequence analysis revealed that the soluble b5 gene has an extra 24 nucleotide long insert which encodes a C-terminal amino acid and a termination codon which are specific to the soluble b5. Except for the insert, the sequences of the soluble and microsomal b5 genes are identical with each other from the 5′ end to the 3′ end of the open reading frame of the microsomal b5 cDNA. Comparison of the genomic sequences with the cDNA sequences suggested that the soluble and microsomal genes are intronless within their open reading frames. These data indicate that rabbit soluble and microsomal b5 mRNAs are encoded by two highly conserved but separate genes.
AB - The rabbit genomic segments for the soluble cytochrome b5 (b5) and microsomal b5 were amplified and isolated, respectively, by means of the polymerase chain reaction using primers corresponding to various portions of the open reading frame of microsomal b5 cDNA. The DNA sequence analysis revealed that the soluble b5 gene has an extra 24 nucleotide long insert which encodes a C-terminal amino acid and a termination codon which are specific to the soluble b5. Except for the insert, the sequences of the soluble and microsomal b5 genes are identical with each other from the 5′ end to the 3′ end of the open reading frame of the microsomal b5 cDNA. Comparison of the genomic sequences with the cDNA sequences suggested that the soluble and microsomal genes are intronless within their open reading frames. These data indicate that rabbit soluble and microsomal b5 mRNAs are encoded by two highly conserved but separate genes.
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U2 - 10.1016/0006-291X(92)91704-T
DO - 10.1016/0006-291X(92)91704-T
M3 - Article
C2 - 1627141
AN - SCOPUS:0026653072
VL - 185
SP - 845
EP - 851
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -