TY - JOUR
T1 - Molecular epidemiology of carbapenem-nonsusceptible Acinetobacter baumannii in the United States
AU - Adams-Haduch, Jennifer M.
AU - Onuoha, Ezenwa O.
AU - Bogdanovich, Tatiana
AU - Tian, Guo Bao
AU - Marschall, Jonas
AU - Urban, Carl M.
AU - Spellberg, Brad J.
AU - Rhee, Diane
AU - Halstead, Diane C.
AU - Pasculle, Anthony W.
AU - Doi, Yohei
PY - 2011/11
Y1 - 2011/11
N2 - Acinetobacter baumannii is emerging as an important nosocomial pathogen worldwide. We report molecular epidemiology of 65 carbapenem-nonsusceptible A. baumannii isolates identified from hospitals in New York, Pennsylvania, Florida, Missouri, Nevada, and California between 2008 and 2009. All isolates were subjected to pulsed-field gel electrophoresis (PFGE). Select isolates then underwent multilocus sequence typing (MLST). While the PFGE patterns tended to cluster within each hospital, sequence types (STs) belonging to the clonal complex 92 (CC92) and the pan-European clonal lineage II (EUII; worldwide clonal lineage 2) were predominant in all hospitals. Of them, ST122 and ST208 were the most common and were found in four of the six hospitals. Isolates belonging to the pan-European clonal lineages I and III were identified in one hospital each. Carbapenemase-encoding genes bla OXA-23 and/or ISAba1-bla OXA-51-like were present among the majority of isolates. These findings suggest that carbapenem-nonsusceptible A. baumannii isolates found in U.S. hospitals constitute part of the global epidemic driven by CC92, but have unique STs other than ST92, which may be spreading by means of patient transfer between health care facilities within the United States.
AB - Acinetobacter baumannii is emerging as an important nosocomial pathogen worldwide. We report molecular epidemiology of 65 carbapenem-nonsusceptible A. baumannii isolates identified from hospitals in New York, Pennsylvania, Florida, Missouri, Nevada, and California between 2008 and 2009. All isolates were subjected to pulsed-field gel electrophoresis (PFGE). Select isolates then underwent multilocus sequence typing (MLST). While the PFGE patterns tended to cluster within each hospital, sequence types (STs) belonging to the clonal complex 92 (CC92) and the pan-European clonal lineage II (EUII; worldwide clonal lineage 2) were predominant in all hospitals. Of them, ST122 and ST208 were the most common and were found in four of the six hospitals. Isolates belonging to the pan-European clonal lineages I and III were identified in one hospital each. Carbapenemase-encoding genes bla OXA-23 and/or ISAba1-bla OXA-51-like were present among the majority of isolates. These findings suggest that carbapenem-nonsusceptible A. baumannii isolates found in U.S. hospitals constitute part of the global epidemic driven by CC92, but have unique STs other than ST92, which may be spreading by means of patient transfer between health care facilities within the United States.
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U2 - 10.1128/JCM.00619-11
DO - 10.1128/JCM.00619-11
M3 - Article
C2 - 21918019
AN - SCOPUS:80355145044
SN - 0095-1137
VL - 49
SP - 3849
EP - 3854
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 11
ER -