Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in Western Pennsylvania

Hanna E. Sidjabat, David L. Paterson, Jennifer M. Adams-Haduch, Lindsay Ewan, Anthony W. Pasculle, Carlene A. Muto, Guo Bao Tian, Yohei Doi

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum β-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 and 2008. Fifty-seven isolates (81%) produced CTX-M-type ESBLs, among which CTX-M-15 was predominant (n = 46). Isolates producing CTX-M-2, -9, -14, and -65 were also identified. One CTX-M-producing isolate coproduced CMY-2 cephalosporinase. Ten isolates (14%) produced SHV-type ESBLs, either SHV-5 or SHV-7. Two isolates produced only CMY-2 or -32. Pulsed-field gel electrophoresis revealed the presence of two major clusters of CTX-M-15-producing E. coli isolates, one in phylotype B2 (n = 15) and the other in phylotype A (n = 19). Of four phylotype B2 isolates that were able to transfer the bla CTX-M-15-carrying plasmids, three showed fingerprints related (>60%) to those of plasmids from phylotype A isolates. In phylotype B2, all CTX-M-15-producing isolates, as well as three isolates producing CTX-M-14, two producing SHV-5, and one producing SHV-7, belonged to the international epidemic clone defined by serotype O25:H4 and sequence type 131. The plasmids from eight of nine CTX-M-15-producing E. coli isolates of phylotype A that were examined were highly related to each other and were also found in two isolates belonging to phylotype D, suggesting horizontal transfer of this blaCTX-M-15- carrying plasmid between phylotypes. Our findings underscore the need to further investigate the epidemiology and virulence of CTX-M-producing E. coli in the United States.

Original languageEnglish
Pages (from-to)4733-4739
Number of pages7
JournalAntimicrobial agents and chemotherapy
Volume53
Issue number11
DOIs
Publication statusPublished - 01-11-2009

Fingerprint

Molecular Epidemiology
Escherichia coli
Plasmids
Pulsed Field Gel Electrophoresis
Dermatoglyphics
Virulence
galantide
Epidemiology
Clone Cells

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Sidjabat, Hanna E. ; Paterson, David L. ; Adams-Haduch, Jennifer M. ; Ewan, Lindsay ; Pasculle, Anthony W. ; Muto, Carlene A. ; Tian, Guo Bao ; Doi, Yohei. / Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in Western Pennsylvania. In: Antimicrobial agents and chemotherapy. 2009 ; Vol. 53, No. 11. pp. 4733-4739.
@article{72ab1bc7419d4559ba9bac9237cc85f4,
title = "Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in Western Pennsylvania",
abstract = "A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum β-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 and 2008. Fifty-seven isolates (81{\%}) produced CTX-M-type ESBLs, among which CTX-M-15 was predominant (n = 46). Isolates producing CTX-M-2, -9, -14, and -65 were also identified. One CTX-M-producing isolate coproduced CMY-2 cephalosporinase. Ten isolates (14{\%}) produced SHV-type ESBLs, either SHV-5 or SHV-7. Two isolates produced only CMY-2 or -32. Pulsed-field gel electrophoresis revealed the presence of two major clusters of CTX-M-15-producing E. coli isolates, one in phylotype B2 (n = 15) and the other in phylotype A (n = 19). Of four phylotype B2 isolates that were able to transfer the bla CTX-M-15-carrying plasmids, three showed fingerprints related (>60{\%}) to those of plasmids from phylotype A isolates. In phylotype B2, all CTX-M-15-producing isolates, as well as three isolates producing CTX-M-14, two producing SHV-5, and one producing SHV-7, belonged to the international epidemic clone defined by serotype O25:H4 and sequence type 131. The plasmids from eight of nine CTX-M-15-producing E. coli isolates of phylotype A that were examined were highly related to each other and were also found in two isolates belonging to phylotype D, suggesting horizontal transfer of this blaCTX-M-15- carrying plasmid between phylotypes. Our findings underscore the need to further investigate the epidemiology and virulence of CTX-M-producing E. coli in the United States.",
author = "Sidjabat, {Hanna E.} and Paterson, {David L.} and Adams-Haduch, {Jennifer M.} and Lindsay Ewan and Pasculle, {Anthony W.} and Muto, {Carlene A.} and Tian, {Guo Bao} and Yohei Doi",
year = "2009",
month = "11",
day = "1",
doi = "10.1128/AAC.00533-09",
language = "English",
volume = "53",
pages = "4733--4739",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "11",

}

Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in Western Pennsylvania. / Sidjabat, Hanna E.; Paterson, David L.; Adams-Haduch, Jennifer M.; Ewan, Lindsay; Pasculle, Anthony W.; Muto, Carlene A.; Tian, Guo Bao; Doi, Yohei.

In: Antimicrobial agents and chemotherapy, Vol. 53, No. 11, 01.11.2009, p. 4733-4739.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in Western Pennsylvania

AU - Sidjabat, Hanna E.

AU - Paterson, David L.

AU - Adams-Haduch, Jennifer M.

AU - Ewan, Lindsay

AU - Pasculle, Anthony W.

AU - Muto, Carlene A.

AU - Tian, Guo Bao

AU - Doi, Yohei

PY - 2009/11/1

Y1 - 2009/11/1

N2 - A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum β-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 and 2008. Fifty-seven isolates (81%) produced CTX-M-type ESBLs, among which CTX-M-15 was predominant (n = 46). Isolates producing CTX-M-2, -9, -14, and -65 were also identified. One CTX-M-producing isolate coproduced CMY-2 cephalosporinase. Ten isolates (14%) produced SHV-type ESBLs, either SHV-5 or SHV-7. Two isolates produced only CMY-2 or -32. Pulsed-field gel electrophoresis revealed the presence of two major clusters of CTX-M-15-producing E. coli isolates, one in phylotype B2 (n = 15) and the other in phylotype A (n = 19). Of four phylotype B2 isolates that were able to transfer the bla CTX-M-15-carrying plasmids, three showed fingerprints related (>60%) to those of plasmids from phylotype A isolates. In phylotype B2, all CTX-M-15-producing isolates, as well as three isolates producing CTX-M-14, two producing SHV-5, and one producing SHV-7, belonged to the international epidemic clone defined by serotype O25:H4 and sequence type 131. The plasmids from eight of nine CTX-M-15-producing E. coli isolates of phylotype A that were examined were highly related to each other and were also found in two isolates belonging to phylotype D, suggesting horizontal transfer of this blaCTX-M-15- carrying plasmid between phylotypes. Our findings underscore the need to further investigate the epidemiology and virulence of CTX-M-producing E. coli in the United States.

AB - A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum β-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 and 2008. Fifty-seven isolates (81%) produced CTX-M-type ESBLs, among which CTX-M-15 was predominant (n = 46). Isolates producing CTX-M-2, -9, -14, and -65 were also identified. One CTX-M-producing isolate coproduced CMY-2 cephalosporinase. Ten isolates (14%) produced SHV-type ESBLs, either SHV-5 or SHV-7. Two isolates produced only CMY-2 or -32. Pulsed-field gel electrophoresis revealed the presence of two major clusters of CTX-M-15-producing E. coli isolates, one in phylotype B2 (n = 15) and the other in phylotype A (n = 19). Of four phylotype B2 isolates that were able to transfer the bla CTX-M-15-carrying plasmids, three showed fingerprints related (>60%) to those of plasmids from phylotype A isolates. In phylotype B2, all CTX-M-15-producing isolates, as well as three isolates producing CTX-M-14, two producing SHV-5, and one producing SHV-7, belonged to the international epidemic clone defined by serotype O25:H4 and sequence type 131. The plasmids from eight of nine CTX-M-15-producing E. coli isolates of phylotype A that were examined were highly related to each other and were also found in two isolates belonging to phylotype D, suggesting horizontal transfer of this blaCTX-M-15- carrying plasmid between phylotypes. Our findings underscore the need to further investigate the epidemiology and virulence of CTX-M-producing E. coli in the United States.

UR - http://www.scopus.com/inward/record.url?scp=70350321276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350321276&partnerID=8YFLogxK

U2 - 10.1128/AAC.00533-09

DO - 10.1128/AAC.00533-09

M3 - Article

C2 - 19687234

AN - SCOPUS:70350321276

VL - 53

SP - 4733

EP - 4739

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 11

ER -