Molecular epidemiology of rotavirus gastroenteritis in Central Kenya before vaccine introduction, 2009–2014

Ernest A. Wandera, Shah Mohammad, Satoshi Komoto, Yoshimasa Maeno, James Nyangao, Tomihiko Ide, Cyrus Kathiiko, Erick Odoyo, Takao Tsuji, Koki Taniguchi, Yoshio Ichinose

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13 Citations (Scopus)


Between July 2009 and June 2014, a total of 1,546 fecal specimens were collected from children <5 years of age with acute gastroenteritis admitted to Kiambu County Hospital, Central Kenya. The specimens were screened for group A rotavirus (RVA) using ELISA, and RVA-positive specimens were subjected to semi-nested RT-PCR to determine the G and P genotypes. RVA was detected in 429/1,546 (27.5%) fecal specimens. RVA infections occurred in all age groups <59 months, with an early peak at 6–17 months. The infections persisted year-round with distinct seasonal peaks depending on the year. G1P[8] (28%) was the most predominant genotype, followed by G9P[8] (12%), G8P[4] (7%), G1P[4] (5%), G9P[4] (4%), and G12P[6] (3%). In the yearly change of G and P genotypes, a major shift from G9P[8] to G1P[8] was found in 2012. Phylogenetic analysis of the nucleotide sequences of the VP7 and VP4 genes of seven strains with unusual G8 or P[6] showed that the VP7 nucleotide sequences of G8 were clustered in lineage 6 in which African strains are included, and that there are at least two distinct VP4 nucleotide sequences of P[6] strains. These results represent basic data on RVA strains circulating in this region before vaccine introduction. J. Med. Virol. 89:809–817, 2017.

Original languageEnglish
Pages (from-to)809-817
Number of pages9
JournalJournal of Medical Virology
Issue number5
Publication statusPublished - 01-05-2017

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology


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