Between July 2009 and June 2014, a total of 1,546 fecal specimens were collected from children <5 years of age with acute gastroenteritis admitted to Kiambu County Hospital, Central Kenya. The specimens were screened for group A rotavirus (RVA) using ELISA, and RVA-positive specimens were subjected to semi-nested RT-PCR to determine the G and P genotypes. RVA was detected in 429/1,546 (27.5%) fecal specimens. RVA infections occurred in all age groups <59 months, with an early peak at 6–17 months. The infections persisted year-round with distinct seasonal peaks depending on the year. G1P (28%) was the most predominant genotype, followed by G9P (12%), G8P (7%), G1P (5%), G9P (4%), and G12P (3%). In the yearly change of G and P genotypes, a major shift from G9P to G1P was found in 2012. Phylogenetic analysis of the nucleotide sequences of the VP7 and VP4 genes of seven strains with unusual G8 or P showed that the VP7 nucleotide sequences of G8 were clustered in lineage 6 in which African strains are included, and that there are at least two distinct VP4 nucleotide sequences of P strains. These results represent basic data on RVA strains circulating in this region before vaccine introduction. J. Med. Virol. 89:809–817, 2017.
All Science Journal Classification (ASJC) codes
- Infectious Diseases